28 results about "Lactate concentration" patented technology

This invention provides a method for testing the content of lactic acid and water in an aqueous lactic acid solution, comprising the following steps: preparing standard sample solutions with different lactic acid concentrations using lactic acid and water; testing the density of each standard sample solution using a densitometer to obtain the relationship between the density and the lactic acid concentration; testing the density of the aqueous lactic acid solution to be tested using the densitometer; and determining the lactic acid content and water content in the aqueous lactic acid solution to be tested based on the density of the aqueous lactic acid solution to be tested and the relationship between the density and the lactic acid concentration. This invention solves the problem of existing technologies that cannot accurately, conveniently, efficiently, and cost-effectively detect the content of lactic acid and water in an aqueous lactic acid solution simultaneously.

The invention provides application of an intracellular lactic acid level increasing agent in preparation of a drug for enhancing sensitivity of BRAF V600E mutant tumors to a BRAF inhibitor or in preparation of a drug for treating BRAF V600E mutant tumors resistant to the BRAF inhibitor. According to the invention, the intracellular lactic acid concentration is improved by using an intracellular lactic acid level improver, SUMOylation of BRAFV600E protein is promoted, and the BRAFV600E protein is locked in an open drug sensitive conformation; in-vivo and in-vitro experiments prove that the combination of the MCT4 inhibitor and the BRAF inhibitor (such as vimofenib) has a remarkable synergistic anti-tumor effect, drug resistance can be effectively relieved, and a new treatment strategy is provided for refractory colorectal cancer and the like.

The invention relates to a method for determining a blood-related, individual anaerobic threshold (IAT) of a test subject (10). For this purpose, first data (D1) is obtained comprising performance values (P) describing a performance of the test subject (10), time values (T) describing a respective associated detection time and respective associated saliva lactate concentration values (KS). The first data (D1) exceeds a maximum saliva lactate concentration value (KS). The blood-related, individual anaerobic threshold (IAT) is obtained on the basis of the first data (D1) and using a data model (M). The invention also relates to a method for monitoring a blood-related, individual anaerobic threshold (IAT) of a test subject (10). The invention further relates to a method for training a data model (M) for determining a blood-related, individual anaerobic threshold (IAT). The invention further relates to a data-processing device (22), to a computer program (32), and to a computer-readable medium (30). Furthermore, the invention relates to a system (16) for determining a blood-related, individual anaerobic threshold (IAT) and to a system for monitoring a blood-related, individual anaerobic threshold (IAT).

This invention relates to the field of multi-organ dysfunction (MOD) early warning technology, specifically to an MOD early warning method for patients with severe burns. The method includes the following steps: acquiring heart rate, mean arterial pressure, respiratory rate, blood oxygen saturation, lactate concentration, and urine output per unit time; calculating the rate of lactate change at adjacent time points to form a sequence; comparing the rate of change across time intervals to obtain the difference in change; combining heart rate and blood oxygen saturation to calculate circulatory load; and counting the number of early warning triggers that meet risk conditions. In this invention, by calculating the rate of change of lactate concentration at adjacent time points and combining it with interval direction statistics, tissue metabolic changes show trend characteristics; the rate of change of urine output combined with mean arterial pressure forms a record of renal perfusion changes, providing a continuous characterization of perfusion status; heart rate and blood oxygen saturation are used to calculate circulatory load; multiple physiological signals are linked for judgment; and recurring abnormalities are expressed through the number of triggers. This improves the stability and predictability of MOD risk identification in critically burned patients.

The invention relates to the technical field of biosensors, in particular to an implantable biosensor protected by a flexible nano-composite film and a preparation method thereof, and the biosensor comprises a current acquisition module, an impedance monitoring module, a derivative adjustment module, a trend construction module and a trend threshold classification module. According to the invention, the current is sampled in real time, the current range differences in a plurality of continuous time periods are extracted, and the dynamic identification of the derivative direction is combined, so that a fine-grained time sequence change judgment mechanism is formed, and the monitoring of the lactic acid concentration change has relatively strong dynamic response capability. A multi-modal fusion processing mode of an impedance value and a derivative is introduced, and adaptive adjustment of current derivative prediction can be realized under the electric signal difference between different bone tissue structures, so that the recognition precision of a lactic acid signal in a complex tissue environment is improved. And the derivative correction sequence is linked with direction offset analysis, so that the response sensitivity to the initial stage of trend change is enhanced.

