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D-lactic acid diagnosis/determination reagent kit and method for determining D-lactic acid concentration

A kit, lactic acid technology, applied in the field of medical/food testing and determination, can solve problems such as microbial contamination

Inactive Publication Date: 2008-12-17
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If D-lactic acid is present, microbial contamination may be the cause

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The D-lactic acid diagnostic / measurement reagent in this embodiment is a single reagent, including:

[0043] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0044] Stabilizer 500mmol / L

[0045] Reduced coenzyme 0.25mmol / L

[0046] 2 high iron cytochrome c 10mmol / L

[0047] Carbon dioxide 20mmol / L

[0048] Lactate dehydrogenase 10000U / L

[0049] Malate dehydrogenase 16000U / L

[0050] After all the reagents are dissolved and prepared, they are divided into bottles and freeze-dried to prepare dry powder reagents; before use, add pure water and use after reconstitution.

[0051] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, the volume ratio of the tested D-lactic acid sample to reagent is 1 / 25. The reaction direction is negative reaction (declining reaction), the delay time is about 0 minutes, and the detection time is about 5 minutes....

Embodiment 2

[0054] The D-lactic acid diagnostic / measurement reagent in this embodiment is a dual reagent, including:

[0055] Reagent 1

[0056] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0057] Stabilizer 50mmol / L

[0058] Reduced coenzyme 0.25mmol / L

[0059] 2 high iron cytochrome c 10mmol / L

[0060] Carbon dioxide 20mmol / L

[0061] Reagent 2

[0062] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0063] Stabilizer 500mmol / L

[0064]Lactate dehydrogenase 10000U / L

[0065] Malate dehydrogenase 16000U / L

[0066] After all the reagents are dissolved and prepared, they are divided into bottles to form a liquid double reagent, which can be used directly.

[0067] Set on the automatic biochemical analyzer: temperature 37℃, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test sub-wavelength 405nm, volume of D-lactic acid sample and reagent 1, reagent 2 The ratio is 2 / 20 / 5, the reaction direction is negative reaction ...

Embodiment 3

[0070] The D-lactic acid diagnostic / determination reagent in this embodiment is three reagents, including:

[0071] Reagent 1

[0072] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0073] Stabilizer 50mmol / L

[0074] Reduced coenzyme 0.25mmol / L

[0075] 2 high iron cytochrome c 10mmol / L

[0076] Carbon dioxide 20mmol / L

[0077] Reagent 2

[0078] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0079] Stabilizer 500mmol / L

[0080] Malate dehydrogenase 16000U / L

[0081] Reagent 3

[0082] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0083] Stabilizer 500mmol / L

[0084] Lactate dehydrogenase 10000U / L

[0085] After all the reagents are dissolved and prepared, they are divided into bottles to form three liquid reagents, which can be used directly.

[0086] When determining the concentration of D-lactic acid, set it on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1....

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Abstract

The invention relates to a kit for diagnosing / mensurating D-lactic acid by utilizing the technologies of the enzymic colorimetric method and the enzyme linked immunosorbent assay (ELISA) based on the specificity of the D-lactic acid. The invention further relates to a method, a principle and the composition and the components of a reagent for mensurating the concentration of the D-lactic acid, and belongs to the technology field of medical / food inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, 2-ferricytochrome-c, carbon dioxide, lactate dehydrogenase, malate dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the degree / velocity of the decrease in absorbance at 340nm of the dominant wavelength is detected, thereby mensurating the concentration of the D-lactic acid.

Description

Technical field [0001] The invention relates to a D-lactic acid diagnosis / determination kit with D-lactic acid specificity. At the same time, the invention also relates to a method for measuring the concentration of D-lactic acid, which belongs to the technical field of medical / food inspection and determination. Background technique [0002] L-lactic acid is an organic acid that people pay close attention to and develop rapidly in recent years. At the same time, L-lactic acid has application prospects in many fields such as food, feed, medicine, plastics, feed, pesticides, daily chemical industry, papermaking and electronics industry. [0003] L-lactic acid is the end product of metabolism in most organisms. Only a few microorganisms (such as Lactobacillus and Leuconostoc) can form D-lactic acid. Pay great attention to the detection of lactate when producing yogurt to assess whether the microorganisms produce L-lactic acid, D-lactic acid or both. If D-lactic acid is present, it m...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N21/31C12Q1/26C12Q1/00
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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