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A method and culture medium for improve sporulation of Bacillus coagulans cell

A technology of Bacillus coagulans and a culture medium, applied in the biological field, can solve the problems of difficulty in forming spores, low spore formation rate, unfavorable industrial production, etc., and achieves the effects of easy purchase, low cost, and convenient use.

Inactive Publication Date: 2019-01-15
GUANGZHOU LYUBAIDUO BIOLOGICAL DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus coagulans is not easy to form spores in the late stage of exponential growth or in the stationary phase of Bacillus coagulans culture. Because the formation rate of spores is not high, it is not conducive to industrial production

Method used

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  • A method and culture medium for improve sporulation of Bacillus coagulans cell
  • A method and culture medium for improve sporulation of Bacillus coagulans cell
  • A method and culture medium for improve sporulation of Bacillus coagulans cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Cultivation of Bacillus coagulans with the initial 10g / L sodium lactate

[0055] 1.1 Preparation of Bacillus coagulans seed solution

[0056] Use the culture medium in Table 1, add 20g / L glucose as the sole or main carbon source, divide the prepared liquid culture medium into 500 ml Erlenmeyer flasks with 100 ml each, and seal it with an air-permeable sealing film at high temperature. (121℃) Sterilize under high pressure (0.15MPa) for 20 minutes, then sterilize by ultraviolet irradiation in a clean workbench for 20 minutes, use a pipette to suck 0.5 ml of Bacillus coagulans seed solution and directly inoculate 100 ml liquid culture in a 500 ml Erlenmeyer flask In the base, aerobic culture was carried out at a temperature of 45°C and a rotating speed of 200r / min on a shaker for 3 days and then used as Bacillus coagulans seeds.

[0057] 1.2 Preparation of 10g / L sodium lactate concentration to cultivate Bacillus coagulans

[0058] Use the culture medium in Table 1, add 10g / L sodi...

Embodiment 2

[0061] Cultivation of Bacillus coagulans with the initial 20g / L sodium lactate

[0062] 2.1 Preparation of Bacillus coagulans seed solution

[0063] Use the culture medium in Table 1, add 20g / L glucose as the sole or main carbon source, divide the prepared liquid culture medium into 500 ml Erlenmeyer flasks with 100 ml each, and seal it with an air-permeable sealing film at high temperature. (121℃) Sterilize under high pressure (0.15MPa) for 20 minutes, then sterilize by ultraviolet irradiation in a clean workbench for 20 minutes, use a pipette to suck 0.5 ml of Bacillus coagulans seed solution and directly inoculate 100 ml liquid culture in a 500 ml Erlenmeyer flask In the base, aerobic culture was carried out at a temperature of 45°C and a rotating speed of 200r / min on a shaker for 3 days and then used as Bacillus coagulans seeds.

[0064] 2.2 Preparation of 20g / L sodium lactate concentration to cultivate Bacillus coagulans

[0065] Use the culture medium in Table 1, add 20g / L sodi...

Embodiment 3

[0068] Cultivation of Bacillus coagulans with the initial 30g / L sodium lactate

[0069] 3.1 Preparation of Bacillus coagulans seed solution

[0070] Use the culture medium in Table 1, add 20g / L glucose as the sole or main carbon source, divide the prepared liquid culture medium into 500 ml Erlenmeyer flasks with 100 ml each, and seal it with an air-permeable sealing film at high temperature. (121℃) Sterilize under high pressure (0.15MPa) for 20 minutes, then sterilize by ultraviolet irradiation in a clean workbench for 20 minutes, use a pipette to suck 0.5 ml of Bacillus coagulans seed solution and directly inoculate 100 ml liquid culture in a 500 ml Erlenmeyer flask In the base, aerobic culture was carried out at a temperature of 45°C and a rotating speed of 200r / min on a shaker for 3 days and then used as Bacillus coagulans seeds.

[0071] 3.2 Preparation of 30g / L sodium lactate concentration to cultivate Bacillus coagulans

[0072] Use the culture medium in Table 1, add 30g / L sodi...

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Abstract

The invention belongs to the field of biotechnology, a method for promote sporulation of cells of Bacillus coagulans by use lactate and / or lactic acid as a sole or main carbon source for growth of that bacillus coagulans comprises culturing the bacillus coagulans in a culture medium consist of yeast extract 10.0 g / L, aerobically culturing the bacillus coagulans in a culture medium consisting of yeast extract 10.0 g / L, and culturing the bacillus coagulans in an aerobic culture medium containing yeast extract 10.0 g / L. Beef extract 10.0 g / L; Peptone 10.0 g / L; 1.5 g / L sodium bicarbonate; 1.5 g / Lpotassium dihydrogen phosphate; 1.0 g / L magnesium sulfate heptahydrate; 0.1 g / L manganese sulfate; 1.0 g / L calcium chloride; 1L of deionize water; Lactate and / or lactic acid content of 10-30g / L. Bacillus coagulans cell can be promoted to form spore in that process of culturing Bacillus coagulans, and the spore formation rate is high than 90%.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a method for using lactate and / or lactic acid as the sole or main carbon source for the growth of Bacillus coagulans to promote the formation of spores of its cells. Background technique [0002] Bacillus coagulans taxonomy belongs to the phylum Hard (or thick) wall bacteria, Bacillus genus, the cells are rod-shaped, Gram-positive, terminal spores, without flagella, can decompose sugars, homo-fermented to produce lactic acid . The optimum growth temperature of Bacillus coagulans is 45-50°C, and the optimum pH is 6.6-7.0. It can promote the balance of microbial flora in the human intestines, improve the body's immunity and disease resistance. Bacillus coagulans can not only secrete amylase and protease to promote the digestion and absorption of nutrients by humans and animals, but also produce B vitamins, amino acids and short-chain fatty acids and other life-active substances ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N3/00C12R1/07
CPCC12N1/20C12N3/00
Inventor 孙建新徐中文张金燕闫海李锴骁
Owner GUANGZHOU LYUBAIDUO BIOLOGICAL DEV CO LTD
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