The cell culture method comprises a step for culturing cells (8) in a culture solution (10) that satisfies at least one of the following conditions (I) and (II): Condition (I): the lactic acid concentration in the culture solution is less than 7.5 mM; condition (II): the ammonia concentration in the culture solution is less than 2.5 mM.

The invention discloses a method for solving the problem of lactic acid accumulation of senescent cells in the later culture period, which comprises the following steps of: adding nicotinamide into a culture system in the later culture period of fed-batch culture of mammalian cells to eliminate a lactic acid tail-raising state, so that lactic acid is continuously maintained in a low-level stable state; the lactic acid tail raising means that the concentration of lactic acid is increased again after cells enter a stable phase or a decline phase, and the total concentration of nicotinamide added in a culture system is 5 mM to 14 mM. According to the method, the lactic acid tail raising phenomenon in the later period of cell culture is precisely regulated and controlled, secondary accumulation of lactic acid of senescent cells is inhibited, the low lactic acid level is maintained, serious negative effects on cell growth and motility rate are avoided, the yield and quality stability of target protein are guaranteed, the process robustness is enhanced, the industrial amplification risk is reduced, and the method is suitable for industrial production. And a method library for regulating and controlling lactic acid in a protein biological medicine production process is enriched.

The invention discloses a self-flocculation fermentation lactobacillus mucus LC01 with high lactic acid yield, the preservation number of the self-flocculation fermentation lactobacillus mucus LC01 is CGMCC (China General Microbiological Culture Collection Center) No.32545, and the self-flocculation fermentation lactobacillus mucus LC01 has the capability of efficiently producing lactic acid, acetic acid and other short-chain fatty acids, has better tolerance to low pH (Potential of Hydrogen), high pressure, oxygen stress and the like, and has rapid self-flocculation capability. The invention also discloses an application of the fermented lactobacillus mucus in short-chain fatty acid production, the fermentation conditions are as follows: the temperature is 37 DEG C, the speed is 200rpm, the final lactic acid concentration is 6.88 mg / mL, and the final acetic acid concentration is 4.18 mg / mL; and rapid separation of thalli and fermentation liquor is realized by utilizing the self-flocculation capability of the strain at the fermentation end point.

The application belongs to the technical field of microbial application, and relates to a chiral L-lactic acid production engineering bacterium capable of selectively degrading racemic D-lactic acid and application of the chiral L-lactic acid production engineering bacterium. Pediococcus acidilactici The strain is classified and named as Pediococcus acidilactici LJ2071 and is preserved in the China Center for Type Culture Collection, has a preservation number of CCTCC M 20251230 and a preservation date of May 30, 2025. The strain has the ability to efficiently metabolize various types of biomass-derived monosaccharides and synthesize high-concentration and high-chirality L-lactic acid, has a certain degradation capacity for D-lactic acid contained in the fermentation raw material itself, and can use raw material containing a certain amount of racemic lactic acid to produce high-chirality L-lactic acid. When the concentration of racemic D-lactic acid in the fermentation liquor is not more than 1.5 g / L, the chirality of the L-lactic acid product is greater than 99.5%, and the L-lactic acid product can be used as a poly-lactic acid material polymerization monomer.

Certain aspects of the present disclosure relate generally to a method for identifying a risk of sepsis in a body of a patient. The method includes measuring lactate concentrations associated with the body over one or more time periods. The method further includes identifying the risk of sepsis based on the lactate concentrations.

The present disclosure provides a means for noninvasively evaluating fatigue for purposes such as designing training intensity and evaluating training effects. A method for evaluating fatigue of a subject being tested according to the present disclosure is characterized in that a measurement step for measuring a concentration of lactic acid in tear fluid collected from the subject being tested is included, and that the concentration of the lactic acid in the tear fluid is an index for evaluating fatigue of the subject being tested. The present disclosure further relates to a tear-inducing component generation kit for use in the method for evaluating fatigue of a subject being tested, the tear-inducing component generation kit comprising LFS, alliinase, and PRENCSO. The present disclosure further relates to a system for evaluating fatigue of a subject being tested in which a measured value of a concentration of lactic acid in tear fluid of the subject being tested is compared with a reference value to determine information related to a degree of fatigue of the subject being tested.

