91 results about "CCL4" patented technology

This disclosure relates to methods of selecting a subset of subjects having a hematological disorder and treating the selected group with a PI3K-delta inhibitor. In particular, the methods disclose evaluating levels of characteristic chemokine biomarkers, such as CCL2, CCL3, CCL4, CCL5, CXCL13, CCL17, CCL22, or TNF-alpha to select subjects that would have a greater chance of benefiting from treatment with a PI3K-delta inhibitor. The PI3K-delta inhibitors disclosed in this application are a type of quinazolinone-purinyl family of compounds.

The invention relates to an engineered immune cell, and the engineered immune cell expresses (i) a chimeric receptor, and (ii) exogenous CCL3, CCL4 and/or CCL5. The invention further provides application of the engineered immune cell to treatment of cancers, infections or autoimmune diseases. Compared with a traditional engineered immune cell, the engineered immune cell provided by the invention has the remarkably improved tumor killing activity.

The invention discloses a Chinese date leaf extractive and an application thereof in preparation of a medicament or health food for preventing and treating liver injury. The way that macroporous resin is coupled with polyamide resin and then a fine Chinese date leaf extractive is prepared through a preparative liquid phase is firstly adopted; compared with the traditional separation and purification method, the way has the advantages that the separation efficiency is higher, the active component obtained through separation is higher in content, strong in biological activity and high in safety. Experiment researches show that the Chinese date leaf extractive can be used for remarkably reducing the activities of ALT (Alanine Transaminase) and AST (Aspartate Transaminase) in serum of a model mouse with liver injury caused by CCl4, D-GalN and ethanol and the content of MDA (Methyl Di Aldehyde) in liver tissues and enhancing the activity of SOD (Superoxide Dismutase) of the liver and has favorable preventing and treating effects. Therefore, Chinese date leaves and the Chinese date leaf extractive are expected to be developed into a new-generation safe and effective medicament or health food for preventing and treating liver injury, and meanwhile, an approach is also provided for turning the waste Chinese date leaf resource into the things of value and comprehensively utilizing a date tree resource.

The invention relates to a screening method for a medicine for targeting NS5ATP9 (HCV NS5A-transactivated protein 9) prevention or treatment of tissue fibrosis and tissue sclerosis, and the medicine obtained by screening using the method. NS5ATP9 significantly regulates the expression of extracellular matrix collagen and non-collagen glycoprotein and affects the occurrence and development of tissue fibrosis by regulating a TGFbeta1/Smad3 signaling pathway; and the NS5ATP9 promotes the apoptosis of hepatic stellate cells, inhibits the proliferation of the hepatic stellate cells and regulates extracellular matrix deposition by regulating the phosphorylation level and the intracellular translocation condition of a Smad3 protein. TDF/TAF can effectively regulate the expression of NS5ATP9 gene,and is verified in CCl4-induced mouse liver fibrosis model, and the TDF/TAF achieves significant effects in the prevention or treatment of CCl4-induced liver fibrosis in mice.

The invention discloses a method for synthesizing 2,4-dichloro-5-fluorobenzoyl. The method comprises the following steps: reacting 2,4-dichlor fluorbenzene and CCl4 serving as initial raw materials under actions of solid acid catalyst S2O82-/Sm2O3-ZrO2-Al2O3 and AlCl3 to generate 2,4-dichloro-5-fluoro-(trichloromethyl) benzene, and catalyzing and hydrolyzing by FeCl3. The method has the advantages that: the solid acid catalyst S2O82-/Sm2O3-ZrO2-Al2O3 can be conveniently recycled, the yield is high, so that the proportion of biopolymer byproducts can be reduced; use of high-pollution high-risk raw materials can be avoided, the operation safety is high, and pollutant emission is little; and the method can be used for breaking through the technical bottleneck of the reaction of aryl halide and CCl4, and massively consuming CCl4 which destroys the ozone layer by introducing acyl chloride functional group on an aromatic ring by adopting the CCl4 as a raw material.

