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D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration

A kit, lactic acid technology, applied in the field of medical/food testing and determination, can solve problems such as microbial contamination

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If D-lactic acid is present, microbial contamination may be the cause

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0100] The D-lactic acid diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0101] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0102] Stabilizer 500mmol / L

[0103] Reduced coenzyme 0.25mmol / L

[0104] 2 Ferricytochrome c 10mmol / L

[0105] Ammonia ion 20mmol / L

[0106] D-lactate dehydrogenase 10000U / L

[0107] Alanine dehydrogenase 10000U / L

[0108] Glycine oxidase 10000U / L

[0109] Peroxidase 30000U / L

[0110] 4-amino antiarsenic 2mmol / L

[0111] Carbolic acid 10mmol / L

[0112] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0113] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 505nm, test sub-wavelength 600nm, the volume ratio of the measured D-lactic acid sample to the reagent is 1 / 25, and the reaction direction is...

Embodiment 2

[0116] The D-lactic acid diagnosis / measurement reagent of this embodiment is a double reagent, including:

[0117] Reagent 1

[0118] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0119] Stabilizer 50mmol / L

[0120] Reduced coenzyme 0.25mmol / L

[0121] 2 Ferricytochrome c 10mmol / L

[0122] Ammonia ion 20mmol / L

[0123] 2,4,6-tribromo-3-hydroxy-benzenesulfonic acid 0.2mmol / L

[0124] Reagent 2

[0125] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0126] Stabilizer 500mmol / L

[0127] D-lactate dehydrogenase 10000U / L

[0128] Alanine dehydrogenase 10000U / L

[0129] Glycine oxidase 10000U / L

[0130] Peroxidase 25000U / L

[0131] 4-amino antiarsenic 0.6mmol / L

[0132] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0133] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 54...

Embodiment 3

[0136] The D-lactic acid diagnosis / measurement reagent of the present embodiment is three reagents, comprising:

[0137] Reagent 1

[0138] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0139] Stabilizer 50mmol / L

[0140] Reduced coenzyme 0.25mmol / L

[0141] 2 Ferricytochrome c 10mmol / L

[0142] Ammonia ion 20mmol / L

[0143] 3-methyl-2-benzothiazolone hydrazone 0.6mmol / L

[0144] Reagent 2

[0145] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0146] Stabilizer 500mmol / L

[0147] Alanine dehydrogenase 10000U / L

[0148] Glycine oxidase 10000U / L

[0149] Peroxidase 20000U / L

[0150] Dimethylaniline 2mmol / L

[0151] Reagent 3

[0152] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0153]Stabilizer 500mmol / L

[0154] D-lactate dehydrogenase 10000U / L

[0155] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

...

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Abstract

The invention relates to a kit for diagnosing / mensurating D-lactic acid by utilizing the technologies of the enzymatic cycling amplification method, the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA) based on the specificity of the D-lactic acid. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the D-lactic acid, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, 2-ferricytochrome-c, ammonia ions, D-lactate dehydrogenase, alanine dehydrogenase, glycine oxidase, peroxidase, a reduced chromogen combination and a stabilizer. Through a series of enzymatic reactions, the colorless reduced chromogen combination is finally oxidized into colored dyes, thereby mensurating the content of the dyes at 400nm-700nm of the wavelength by utilizing a visible light analyzer and further reflecting the concentration of the D-lactic acid directly.

Description

technical field [0001] The invention relates to a D-lactic acid diagnosis / measurement kit with D-lactic acid specificity, and at the same time, the invention also relates to a method for measuring D-lactic acid concentration, which belongs to the technical field of medical / food inspection and determination. Background technique [0002] L-lactic acid is an organic acid that has attracted much attention and developed rapidly in recent years. At the same time, L-lactic acid has application prospects in many fields such as food, feed, medicine, plastics, feed, pesticide, daily chemical industry, papermaking and electronic industry. [0003] L-lactic acid is the end product of the metabolism of most organisms. Only a few microorganisms (such as Lactobacillus, Leuconostoc) can form D-lactic acid. Great care is taken in the production of yogurt to detect lactate to assess whether the microbes are producing L-lactic acid, D-lactic acid or both. If D-lactic acid is present, micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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