The invention belongs to the technical field of medical
animal model construction, and discloses a
coal silicosis mouse model construction method and application. According to the method, a
coal-
silicon mixed dust suspension containing 15%, 25% and 50% of free
silicon dioxide is prepared, and the concentration is 50 mg / ml; the method comprises the following steps: hanging an anesthetized healthy adult (6-8 weeks) C57BL / 6N male mouse on a fixing device, instilling 50 microliters of
coal and
silicon mixed dust suspension once per week by adopting an oropharynx
inhalation method for 4 weeks continuously, and establishing a coal
silicosis model; applying equal-volume normal
saline to the control group; respectively observing for 28 days, 56 days and 112 days, taking
mouse lung tissues for
pathological analysis and
fibrosis index detection, and evaluating the model preparation effect. The method is simple to operate and low in cost, and the established coal
silica dust mouse model is stable, efficient, repeatable and noninvasive.