The invention discloses a method for determining the content of cadmium in soil by ICP-AES (Inductively Coupled Plasma-Atomic Emission Spectrometry). The method adopts an extraction-reextraction system to deal with a soil sample digested by mixed acid and solves the problem of shifting of the cadmium spectral absorption peak, caused by concentration of cadmium in low-cadmium soil digestion solution and soil matrix. The method comprises the steps of enabling cadmium ions in the digestion solution and sodium diethyldithiocarbamatre to form a chelate in mediums with certain pH value, then performing extraction separation by CCl4 organic phase, adding with nitric acid-hydrogen peroxide mixed solution to oxidize and destroy the chelate, performing reextraction to cadmium in water phase and determining the content of cadmium; experimental results show that the method is simple and feasible, has high precision and good accuracy, can determine low cadmium content of the soil, can make up the blank that the ICP-AES cannot accurately determine the cadmium content in the soil and provides new reference for detection analysis of the cadmium content of the soil.

The invention provides a preparation method of fibrauretine, mainly comprising the following steps of: (1) using 3-halo-7,8-dimethoxy isoquinoline of a formula I and 2-(2-hydroxyethyl)-4,5-dimethoxy boric acid of a formula II as raw materials and synthesizing a first intermediate under the actions of a palladium catalyst, alkali and protective gas; (2) further synthesizing the first intermediate into a second intermediate under the actions of Ph3P, CCl4 and acetonitrile; (3) synthesizing the second intermediate into the fibrauretine under the action of an acid-binding agent, wherein in the 3-halo-7,8-dimethoxy isoquinoline of the formula I, X stands for any one of Cl, Br and I. In the invention, the preparation method has a shorter synthetic process line, the used starting materials are synthetic materials and are not limited by natural resources, and the used ancillary materials have lower environment pollution and no amounts of water to discharge; and the invention has the advantages of low synthetic cost and simple operation, and the obtained product has high purity.

The invention discloses a preparation method of phosphorus-containing triazole compound and application of the phosphorus-containing triazole compound in flame-retardant polylactic acid. The preparation method includes: putting DOPO (9,10-dihydro-9-oxa-10-phosphaphenanthrene-10- oxide) and CH2C12 into a three-opening flask, adding a certain amount of triethylamine, adding 3-amino-1,2,4-triazole after materials in the flask are dissolved, adding CCl4 dropwise, extracting and filtering after reaction is cover, taking filter cakes, washing and drying to obtain the phosphorus-containing triazole compound. The preparation method of the phosphorus-containing triazole compound is simple in processing steps and postprocessing processes, the obtained phosphorus-containing triazole compound is highin purity. The phosphorus-containing triazole compound can be applied to the flame-retardant polylactic acid and is highly compatible with the polylactic acid, a flame-retardant polylactic acid material prepared from the phosphorus-containing triazole compound is excellent in flame retarding performance and has better flame retarding performance as compared with pure polylactic acid, which means that D-ATA has a great flame-retardant effect on the polylactic acid under the condition of low addition amount.

A preparation method of chronic hepatic injury complicated with stress depression rat model comprises selecting male Wistar rat (body weight of 180-220 g), acclimating for one week, performing open field test ethology screening, and selecting rat having close integration; normally drinking and eating for the first week to the fourth week, and performing hypodermic injection of 40% CCL4 1ml/kg (twice per week); normally drinking and eating from the fifth to the twelfth week, performing hypodermic injection of 40% CCL4 1ml/kg (twice per week), and performing gastric perfusion of normal saline (one time per day); normally drinking and eating in the thirteenth week, and performing gastric perfusion of normal saline (one time per day); performing open field test in the fourteenth to sixteenth week (one rat per cage), normally drinking and eating, performing gastric perfusion of normal saline (one time per day), and offering chronic stress stimulation; and weighing in 112th day, and performing sweet water consumption and open field test. The preparation method provides reference for modeling research of chronic hepatic injury complicated with stress depression, provides effective experiment base for clinical diagnosis of hepatopathy complicated with depression, and provides experimental verification means for the research of drug for treating the hepatopathy complicated with depression.

