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Diagnostic reagent (kit) of adenosine deaminase and method for measuring activity concentration of adenosine deaminase

An adenosine deaminase and reagent technology, which is applied in biochemical equipment and methods, microbial determination/inspection, color/spectral property measurement, etc., and can solve problems such as determination and difficult popularization and application

Inactive Publication Date: 2010-06-23
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot be determined by the visible light analyzer in general hospitals, but requires the use of a special ultraviolet light analyzer, so it is difficult to practically popularize and apply

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The adenosine deaminase diagnostic reagent of this embodiment is a single reagent, comprising:

[0042] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0043] Stabilizer 500mmol / L

[0044] Reduced coenzyme 0.25mmol / L

[0045] Nitrite reductase 10000U / L

[0046] Nitrite reductase 10000U / L

[0047] Adenosine 5mmol / L

[0048] Ferricytochrome C 5mmol / L

[0049] Calcium chloride 3mmol / L

[0050] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0051] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the tested adenosine deaminase sample to the reagent is 1 / 25, the reaction direction is negative reaction (falling reaction), the delay time is about 1 minute, the detection...

Embodiment 2

[0054] The adenosine deaminase diagnostic reagent of this embodiment is a double reagent, comprising:

[0055] Reagent 1

[0056] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0057] Stabilizer 50mmol / L

[0058] Reduced coenzyme 0.25mmol / L

[0059] Adenosine 5mmol / L

[0060] Ferricytochrome C 5mmol / L

[0061] Calcium chloride 3mmol / L

[0062] Reagent 2

[0063] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0064] Stabilizer 500mmol / L

[0065] Nitrite reductase 10000U / L

[0066] Nitrite reductase 10000U / L

[0067] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0068] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, tested adenosine deaminase sample and reagent 1, reagent 2 The volume ratio is 2 / 20 / 5, the ...

Embodiment 3

[0071] The adenosine deaminase diagnostic reagent of this embodiment is three reagents, comprising:

[0072] Reagent 1

[0073] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0074] Stabilizer 50mmol / L

[0075] Reduced coenzyme 0.25mmol / L

[0076] Adenosine 5mmol / L

[0077] Ferricytochrome C 5mmol / L

[0078] Calcium chloride 3mmol / L

[0079] Reagent 2

[0080] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0081] Stabilizer 500mmol / L

[0082] Nitrite reductase 10000U / L

[0083] Reagent 3

[0084] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0085] Stabilizer 500mmol / L

[0086] Nitrite reductase 10000U / L

[0087] After all the reagents are dissolved and prepared, they are divided into bottles to make liquid three reagents, which can be used directly.

[0088] When measuring the activity concentration of adenosine deaminase, set on the automatic biochemical analyzer: temperature 37°C, reaction time ...

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PUM

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Abstract

The invention relates to a diagnostic reagent (kit) of adenosine deaminase, which utilizes an enzyme circulation multiplication method, enzyme colorimetry and an enzyme linkage method technology. Meanwhile, the invention also relates to a method for measuring the activity concentration of the adenosine deaminase and the formation and the components of the reagent, which belong to the technical fields of the inspection and the measurement of medicine. The reagent (kit) comprises the main components of a buffer solution, reduced coenzyme, adenosine, ferricytochrome C, calcium chloride, two different nitrite reductases and a stabilizing agent; and a sample and the reagent generate a series of enzymatic reactions by mixing the sample and the reagent according to a certain volume ratio, then a reactant is put under an ultraviolet / visible light analyzer, and the descending speed of the absorbency of a position with the dominant wavelength of 340nm is detected, thereby measuring and calculating the magnitude of the activity concentration of the adenosine deaminase.

Description

technical field [0001] The invention relates to a diagnostic reagent (box) for adenosine deaminase, and at the same time, the invention also relates to a method for measuring the activity concentration of adenosine deaminase, which belongs to the technical field of medical examination and measurement. Background technique [0002] Medical research shows that adenosine deaminase is a nucleic acid metabolizing enzyme that has an important relationship with the body's cellular immune activity. Therefore, the determination of adenosine deaminase activity is used as an important diagnostic marker for the differential diagnosis of benign and malignant hydrothorax, cerebrospinal fluid, severe combined immunodeficiency disease (SCID), acute and chronic hepatitis, liver cirrhosis, liver cancer and other diseases. [0003] The methods for determining the activity of adenosine deaminase mainly include radionuclide method, physical method and biochemical method. As far as the applicant...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/17G01N33/48C12Q1/26
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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