Disclosed herein is directed to a lactate oxidase variant that exhibits reduced affinity to lactate as compared to a wild-type lactate oxidase. The lactate oxidase variant has one or more amino acid substitutions occurring at positions 95, 96, and / or 175 of the wild-type amino acid sequence. Also disclosed herein is a method for detecting and quantifying lactate in various liquid samples by using the present lactate oxidase variant. The method mainly includes steps of contacting the liquid sample with said lactate oxidase variant; measuring a current generated by the reaction between the lactate oxidase variant and the lactate in the liquid sample; and determining the concentration of lactate in the liquid sample by interpolating or extrapolating the current with that of a control sample having a known concentration of lactate.

The invention relates to a preparation method of a lactic acid (lactic acid) detection kit by using a chemiluminescence immunoassay technology. As lactic acid is small in molecular weight and poor in immunogenicity, when a corresponding antibody is prepared, firstly, a proper derivative site needs to be selected, and a derived hapten is coupled with a specific macromolecular carrier to prepare a complete antigen. According to the invention, the antibody with good specificity and strong affinity is successfully prepared by designing and transforming the hapten. The invention mainly relates to design and synthesis of a lactic acid hapten, preparation of a lactic acid complete antigen and an anti-lactic acid antibody, a method for measuring the concentration of lactic acid, and composition and components of a reagent. The lactic acid derivative disclosed by the invention has a structure as shown in a formula (I) which is described in the specification.

The invention relates to the technical field of medical detection, in particular to a wireless lactic acid sensor and bone infection monitoring system based on direct electron transferase, and the system comprises an enzymatic activity dynamic control module, a fluid shear lactic acid screening module, a node lactic acid abnormity association module, a bone infection monitoring module and an infection space mapping module. According to the invention, by monitoring and analyzing the correlation between an initial current increasing signal and the local temperature change of the micro-area, the enzyme activity can be effectively controlled and optimized, the detection accuracy is increased, lactic acid molecules are screened by adopting a fluid shear force gradient field, the lactic acid monitoring efficiency and resolution are further enhanced, and by designing the local micro-fluidic area, the detection accuracy is improved. The distribution of lactic acid molecules on the surface of the sensor can be more accurately controlled and predicted, more precise biochemical reaction monitoring is realized, the change trend of lactic acid concentration is combined with a bone tissue space model, a scientific basis is provided for accurately positioning a bone infection area, and the accuracy and effectiveness of early diagnosis are remarkably improved.

The invention relates to the field of biosensing, in particular to a genetic coding fluorescent probe for detecting lactic acid change in autophagosome and application of the genetic coding fluorescent probe. The invention discloses a genetic coding fluorescent probe for detecting lactic acid change in an autophagy body, which comprises an FRET pair, a lactic acid sensing structural domain and an autophagy body targeting structural domain and is used for specifically detecting the lactic acid concentration change in the autophagy body in real time, when the lactic acid concentration in the autophagy body changes, the conformation of the probe changes, and the change of the lactic acid concentration in the autophagy body changes. As a result, the fluorescence resonance energy transfer efficiency changes, and dynamic, non-invasive and high-temporal-spatial-resolution detection of the concentration of the lactic acid in the autophagy body can be realized by monitoring the change of the FRET ratio.

The present disclosure provides a means for non-invasively evaluating fatigue for purposes such as training intensity design and training effect evaluation.SOLUTION: A method for evaluating fatigue of a test subject according to the present disclosure includes a measurement step of measuring a concentration of lactic acid in a tear collected from the test subject, in which the concentration of the lactic acid in the tear is an index for evaluating fatigue of the test subject. The present disclosure further relates to a lacrimatory component generating kit for use in a method for evaluating fatigue of a test subject, comprising LFS, alliinase, and PRENCSO. The present disclosure further relates to a system for assessing fatigue of a subject, wherein a measured value of the concentration of lactic acid in tear fluid of the subject is compared with a reference value to determine information of the degree of fatigue of the subject.SELECTED DRAWING: Figure 1

A composite material contains: bovine serum albumin modified with neutral red; single-walled carbon nanotube inclusions; and lactate oxidase enzyme inclusions. A device for quantitatively assessing lactate content in body fluids comprises a working electrode, the surface of which is coated with the composite material according to the claimed invention. The technical result is that of increasing the upper limit of the range of detectable concentrations of lactate in body fluids to 57 mM, as well as reducing the time taken to perform a single measurement using the claimed device.

