Slow-release antibacterial acellular matrix biological material and preparation method thereof

An acellular matrix and biomaterial technology, which is applied in the field of slow-release antibacterial acellular matrix biomaterials and their preparation, can solve problems such as unfavorable early vascularization, and achieves improved biocompatibility, increased loading, and stable release rate. control effect

Pending Publication Date: 2021-08-20
浙江德普斯医疗科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, compared with non-crosslinked materials, the acellular matrix biomaterial obtained by this method significantly increased the infection rate of the repair area when applied to the contaminated surface, which ...

Method used

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  • Slow-release antibacterial acellular matrix biological material and preparation method thereof
  • Slow-release antibacterial acellular matrix biological material and preparation method thereof
  • Slow-release antibacterial acellular matrix biological material and preparation method thereof

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preparation example Construction

[0052] The slow-release antibacterial acellular matrix biomaterial and its preparation method according to the embodiment of the present invention comprise the following steps:

[0053] Step S1, providing a biological tissue, and performing decellularization and virus inactivation on the biological tissue.

[0054] That is to say, after the biological tissue is obtained, the biological tissue should first be decellularized and virus inactivated.

[0055] Among them, the biological tissue is not particularly limited, and may be, for example, mammalian-derived hollow organ submucosa, tissue basement membrane, dermis, pericardium, peritoneum, pleura, or amnion.

[0056] After the biological tissue is obtained, the biological tissue needs to be decellularized and virus inactivated.

[0057] Wherein, decellularization can be performed by any one of physical methods, chemical methods, and biological methods.

[0058] Wherein, the physical method of decellularization mainly involve...

Embodiment approach

[0059] As an embodiment, the decellularization process of the biological tissue is as follows: the biological tissue is treated by repeated freezing and thawing, and then treated with 1M hypertonic saline for 2 hours and 0.2% Triton X-100 for 2 hours in a constant temperature water bath shaker , 0.4% NaOH treatment for 2h. Wherein, the oscillation rate of the constant temperature water bath oscillator can be, for example, 120 rpm, and the temperature of the water bath is 37°C.

[0060] In addition, the virus inactivation treatment can be performed by a virus inactivation reagent, and the virus inactivation reagent is one or more of peracetic acid, 75% alcohol, and sodium hydroxide.

[0061] As an embodiment, the virus inactivation process of the biological tissue is: the biological tissue after the decellularization treatment is treated with 0.1% peracetic acid (PAA) for 2 hours in a constant temperature water bath shaker, and / or with 75% peracetic acid Fully soak in alcohol ...

Embodiment 1

[0106] 1. Preparation of slow-release antibacterial acellular matrix biomaterials

[0107] (A) Biological tissue and its processing

[0108] Step 1: Dissection of porcine bladder matrix

[0109] 1) After washing the purchased fresh pig bladders, they are quickly frozen at a temperature of -20 to -40°C, and the freezing time is not less than 24 hours.

[0110] Take out the frozen porcine bladder, soak it in tap water at normal temperature to thaw, and soak it for 1 to 2 hours.

[0111] Then rinse it inside and out with tap water.

[0112] 2) Separate the fat and fascia around the bladder.

[0113] 3) Insert one end of the rubber catheter along the urethral opening of the bladder and fix it with a cable tie. The other end of the catheter is connected to the faucet, and water is slowly injected into the bladder through the catheter. The volume of water is about 700-1200 mL until the bladder is fully inflated and the blood vessels are clear.

[0114] 4) Separate the porcine bl...

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Abstract

The invention provides a slow-release antibacterial acellular matrix biological material and a preparation method thereof. The preparation method comprises the following steps: S1, providing a biological tissue, and carrying out decellularization and virus inactivation treatment on the biological tissue; S2, performing plasma treatment on the treated biological tissue to modify the surface of the biological tissue to obtain an acellular matrix; S3, soaking the acellular matrix in a slow-release antibacterial aqueous solution containing an organic slow-release medium, an antibacterial agent and weak acid, so that the organic slow-release medium is loaded on the surface of the acellular matrix, the antibacterial agent is loaded on the surface of the organic slow-release medium, the weak acid is dilute acetic acid, dilute hydrochloric acid or dilute lactic acid, and the concentration of the weak acid is 0.01-1 mol/L; and S4, taking out the soaked acellular matrix, and performing vacuum freeze drying and sterilization to obtain the slow-release antibacterial acellular matrix biological material.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a slow-release antibacterial acellular matrix biological material and a preparation method thereof. Background technique [0002] The study of acellular matrix biomaterials is an important breakthrough in the field of tissue function. Compared with traditional non-absorbable polymer materials, acellular matrix biomaterials act as temporary tissue scaffolds after implantation. With the degradation of patch materials, the host Cells infiltrate it and produce autologous collagen fibers. Such an endogenous tissue remodeling process can achieve early local rapid revascularization, early entry of phagocytes, and difficulty in the formation of bacterial biofilms, so it has a certain resistance to infection. [0003] However, it is undeniable that acellular matrix biomaterials are only partially resistant to infection rather than anti-infection, and have no antimicrobial activity th...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/54A61L27/58
CPCA61L27/3604A61L27/3691A61L27/3687A61L27/54A61L27/58A61L2300/604A61L2300/404
Inventor 郭瀛军吕国忠王健沛陈晓璐唐晓钰叶海雁刘抗
Owner 浙江德普斯医疗科技股份有限公司
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