The invention provides a method for establishment of a macaca mulatta liver fibrosis model by intraperitoneal injection. The method includes the steps of: selecting 5-9kg male macaca mulattas of 2-3 years old as the model animals, performing feeding with high-fat feed during modeling, supplying an alcohol water solution with a volume concentration of 9%-11% for drinking, and injecting CCl4 injection to each macaca mulatta during modeling twice a week by intraperitoneal injection continuously for 20-30 weeks, and controlling the injecting amount at 0.8-1.5mL/kg each time, thus achieving successful modeling. The animal model establishment method provided by the invention is simple, safe and reliable, has the characteristics of short modeling time, high success rate, good repeatability, goodanimal tolerance and low death rate, and can be used for studying the pathogenesis, pathogenic factors and treatment means of chronic liver diseases.

The application discloses a liver fibrosis model as well as a construction method and an application thereof. The liver fibrosis model is induced by CCL4 through specific steps as follows: 8-week-oldmale mice are intraperitoneally injected with CCL4 in an amount of 3 ml/kg body weight, CCL4 is dissolved in olive oil in a ratio of 1:4, and control mice are only injected with olive oil twice a weekand injected continuously for 6 weeks; liver samples are taken 40 days after the first injection. Compared with mouse livers treated with oil, induction of target sites in the mouse livers treated with CCL4 is about 2-3 times, and the target expression reaches peak on the 5th day.

The present invention relates to the field of biomedicine. The present invention provides application of a farnesoid X receptor (FXR) expression interference reagent in preparation of anti-hepatic fibrosis drugs. Specifically, the present invention relates to application of FXR for treatment of hepatic fibrosis, by tail intravenous injection of the farnesoid X receptor (FXR) expression interference reagent (namely, an adenovirus carrying FXR plasmid) to mice, the expression level of the FXR in hepatocytes is significantly increased, CCl4-induced hepatic fibrosis can be significantly countered,hepatocyte morphology and structural integrity can be maintained, and serum transaminase level can be reduced. A new approach for clinical treatment of liver diseases especially hepatic fibrosis is provided.

The invention belongs to the field of molecular biology, relating to the applications of Grp75 in the preparation of drugs for the treatment of oxidative damages of liver cells and liver fibrosis by using gene transfection techniques. According to the invention, based on that Grp75 is an important chaperone in cytoplasm and mitochondria, wherein the 1-23 loci of the N-terminal thereof is a mitochondrial transmembrane signal, can help a protein to be correctly folded, assist the transport of the protein, and has the characteristics such as removal of ROS and antiapoptosis, experiments of cytoprotective effects of Grp75 on H2O2-induced oxidative stress in cells, and protective effects on CCL4-induced hepatic fibrosis as a pathological basis in animal models are carried out. When an injury occurred in the liver cells, the GRP75 gene is transfected in the cells, and the expressions of Grp75 protein are upregulated respectively at a cells level and a whole level; and results show that the effect of the treatment of liver fibrosis can be achieved through the effects of anti-oxidative damage and inhibition of hepatocyte apoptosis of the Grp75 protein. The Grp75 gene of the present invention can be used for preparing drugs for the treatment of liver fibrosis.

The invention discloses a synthetic peptide vaccine of B cell epitope based on TGF(transforming growth factor)-beta1 and application thereof, inhibits auxo-action of the cell factor TGF-B1-BB during the development process of liver fibrosis so as to inhibit the development of liver fibrosis to a some extent by constructing two epitope peptides of AY25 and LS30, coupling carrier proteins to synthesize an TGF-beta1 peptide vaccine, immunizing an ICR mice by intraperitoneal injection to induce the same to generate an anti-TGF-beta1 antibody, and neutralizing the cell factor TGF-B1-BB over expressed during CCL4 induced hepatic fibrosis process.

