Method for screening a fish liver protecting drug by using carp liver injury model induced by CCl4

Abstract

The present invention discloses a method for screening a fish liver protecting drug by using a carp liver injury model induced by CCl4. According to the method, a drug requiring test is added to a carp feedstuff; after oral administration for 1-2 months, a preferred CCl4 vegetable oil solution with concentration of 25-30% is injected to the carps by peritoneal injection according to a certain dose, wherein 10 g of the carp is injected with 0.05 mL of the solution; after injection for 48-72 hours, changes of serum GPT activity, serum GOT activity and serum LDH activity are adopted to evaluate liver protecting activities of different tested drugs. The carp generating hepatobiliary syndrome is adopted as the biological model so as to provide strong target property, wherein the carp is the main hazardous object of the hepatobiliary syndrome; the drug is taken by the oral administration so as to provide convenience and practicality; the blood liver function index changes are adopted to evaluate the liver protecting activity of the tested drug without tested fish sacrificing so as to provide strong operability. The experimental results show that: the method for screening the fish liver protecting drug by using the carp liver injury model induced by the CCl4 has characteristics of accurate results, strong repeatability, low cost, wide application range, batch screening and high efficiency.

Description

technical field [0001] The present invention relates to a kind of screening method of aquatic medicine, be specifically related to a method for using carbon tetrachloride (CCl 4 )-induced carp liver injury model to screen fish hepatoprotective drugs. Background technique [0002] In recent years, due to environmental pollution, the use of high-protein, high-fat or moldy feed, and the abuse of antibiotics and pesticides, fish hepatobiliary syndrome has frequently occurred in many parts of the country, causing carp, crucian carp, grass carp, etc. The large-scale death of multiple fishes such as bream, bream and herring, the mortality rate is as high as 60-90%, which causes great economic losses to the aquaculture industry. [0003] The etiology of fish hepatobiliary syndrome is diverse, but its common feature is that it causes damage to the liver tissue of fish, which in turn causes metabolic disorders and causes bacterial and viral infections, resulting in the death of fish....

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Problems solved by technology

[0005] Chinese patent 201010170675.2 discloses a method for screening anti-liver injury drugs using the model organism zebrafish. Although the zebrafish is an aquatic animal as a model organism in this patent, the anti-liver injury drugs screened have no effect on the prevention of liver disease in fish in aquaculture. Not applicable

First of all, the invention uses CCl 4 Soak the zebrafish to induce liver damage, and then add the test drug into the water to soak the zebrafish with liver damage for 3 days, so this invention is actually to screen drugs for the treatment of liver damage rather than preventive drugs, and the prevention and treatment of aquatic animal diseases is mainly prevention Secondly, the mode of administration of this invention is that medicine is soaked for 3 days, if also administer medicine by soaking mode and use in the whole pond in production, will certainly need very large medicine dose, if the fish in the pond is concentrated medicine soaking for as long as 3 days, although the dosage can be reduced, but the operation is extremely difficult, and it is very easy to cause the death of fish due to hypoxia, which is not practical; in addition, because the zebrafish is small, the length is only 3-4 cm, and the weight is less than 1 gram, it is difficult to draw blood. It is very difficult to evaluate the anti-liver injury activity of the test drug by measuring the changes of alanine aminotransferase (GPT) and aspartate aminotransferase (GOT) in the serum, only the GPT and GOT in the liver homogenate can be detected, so the zebra The fish is killed, and its very small liver is taken out to make a liver homogenate, which is difficult to operate

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