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Method for manufacturing silicious-dust-stained rat model

A rat model, a disposable technology, applied in the medical field to achieve the effect of reducing cost, convenient acquisition, and reducing the difficulty of operation

Inactive Publication Date: 2014-05-14
QINGDAO CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, tracheotomy and tracheal intubation are mainly used to prepare silica-dust-stained rats. Among them, tracheotomy is more commonly used, but it has large damage, Disadvantages such as susceptibility to infection
Endotracheal intubation is a fast, non-invasive, and relatively clean intratracheal drug delivery method, but traditional endotracheal intubation is difficult to operate and has a high mortality rate. The main problems are the depth of anesthesia, selection of appropriate caliber, and soft and hard tubes. And how to expose the glottis and other aspects
However, special rat tracheal intubation tubes, laryngoscopes and other equipment are not popular because of their high price and complicated use.

Method used

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Examples

Experimental program
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Effect test

Embodiment Construction

[0016] (2) Prepare the silica dust suspension according to the ratio of 5g silica dust, 800,000 U penicillin for injection, and 1L saline for injection;

[0017] (3) 6-8 weeks old Wistar rats were anesthetized by intraperitoneal injection of 10% chloral hydrate by 3ml / Kg body weight;

[0018] (4) Fix the rat on the operating table in the supine position, place a small backing plate to raise the part below the neck, so that the rat's head is moderately tilted back, and the head side is upward, together with the operating table and the horizontal plane, inclined at an angle of 45°;

[0019] (5) Use hemostatic forceps or other tools to pull the mouse tongue upwards out of the mouth, and at the same time place a flashlight or other light sources on the rat's neck, and observe that the rat's glottis opens and closes with breathing;

[0020] (6) Insert the endotracheal tube when the glottis is open, about 5 cm long from the incisors. If water vapor appears on the inner tube wall, th...

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Abstract

The invention discloses a method for manufacturing a silicious-dust-stained rat model. A disposable vein connecting tube is selected as a tracheal tube, anesthetization is performed on the abdominal cavity of a rat, the rat is fixed to an operation table in a supine position, the part below the neck is cushioned to be high, so that the head is raised backwards, and the head side faces upwards and is inclined by 45 degrees together with the operation table. The rat tongue is upwards pulled out of the oral cavity, meanwhile, a light source is gently placed at the neck of the rat, when the glottis is opened, the tracheal tube is inserted, silicious dust suspension liquid is injected, and then the air is injected. The tracheal tube is fast pulled out, the rat is erected and rotated so that the injected suspension liquid can be evenly distributed, and model manufacturing is finished. The method for manufacturing the silicious-dust-stained rat model is reliable and feasible, used tools are convenient to obtain, and the mold manufacturing success rate is high.

Description

technical field [0001] The invention relates to a method for preparing a rat model contaminated with silica dust, which belongs to the field of medicine. technical background [0002] Pulmonary fibrosis is a respiratory system disease that is extremely harmful to human health. The etiology is complex. At present, its pathogenesis has not been clearly elucidated, and there is no effective treatment. In the field of occupational diseases, pneumoconiosis is a systemic disease mainly caused by diffuse fibrosis of lung tissue caused by long-term inhalation of productive dust during occupational activities and retention in the lungs. Silicosis is one of the twelve types of pneumoconiosis in my country. , researchers often prepare animal models contaminated with silica dust to analyze the pathological changes and find effective intervention methods. [0003] Currently, tracheotomy and tracheal intubation are the main ways to prepare silica dust-infected rats. Among them, tracheot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00
Inventor 张华刘凤娟宋文文
Owner QINGDAO CENT HOSPITAL
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