Construction method of human-derived tumor xenograft model

A construction method and xenotransplantation technology, which is applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of malignant tumors endangering human health, and achieve the effects of optimizing screening schemes, accelerating establishment, and fast tumor formation

Inactive Publication Date: 2020-05-08
南京普恩瑞生物科技有限公司
View PDF11 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Malignant tumors have become a major problem that endangers human health. At present, clinical tumor chemotherapy is mostly based on empirical prescriptions, with great uncertainty

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction method of human-derived tumor xenograft model
  • Construction method of human-derived tumor xenograft model
  • Construction method of human-derived tumor xenograft model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] The method for constructing a human-derived tumor xenograft model includes the following steps: (1) Take fresh human gastrointestinal tumor tissue derived from a clinical patient, store it in a preservation solution, and transport it to a laboratory. The preservation solution includes: 100 μL of Double antibody (penicillin and streptomycin) + 3 mL of fetal bovine serum + 6.9 mL of L-15 medium; (2) Select NCG female mice for anesthesia; after the anesthesia takes effect, put the NCG female mice in the right lateral position After disinfection, cut the back skin to expose the kidney to the peritoneum; (3) cut the human gastrointestinal tumor tissue in the preservation solution to a size of 2mm*2mm*2mm; (4) cut the human gastrointestinal tumor tissue The tumor tissue was implanted under the renal capsule of the mouse, and returned to the kidney of the NCG female mouse after successful inoculation, and the wound was sutured; and (5) the NCG female mouse was warmed and revive...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention discloses a construction method of a human-derived tumor xenograft model. The construction method of the human-derived tumor xenograft model comprises the following steps: (1) taking fresh tumor tissues from clinical patients and storing the fresh tumor tissues in a preservation solution; (2) selecting immunodeficient mice for anesthesia; and placing the mice on right side lying positions and cutting skin on the back to peritoneum to expose the kidneys after disinfection; (3) cutting the tumor tissues in the preservation solution; (4) implanting the cut tumor tissues intokidney capsules of the mice, sending the kidneys back into the mice after successful inoculation, and suturing the wound; and (5) warming and recovering the mice and sending the mice back to cage boxes after the mice are awake. The construction method can promote formation of tumor microvessels. Compared with subcutaneous inoculation, tumor growth time is shorter, tumor formation is faster, tumorspecific growth rate is higher, the obtained tumor tissues are larger, modeling success rate of clinical patient-derived tumor xenograft is improved, the human-derived tumor xenograft model can be used to conduct sensitivity experiments on tumor chemotherapy drugs and in-vivo drug efficacy screening can be done in the shortest time.

Description

technical field [0001] The invention relates to the technical field of tumor transplantation, in particular to a method for constructing a human-derived tumor xenograft model. Background technique [0002] Malignant tumors have become a major problem that endangers human health. At present, clinical tumor chemotherapy is mostly based on empirical prescriptions, with great uncertainty. At present, the individualization of tumor treatment and the certainty of clinical efficacy eliminate the damage of ineffective chemotherapy drugs to patients, which is an urgent requirement for clinical chemotherapy. The drug sensitivity test of tumor chemotherapy is the best way to solve this problem. Human-derived tumor tissue xenografts simulate the tumor growth environment and in vivo drug environment, and have broad development prospects. [0003] Human-derived tumor tissue xenotransplantation (PDTX) technology refers to the transplantation of fresh tumor tissue from tumor patients into...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 朱燕萍王志强郝艳鹏陈馨叶琳璐
Owner 南京普恩瑞生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap