Enterococcus faecalis strain and application thereof

A technology for Enterococcus faecalis and strains, applied in the field of Enterococcus faecalis, can solve the problem of low final concentration of lactic acid produced by bacterial fermentation, and achieve the effects of small product inhibition, high lactic acid concentration and lower fermentation cost.

Active Publication Date: 2020-05-05
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the lactic acid production ability of the strain has been improved by ultraviolet mutagenesis, the final concentration of lactic acid produced by the strain is still low

Method used

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  • Enterococcus faecalis strain and application thereof
  • Enterococcus faecalis strain and application thereof
  • Enterococcus faecalis strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Enterococcus faecalis ( Enterococcus faecalis ) DLY-LFYC genetic stability test.

[0031] Using MRS medium, inoculate the obtained strains on the plate for five generations continuously, pick the colonies on each generation plate with an inoculation loop and transfer them to the seed culture solution for shake flask culture. The culture conditions are: temperature 45°C, rotation speed 150r / min , the culture time is 12h, and then each generation is fermented in a 5L fermenter with 2L liquid fermentation medium inside with 10% inoculum. The fermentation conditions are: temperature 45°C, speed 150r / min, pH 7.0 Sodium hydroxide was used as a neutralizing agent, and the fermentation time was 36 hours. During the fermentation process, glucose was added to make the total concentration of glucose in the fermentation medium reach 170 g / L. The fermentation results are shown in Table 2.

[0032] Table 2 Fermentation results of strain genetic stability

[0033]

Embodiment 2

[0034] Example 2 Lactose concentration and optical purity of different carbon sources

[0035] Using MRS medium, the difference is that glucose is replaced by other kinds of carbon sources. The results are shown in Table 3.

[0036] Table 3 Fermentation results of different carbon sources

[0037]

Embodiment 3

[0038] Example 3 Cellulose hydrolyzate cellobiose as fermentation substrate

[0039] Using MRS medium, the difference is that cellobiose, a cellulose hydrolyzate, is used as a substrate to ferment and produce L-lactic acid. The concentration of L-lactic acid is higher than 50g / L, and the optical purity of L-lactic acid is higher than 99.6%. Cellobiose is converted to lactic acid.

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Abstract

The invention relates to an enterococcus faecalis strain for producing L-lactic acid and an application of the enterococcus faecalis strain. The enterococcus faecalis strain is classified and named asEnterococcus faecalis, is preserved in China General Microbiological Culture Collection Center on May 28,2018, and has a preservation number of CGMCC No.15821. The strain has high yield of L-lactic acid, can utilize a plurality of carbon sources, and has a little product inhibition effect. The lactic acid concentration is high, and particularly, optical purity of the L-lactic acid can reach 99 percent or above.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Enterococcus faecalis producing L-lactic acid and its application. Background technique [0002] Lactic acid is one of the three major organic acids recognized in the world. It has optical isomers and is divided into D-lactic acid, L-lactic acid and DL-lactic acid. Among them, L-lactic acid is L-lactic acid, which is the only one that can be decomposed and utilized by the human body. Lactic acid type. L-lactic acid is widely used in food, medicine, agriculture, cosmetics and many other fields, especially L-lactic acid with high optical purity, because it can be used as the precursor of biodegradable material L-polylactic acid, the market demand is huge. It is estimated that the demand for food-grade L-lactic acid is growing at a rate of 5%-8% per year, far exceeding the current supply capacity. In addition, the current annual output of degradable plastics ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/01
CPCC12P7/56C12N1/205C12R2001/01
Inventor 樊亚超张霖师文静
Owner CHINA PETROLEUM & CHEM CORP
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