The invention relates to the technical field of photonic crystal materials and lactic acid analysis and detection, in particular to a preparation method and application of a photonic crystal lactic acid sensor. The photonic crystal lactic acid sensor comprises a lactic acid responsive hydrogel matrix membrane and a plurality of uniformly oriented one-dimensional magnetic nano chains embedded in the lactic acid responsive hydrogel matrix membrane; the one-dimensional magnetic nano chain is formed by arranging monodispersed magnetic nano particles in a non-close packing one-dimensional chain shape. According to the photonic crystal lactic acid sensor disclosed by the invention, in a visible spectrum range, the maximum value of the sensitivity is greater than or equal to 11.5 nm / mM, and the response range of the photonic crystal lactic acid sensor is an interval of lactic acid concentration from 0 to 50mM. The photonic crystal lactic acid sensor disclosed by the invention can distinguish the concentration change of the lactic acid which can reach 0.87 mM in a to-be-detected liquid only by recognizing the color change of the sensor by human eyes in a specific environment, is high in visualization degree and can be recycled for multiple times.

The present invention relates to a method for expanding a population of lymphocytes in a single, controlled culture vessel, comprising: (a) culturing a tissue or blood sample from a subject, the sample being known or suspected to contain lymphocytes; or (b) culturing lymphocytes, the lymphocytes being lymphocytes isolated from a tissue or blood sample from the subject; wherein the lymphocytes are expanded in a culture medium in which at least one of the following parameters is monitored and adjusted to a predefined value or range: pH, dissolved oxygen (DO) concentration, glucose concentration, lactate concentration, and / or temperature; and wherein the method comprises adjusting the culture volume to match the rate of lymphocyte expansion. Furthermore, the present invention relates to a population of lymphocytes comprising at least 90% CD3+ T cells and less than 5% B cells, wherein at least 70% of the T cell portion is viable and, optionally, less than 10% are triple positive for CD45RA, CD57, and KLRG1.

The invention discloses a lactic acid biosensor and a preparation method thereof.The lactic acid biosensor comprises a substrate, a working electrode, a reference electrode, a counter electrode, a lactic acid active enzyme layer and an outer membrane adjusting layer, the working electrode and the reference electrode are distributed on one face of the substrate, and the counter electrode is distributed on the other opposite face of the substrate; the lactic acid active enzyme layer is arranged on the surface of the working electrode, and the working electrode, the reference electrode and the counter electrode are completely wrapped by the outer membrane adjusting layer. The lactic acid sensor provided by the invention has the characteristics of high sensitivity, safe use process, stable performance and process, sustainable use and the like, can continuously monitor lactic acid for 14 days without signal attenuation through tests, and can effectively and quickly identify the change of the lactic acid concentration in vivo.

This invention discloses a genetically engineered bacterium and its application in enhancing lactic acid production in *Escherichia coli*, belonging to the field of bioengineering. The genetically engineered bacterium of this invention uses *E. coli* as the starting strain and is obtained through genetic engineering operations. In this genetically engineered bacterium, the phosphoacetyltransferase-acetylkinase locus pta-ack and the alcohol dehydrogenase gene adhE are knocked out or deleted, while exogenous lactate dehydrogenase and exogenous monocarboxylic acid transporter genes are introduced and overexpressed. The modified genetically engineered bacterium, while retaining the advantages of *E. coli* such as easy culture and rapid growth rate, significantly improves the yield and conversion rate of lactic acid. Compared to the starting strain, the lactic acid concentration increases to 6 g / L with pta-ack knockout alone, and to 8 g / L with double knockout of pta-ack and adhE. Furthermore, the lactic acid concentration increases to approximately 20 g / L and 23 g / L with overexpression of exogenous lactate dehydrogenase and exogenous monocarboxylic acid transporter, respectively.

The present invention relates to a method for expansion of a population of lymphocytes in a controlled single culture vessel, the method comprising a step of (a) culturing a tissue or blood sample from a subject, which sample is known or suspected to contain lymphocytes; or (b) culturing lymphocytes, which lymphocytes are isolated from a tissue or blood sample from a subject; wherein the lymphocytes are expanded in a culture medium in which at least one of the following parameters is monitored and adjusted to a predefined value or range: pH, dissolved oxygen (DO) concentration, glucose concentration, lactate concentration and / or temperature; and wherein the method comprises a step of adjusting the culture volume to the expansion rate of the lymphocytes. Furthermore, the invention relates to a population of lymphocytes comprising at least 90% CD3+ T cells and less than 5% B cells, wherein at least 70% of said T cell portion are viable, optionally, and less than 10% are triple positive for CD45RA, CD57 and KLRG1.

