Full blood lactic biological sensor modified by oriented carbon nano tube

A biosensor, carbon nanotube technology, applied in the field of biosensors, can solve the problems of interference of changes in oxygen content, poor redox properties, poor anti-interference ability, etc., to improve the activity of enzymes, improve the response sensitivity, avoid Effects of changes in blood volume

Inactive Publication Date: 2009-04-29
ZHEJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The defect of this sensor is: the voltage added to the electrode leads is relatively high, which is 0.5v, and the anti-interference ability is not good. better response current
Chinese patent CN1219676A introduces a disposable dual-electron mediator dual-enzyme biosensor for detecting cholesterol, glucose, etc. in the blood, but this sensor requires the participation of oxygen and is easily disturbed by changes in oxygen content in the blood

Method used

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  • Full blood lactic biological sensor modified by oriented carbon nano tube
  • Full blood lactic biological sensor modified by oriented carbon nano tube
  • Full blood lactic biological sensor modified by oriented carbon nano tube

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Effect test

Embodiment 1

[0011] A whole blood lactic acid biosensor modified by oriented carbon nanotubes, the bottom plate 1 is anodized to form Al 2 o 3 The aluminum plate of the insulating layer, and the electrochemical method is used in the Al 2 o 3 Co particles that act as catalysts for the growth of oriented carbon nanotubes are deposited in the longitudinal micropores of the insulating layer; the electrode 2 is an Au electrode formed on the bottom plate 1 by vacuum sputtering, and the cavity 3 is covered with a plastic film around the electrode 2 Enclosed, a capillary hole 3a for sampling is opened on one of its side walls, the reaction layer 4 is arranged in the cavity 3, and its bottom layer 4a is an aligned carbon nanotube layer used as a second electron mediator. The carbon nanotube layer is obtained by chemical vapor deposition, the directional growth time is 5 minutes, and the ultrasonic cut-off time is 4 hours. The upper layer 4b is dripped with L-lactate oxidase and reagents, stabiliz...

Embodiment 2

[0013] A whole blood lactic acid biosensor modified by oriented carbon nanotubes, the bottom plate 1 is formed by anodic oxidation on its surface to form Al 2 o 3 The aluminum plate of the insulating layer, and the electrochemical method is used in the Al 2 o 3 Co particles that act as catalysts for the growth of oriented carbon nanotubes are deposited in the longitudinal micropores of the insulating layer; the electrode 2 is an Au electrode formed on the bottom plate 1 by vacuum sputtering, and the cavity 3 is covered with a plastic film around the electrode 2 Enclosed, a capillary hole 3a for sampling is opened on one of its side walls, the reaction layer 4 is arranged in the cavity 3, and its bottom layer 4a is an aligned carbon nanotube layer used as a second electron mediator. The carbon nanotube layer is obtained by chemical vapor deposition, the directional growth time is 5 minutes, and the ultrasonic cutting time is 8 hours. The upper layer 4b is dripped with L-lacta...

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Abstract

The full blood lactic acid biosensor modified with oriented carbon nanotube features that one layer of Co particles as the catalyst for oriented growth of carbon nanotube is deposited on the substrate and that the reaction layer has base layer comprising oriented carbon nanotube as the second electric intermediate and the upper layer comprising L-lactic oxidase and the mixture of reagent, stabilizer and buffering liquid as the first electric intermediate. Compared with available the technology, the present invention has the advantages of obviously raised responding sensitivity on lactic acid concentration of the sensor, work voltage of the sensor lowered from 500 mv to 300 mv, no need of pre-treatment of the blood sample and blood sample amount as less as 2 microliter.

Description

technical field [0001] The invention relates to a biosensor, in particular to a whole blood lactic acid biosensor modified by directional carbon nanotubes. Background technique [0002] Whole blood lactic acid biosensor modified by oriented carbon nanotubes is a biosensor that uses biomolecules as sensitive elements to quickly, accurately and conveniently measure lactic acid content in blood and other liquids. This sensor is used to measure lactic acid content in human blood. It is used to reveal the severity of certain diseases, such as guiding diabetic patients to take Jiangtangling appropriately. Excessive use of Jiangtangling will cause glycolysis, increase the lactic acid content, cause acidosis, and endanger the patient's life; it is used to measure the concentration of lactic acid in athletes, It can scientifically control the training intensity of athletes to achieve the best training effect; in food science, the freshness of food can be determined by detecting the c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/403G01N27/327G01N27/26
Inventor 陈裕泉潘敏陈文菊陈金霞
Owner ZHEJIANG UNIV
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