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Lactate sensor

A sensor, lactic acid technology, applied in the field of lactic acid sensor, can solve the problems of slow enzyme reaction speed, shortened measurement time, long time, etc., and achieve the effect of excellent precision

Active Publication Date: 2013-04-03
ARKRAY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, conventional lactic acid sensors require a long time for measurement due to the slow enzyme reaction rate, and further shortening of the measurement time is required.

Method used

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Effect test

Embodiment approach

[0071] [1] A lactic acid sensor comprising: an insulating substrate, an electrode system including at least a working electrode and a counter electrode disposed on the substrate, and a reagent layer disposed on the electrode system,

[0072] The reagent layer contains lactate oxidase, mediator, and N-(2-acetamido)-2-aminoethanesulfonic acid.

[0073] [2] The lactic acid sensor according to [1], wherein the reagent layer further contains a sugar selected from the group consisting of oligosaccharides and polysaccharides.

[0074] [3] The lactic acid sensor according to [2], wherein the sugar is dextran or maltose, preferably dextran.

[0075] [4] The lactic acid sensor according to any one of [1] to [3], wherein the reagent layer further contains a layered inorganic compound and / or a surfactant.

[0076] [5] The lactic acid sensor as described in any one of [1] to [4], wherein the mediator is selected from potassium ferricyanide, cytochrome C, pyrroloquinoline quinone, NAD + 、...

Embodiment 1

[0096] Produced as shown below with figure 1 The lactate sensor shown has the same structure as the lactate sensor of Example 1.

[0097] First, as the insulating substrate 11 of the lactic acid sensor, a PET substrate (50 mm in length, 6 mm in width, and 250 m in thickness) was prepared, and on one surface thereof, a working electrode 12 having lead portions 12 a and 13 a were formed by screen printing. And the carbon electrode system that the counter electrode 13 constitutes.

[0098] Then, the insulating layer 14 is formed on the electrode system as follows. First, an insulating resin polyester was dissolved in a solvent carbitol acetate to have a concentration of 75% by weight to prepare an insulating paste, which was screen printed on the electrode system. The printing conditions are: 300-mesh screen, 40kg scraper pressure, and the printing volume is per 1cm 2 The electrode area is set to 0.002mL. In addition, screen printing is not performed on the detection part 15 ...

Embodiment 2

[0109] A lactic acid sensor was fabricated in the same manner as in Example 1, except that the second reagent solution contained 0.5% by weight of dextran (molecular weight: 5000-10000, trade name "Dextran", Nacalai Tesque). Using the lactic acid sensors of Examples 1 and 2, venous whole blood with adjusted lactic acid concentration (lactate concentration: 4, 6, 10, 18, 24 mg / dL) was used as a sample, and the measurement temperature conditions were set at 25°C or 10°C , except that, the measurement of lactic acid was carried out in the same manner as in Example 1. express the result in Figure 4 middle. Figure 4 A is a graph of the measured temperature condition of 25°C, Figure 4 B is a graph where the measurement temperature condition is 10°C. Figure 4 In A and B, square marks represent the graph of the lactic acid sensor of Example 1, and rhombus marks represent the graph of the lactic acid sensor of Example 2.

[0110] It was confirmed that both of the lactic acid se...

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Abstract

The invention relates to a lactate sensor, and specifically a lactate sensor capable of accurately measuring a lactate concentration in a short period of time. The lactate sensor includes an insulating substrate, an electrode system including at least a working electrode and a counter electrode provided on the substrate, and a reagent layer provided on the electrode system. The reagent layer contains lactate oxidase, a mediator, and N-(2-acetamide)-2-aminoethanesulfonic acid.

Description

technical field [0001] The present disclosure relates to a lactate sensor containing lactate oxidase and a mediator. Background technique [0002] Lactic acid is known to be a fatigue substance produced in the body after exercise. Therefore, in order to grasp the training effect and training state, lactic acid sensors are widely used in arenas or in the field, and it is also required to be able to detect and measure them easily. However, since the lactate oxidase used in the lactate sensor has a higher unit price per unit than the glucose oxidase used in the glucose sensor, there is a problem that the unit price of the sensor increases. Therefore, in order to solve this problem, for example, a lactate sensor including phosphate in a reaction layer to increase the activity of lactate oxidase has been proposed (Japanese Patent No. 3498105). [0003] In addition, as a method of manufacturing a biosensor such as a lactic acid sensor, for example, there is a method including a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/3272C12Q1/004C12Q1/005C12Q1/26G01N2333/904
Inventor 兼田悠山口武广
Owner ARKRAY INC
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