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Application of chloramine B solution in chicken coccidiosis oocyst culture and preservation

A chicken coccidial oocyst, chloramine technology, applied in the preservation of microorganisms, microorganism-based methods, microorganisms and other directions, can solve problems affecting human and animal health, residues, etc., and achieve the effect of improving sporulation rate and good activity

Inactive Publication Date: 2014-04-09
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
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Problems solved by technology

At present, the oocyst culture solution that can be used in the laboratory includes 0.1% formaldehyde, 1% sulfuric acid and 2.5% potassium dichromate, etc. Usually, 2.5% potassium dichromate solution is used, but potassium dichromate is a heavy metal compound , long-term use will remain in the animal body and the environment, affecting human and animal health

Method used

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  • Application of chloramine B solution in chicken coccidiosis oocyst culture and preservation
  • Application of chloramine B solution in chicken coccidiosis oocyst culture and preservation
  • Application of chloramine B solution in chicken coccidiosis oocyst culture and preservation

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Embodiment

[0010] Next, we use experiments to verify that the chloramine B aqueous solution can be used as a culture medium and a preservation solution for chicken coccidia oocysts.

[0011] 1. Test material

[0012] 1.1 Experimental animals: Lingnan yellow chicks, provided by the Institute of Animal Science, Guangdong Academy of Agricultural Sciences, were raised in a sterilized special animal room; the chicken cages and utensils were strictly sterilized, free to eat and drink pure water; the chicks were observed before the experiment Whether there are clinical symptoms and whether there are coccidia oocysts in the feces for 2 consecutive days shall be checked for backup.

[0013] 1.2 The 102-type broiler feed is customized by Guangdong Xinnandu Feed Co., Ltd., and does not contain any anticoccidial drugs.

[0014] 1.3 Precocious line of Eimeria tenella GD strain, Eimeria gigas GD strain, Eimeria maxima GD strain, precocious line of Eimeria tenacia GD strain precocious line parasitized...

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Abstract

The invention discloses an application of a chloramine B solution in chicken coccidiosis oocyst culture and preservation. According to the invention, study finds out that the sporulation rate of the chicken coccidiosis oocysts can be effectively improved with the chloramine B aqueous solution as a chicken coccidiosis oocyst culture solution. The oocysts of the chicken eimeria tenella, eimeria acevulina, eimeria maxima and eimeria necatrix are not affected in activity if being preserved within 6 months; if being preserved within 8 months, the yield of the oocysts is reduced within 25%; the pathogenicity is not obviously affected. Therefore, the chloramine B aqueous solution can be used as the culture and preservation solution for the chicken coccidiosis oocysts in stead of potassium dichromate.

Description

technical field [0001] The invention relates to the application of chloramine B solution in the cultivation and preservation of chicken coccidia oocysts. Background technique [0002] The cultivation and preservation of the coccidia vaccine oocysts will directly affect the cost of the vaccine and the success or failure of the immunization. At present, the oocyst culture solution that can be used in the laboratory includes 0.1% formaldehyde, 1% sulfuric acid and 2.5% potassium dichromate, etc. Usually, 2.5% potassium dichromate solution is used, but potassium dichromate is a heavy metal compound , long-term use will remain in the animal body and the environment, affecting human and animal health. Thereby urgently need to develop a kind of coccidial vaccine oocyst culture fluid and preservation fluid that are harmless to human body and animals. Contents of the invention [0003] The inventors of the present application have found in research that chicken coccidia oocysts c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/10C12N1/04C12R1/90
Inventor 戚南山孙铭飞廖申权吴彩艳吕敏娜李娟谢明权温列娜
Owner INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
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