The invention discloses a preparation method for metallic uranium, relates to the field of methods for preparing uranium, and specifically relates to the field of methods for preparing uranium by applying a molten salt electrochemistry technology. The specific technical scheme is as follows: U3O8 is heated to remove water, and CCl4 gas and high-purity nitrogen gas are mixed and charged into a quartz pipe for 2-6h at 400-460 DEG C to obtain UCl4; a product A is mixed with the mixed molten salt of LiCl and KCl, electrification is carried out for 1h to 20h at a current of 0.1A to 1A and at 673-873K, and a material metallic uranium crude product or an electrode made from U-Ga alloy is taken out; and a material B is taken as an anode, a molybdenum sheet is taken as a cathode, and electrolysis is carried out for 1-40h at 0.1A-1A, and high-purity metallic uranium can be separated from B. The embodiments indicate that the preparation method for metallic uranium is capable of greatly increasingthe separation speed for metallic uranium and greatly improving the purity of metallic uranium, and significant to nuclear power construction in China to a certain extent.

The invention discloses a triterpenoid AntcinK and liver-protecting activity and application thereof. The triterpenoid AntcinK is extracted from wild, basswood-cultured and/or plate-cultured antrodia camphorate sporocarps, multiple activity screenings prove that the triterpenoid has the outstanding activity of protecting acute hepatitis liver damage caused by CCl4 and the activity of protecting abnormal lipid accumulation induced by alcohol. The compound has the remarkable preventing and treating effect on acute hepatitis, liver damage and alcoholic fatty liver and can be widely applied to liver protecting drugs and health-care products.

The invention discloses a preparation method of 2,3,3,3-tetrafluoropropene. The preparation method comprises the steps: performing telomerization on chloroethylene and CCl4 so as to produce 1,1,1,3,3-pentachloropropane, then performing dehydrochlorination so as to generate 1,1,3,3-tetrachloro-1-propylene, performing exchange of fluorine and chlorine and dehydrochlorination so as to prepare 3,3,3-trifluoro-1-chloropropene, then performing an isomerization reaction so as to obtain 3,3,3-trifluoro-2-chloropropene, performing a liquid phase fluorination reaction so as to synthesize 1,1,1,2-tetrafluoro-2-chloropropane, and finally performing dehydrochlorination so as to obtain 2,3,3,3-tetrafluoropropene. The raw materials have low price, and are easy to obtain, so that the raw material cost inthe production process of 2,3,3,3-tetrafluoropropene is reduced; an industrial chain is formed in the reaction process, and highly-economic values are provided through purification of the intermediateproducts; the reaction conditions are mild, the equipment has simple operation, the raw material conversion rate and product selectivity are high at the reaction stages, and by-products are few, so that a large industrial application prospect is achieved.

The invention provides a preparation method of a carbonized Pd (palladium)-carrying hollow anion exchange resin microsphere H2O2 (hydrogen peroxide) electroreduction catalyst. The preparation method is characterized by comprising the following steps: mixing styrene, divinylbenzene and deionized water, introducing N2 (nitrogen), adding CCl4 (tetrachloromethane), then adding K2S2O8 (potassium persulfate) and NaCl (sodium chloride), enabling the mixture to react for 2h to 3h at the temperature of 70DEG C, adding methylacryloyl chloroethyl trimethyl ammonium chloride to carry out grafting polymerization reaction for 12h to 15h, washing the reaction product by utilizing ethanol in a low-temperature trough, centrifugally separating the reaction liquid, and carrying out vacuum drying on the reaction product for 4h to 5h at the temperature of 50 DEG C; immersing the anion exchange resin in H2PdCl4 solution for 5h to 6h, taking out the anion exchange resin, and drying the anion exchange resin for 4h to 5h at the temperature of 50 DEG C; calcining the Pd-carrying anion exchange resin microsphere for 5h to 6h in a nitrogen atmosphere at the temperature of 650 to 750DEG C to obtain the carbonized Pd-carrying anion exchange resin microsphere H2O2 electric reduction catalyst. By transplanting Pd catalyst in the hollow carbon microspheres, the electrochemical reduction activity of the catalyst on the H2O2 can be improved, the discharging performance of a H2O2 cathode can be improved, and the escaping of oxygen can be inhibited.

