Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Solid fermentation method of bacillus coagulans

A technology of Bacillus coagulans and solid fermentation, applied in the field of feed microorganisms, can solve the problems of high cost, low spore formation rate, long fermentation period, etc., and achieve the effects of low cost, high spore formation rate, and large cell concentration

Inactive Publication Date: 2013-09-11
GUANGZHOU GLAM BIOTECH
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a solid fermentation method of bacillus coagulans, to overcome the problems of long fermentation period, low sporulation rate and high cost in the existing solid fermentation method of bacillus coagulans

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The bacillus coagulans is placed in the seed culture solution for recovery and subculture to obtain the seed bacteria solution. Prepare the liquid seed medium according to the following formula: 5g of peptone, 3g of beef extract, 5g of NaCl, 1L of water, and adjust the pH value to 7.0-7.2. The prepared above-mentioned liquid seed medium was sterilized by high-pressure steam at 121°C for 20 minutes. Take the activated preserved bacteria in a 1L culture bottle filled with 200mL liquid seed medium, inoculate 4% (V / V), mix evenly, place in a constant temperature shaker, and cultivate at 30°C and 200r / min for 18h .

Embodiment 2

[0019] The seed bacteria solution prepared in Example 1 was inoculated into the solid fermentation culture medium for cultivation to obtain a solid fermentation product. Prepare the solid fermentation medium according to the following formula: bran: corn = 1:2, add water to make the water content 70%, soak for 2 hours, add 6% glucose, 4% beef extract and 0.1% manganese sulfate to the water, adjust the pH 7.5, autoclaved at 121°C for 20min. Inoculate the seed bacteria solution prepared in Example 1 into the solid fermentation medium with an inoculation amount of 10% (V / V), and incubate at 30°C for 24 hours.

Embodiment 3

[0021] Put the product fermented in Example 2 into a vacuum drying oven at 60°C for vacuum drying to obtain a Bacillus coagulans fermentation product. After multiple dilutions, count on a plate. The content of viable bacteria in the Bacillus coagulans preparation is 1 ×1010cfu / g~1×1011cfu / g.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a solid fermentation method of bacillus coagulans. The method comprises the following steps of: (1) putting the bacillus coagulans in a seed culture solution for recovery passage so as to obtain a seed bacterium solution; (2) inoculating the prepared seed bacterium solution in a sterile solid culture medium for fermental cultivation so as to obtain zymophyte; and (3) drying the prepared fermentation products at 60 DEG C in vacuum so as to prepare finished fermentation bacillus coagulans product. The solid fermentation method of bacillus coagulans disclosed by the invention has the beneficial effects that the fermentation time is 24 hours and is saved, the fermentation period is shortened, besides, the cell concentration is large, spore formation rate is high, common fermentation matrix is used, so the storage and transportation are convenient; not only are no waste liquid and environmental pollution caused, but also the cost is low.

Description

technical field [0001] The invention relates to the field of feed microorganisms, in particular to a solid fermentation method for bacillus coagulans. Background technique [0002] Bacillus-like microbial additive is a kind of living bacteria preparation made from beneficial bacillus in normal microbial group members, which has the advantages of natural high temperature resistance and acid and alkali resistance. Bacillus coagulans (Bacillus subtilis) is a mesophilic aerobic spore-forming rod-shaped bacterium that exists widely in nature. It is non-toxic to humans and animals, does not pollute the environment, and can form spores. Gram-positive bacteria are domestic It is recognized by outsiders that the probiotics can be directly added to the feed. [0003] Bacillus coagulans, also known as Lactobacillus, can produce lactic acid and form spores, and is a strain with great application potential. [0004] At present, the research and application of Bacillus coagulans produc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/07
Inventor 印遇龙王从峰李本涛夏新界何宏轩佘锐萍李帅伟
Owner GUANGZHOU GLAM BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com