The invention relates to cordyceps beverage with the function of anti-fatigue and a preparation method thereof. The beverage mainly contains 15-30 percent of cordyceps fermentation liquid, 1-10 percent of extracted rhodiola, 0.1-1.5 percent of medlar polysaccharide powder, 0.5-3.0 percent of honey, 5-15 percent of sucrose, 0.1-1.0 percent of sea buckthorn fruit powder, 0.01-0.2 percent of flavoring and the rest is purified water. The cordyceps beverage with the function of anti-fatigue has the advantages of specific flavor and the performances of anti-fatigue, hypoxia resistance and immunity improvement. At the same time, the beverage can improve the short time memory of moderate plateau people and the level of partial pressure of oxygen in blood, lower the frontload and the afterload of hearts, improve the function of hearts, increase the content of oxygen in blood effectively, reduce the lactic acid level in blood, lead healthy men to work efficiently and permanently with abundant spirit, enhance the function of heart and cardiovascular and improve sexual function. And the beverage is especially suitable for the crowds working in front of computers for a long time.