The invention provides application of rutabaga in preparation of a drug for protecting liver. Found by action mechanism research, a Tibetan medicine rutabaga polysaccharide can improve the antioxidantcapacity of the liver of a CCl4-induced acute liver injury mouse model, and significantly reduce the oxidative damage of the liver, can inhibit the expression of caspase3 protein in the liver of a CCl4-induced acute liver injury mouse, reduces the apoptosis of hepatocytes, and has a protective effect on CCl4-induced acute liver injury of the mouse. In terms of liver protection, rutabaga polysaccharide has both the prevention and treatment effects, and has a good curative effect, is highly safe and is widely applied.

The invention discloses an application of apple polyphenols for preparing liver-protecting medicines, health-care foods or foods. The experimental result proves that the apple polyphenols have liver protecting effect, have obvious protecting effect on liver injury models caused by various chemical substances such as CCl4, ethanol, D-GalN, cis-platinum and the like, and have colagogue action; and the AP also has protecting effect on immunological liver injury.

The invention relates to the use of Fraxinellone as medicament for treating hepatic diseases, wherein the Fraxinellone is separated from the velamen of Dictamnus dasycorpus Turcz, the Fraxinellone can substantially improve acute hepatic injury in mouse induced with CCl4, D-galactosamine and lipopolysaccharide, in addition, the Fraxinellone can substantially inhibit the release of glutamate-pyruvate transaminase in CCl4 induced mouse primary culture hepatocytes injuries, thus can be used as a novel medicament for treating hepatic diseases.

The invention discloses a novel triterpenoid saponin compound with a liver protection effect. By systematically and deeply studying chemical components of liquorice, a new triterpenoid saponin compound is separated from licorice through wave spectrum and mass spectrometric data analysis. The pharmacological experimental study represents that the triterpenoid saponin compound disclosed by the invention is capable of obviously reducing CCl4 induced liver injury model mice, ethanol induced liver injury model mice, D-galactosamine induced liver injury model mice serum ALT (alanine aminotransferase), AST (aspartate amino transferase) activity and liver tissue MDA (methylene dioxyamphetamine) content and enhancing liver SOD (superoxide dismutase) activity, and the compound has an obvious protection effect on the liver injury; the toxicity test study represents that the triterpenoid saponin compound with the liver protection effect, which is disclosed by the invention, has no obvious toxicity, and can be hopefully developed as a novel medicine or healthcare product with liver protection effect.

The invention belongs to the technical field of detection, and in particular relates to a method for detecting silver ions by adopting a thiourea complexation method. The method comprises the following steps: (1) measuring a silver-containing water sample, and putting into a beaker; (2) weighing a thiourea sample for later use; (3) heating the silver-containing water sample obtained in the step (1), adding the thiourea sample obtained in the step (2) into the water sample and then continuously stirring at constant temperature until thiourea is completely dissolved; (4) continuously stirring the solution obtained in the step (3) for 24h or more so as to enable Ag+ and thiourea to completely react and generate Ag+-thiourea complex; (5) pouring the solution obtained in the step (4) into a conical flask, adding CCl4 into the conical flask and shaking up; (6) enabling the solution obtained in the step (5) to stand for 2h and then extracting, taking out extract liquor at the lower layer, and detecting the content of the silver ions by using an inductively coupled plasma-atomic emission spectrometry (ICP-AES). The method provided by the invention can eliminate the interference of other metal ions so as to improve the test accuracy; the method is simple and easy to operate, and improves the sensitivity of a detection result.