The invention relates to an agent for application to skin areas of a human for increasing the power of muscles of the human under the skin areas in the event of anaerobic stress, in particular for reducing a lactate concentration in muscles under the skin areas. The agent expediently has a beta-alanine content of between 5.00 vol.% and 20.00 vol.% and a water content of between 20.00 vol.% and 95.00 vol.%. Overall, the claimed content of beta-alanine leads to a low-side-effect increase in power of those muscles that lie below skin areas treated with an agent according to the invention. In one embodiment of the invention, the agent additionally comprises water-soluble magnesium salt, wherein a content of the water-soluble magnesium salt is between 3.00 vol.% and 6.00 vol.%, and wherein the water-soluble magnesium salt is selected from the group consisting of magnesium chloride, magnesium sulfate, magnesium acetate, magnesium citrate, magnesium glycinate and magnesium gluconate.

The invention relates to the technical field of feedback regulation, in particular to an electric field stimulation feedback regulation system based on organoid micro-reaction observation, which comprises the following steps: acquiring an electrical stimulation time period between two adjacent electrical stimulation parameter regulation moments, and determining fatigue influence sensitivity according to the numerical change of electrical stimulation parameters and morphological similarity analysis of action potentials; according to the lactic acid concentration distribution and the change of the action potential amplitude, determining the fatigue superposition expression degree of the similar organs in each electrical stimulation period, and obtaining a fatigue early warning coefficient by combining the fatigue superposition expression degree of the current electrical stimulation period and the fatigue influence sensitivity of the current period set; and according to the fatigue early warning coefficient of the current electrical stimulation period, performing adjustment early warning on the electrical field stimulation. According to the method, the fatigue state can be accurately judged in combination with the change and superposition expression of the electrical stimulation parameters, fatigue early warning is carried out in time, and the possibility of irreversible damage of the organoids caused by continuous electrical stimulation is reduced.

The present application relates to photonic crystal materials and lactic acid analysis detection technical field, particularly to a kind of preparation method and application of photonic crystal lactic acid sensor, including lactic acid responsive hydrogel matrix film and embedding therein uniform orientation's multiple one-dimensional magnetic nanochain, the one-dimensional magnetic nanochain is by monodisperse magnetic nanoparticle with non-tightly packed one-dimensional chain arrangement.The maximum value that the sensitivity of photonic crystal lactic acid sensor of the present application can reach in visible spectral range is >=11.5nm / mM, and the response range of the photonic crystal lactic acid sensor is the interval that lactic acid concentration is from 0 to 50mM.The photonic crystal lactic acid sensor of the present application can distinguish the minimum lactic acid concentration change of 0.87mM in the liquid to be measured in specific environment only by relying on human eye to identify sensor color change, and the degree of visualization is high, and can be used repeatedly.

The present invention relates to a composition for improving muscle function and a functional health food, which, by containing sialyllactose as an active ingredient, reduce the blood lactic acid concentration and increase the expression of a gene related to muscle function improvement, thereby improving muscle function without excessively increasing the lactic acid concentration as a fatigue substance. The fatigue resistance, the muscular endurance and the exercise performance of muscles are improved.

The present application relates to the technical field of feedback regulation, and particularly relates to an electric field stimulation feedback regulation system based on organoid micro-reaction observation, comprising: obtaining an electric stimulation period between two adjacent electric stimulation parameter adjustment time points, determining fatigue influence sensitivity according to the numerical change of the electric stimulation parameter and the morphological similarity analysis of the action potential; determining the fatigue superposition performance degree of the organoid in each electric stimulation period according to the lactic acid concentration distribution and the change of the action potential amplitude, combining the fatigue superposition performance degree of the current electric stimulation period and the fatigue influence sensitivity of the time period set to obtain a fatigue early warning coefficient; and adjusting and warning the electric field stimulation according to the fatigue early warning coefficient of the current electric stimulation period. The present application can accurately judge the fatigue state and timely perform fatigue early warning by combining the change of the electric stimulation parameter and the superposition performance, thereby reducing the possibility of irreversible damage of the organoid caused by continuous electric stimulation.

The utility model relates to the technical field of calcium lactate concentration, in particular to a calcium lactate two-stage forced circulation evaporator, which preheats a pipeline and liquid through a preheating device, improves the initial temperature of calcium lactate, reduces the heating time of the calcium lactate in an evaporation container, and heats and evaporates the calcium lactate through a heating device. The concentrated calcium lactate is sent out of the equipment at a constant speed through the extrusion device, so that the practicability and the stability of the equipment are improved; comprising a preheating device, a heating device and an extrusion device, the heating device is connected with the preheating device, and the extrusion device is connected with the heating device.