Construction method of human-derived tumor xenograft model

Abstract

The present invention discloses a construction method of a human-derived tumor xenograft model. The construction method of the human-derived tumor xenograft model comprises the following steps: (1) taking fresh tumor tissues from clinical patients and storing the fresh tumor tissues in a preservation solution; (2) selecting immunodeficient mice for anesthesia; and placing the mice on right side lying positions and cutting skin on the back to peritoneum to expose the kidneys after disinfection; (3) cutting the tumor tissues in the preservation solution; (4) implanting the cut tumor tissues intokidney capsules of the mice, sending the kidneys back into the mice after successful inoculation, and suturing the wound; and (5) warming and recovering the mice and sending the mice back to cage boxes after the mice are awake. The construction method can promote formation of tumor microvessels. Compared with subcutaneous inoculation, tumor growth time is shorter, tumor formation is faster, tumorspecific growth rate is higher, the obtained tumor tissues are larger, modeling success rate of clinical patient-derived tumor xenograft is improved, the human-derived tumor xenograft model can be used to conduct sensitivity experiments on tumor chemotherapy drugs and in-vivo drug efficacy screening can be done in the shortest time.

Description

technical field [0001] The invention relates to the technical field of tumor transplantation, in particular to a method for constructing a human-derived tumor xenograft model. Background technique [0002] Malignant tumors have become a major problem that endangers human health. At present, clinical tumor chemotherapy is mostly based on empirical prescriptions, with great uncertainty. At present, the individualization of tumor treatment and the certainty of clinical efficacy eliminate the damage of ineffective chemotherapy drugs to patients, which is an urgent requirement for clinical chemotherapy. The drug sensitivity test of tumor chemotherapy is the best way to solve this problem. Human-derived tumor tissue xenografts simulate the tumor growth environment and in vivo drug environment, and have broad development prospects. [0003] Human-derived tumor tissue xenotransplantation (PDTX) technology refers to the transplantation of fresh tumor tissue from tumor patients into...

AI Technical Summary

Problems solved by technology

[0002] Malignant tumors have become a major problem that endangers human health. At present, clinical tumor chemotherapy is mostly based on empirical prescriptions, with great uncertainty

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