Preparation method of 2-type diabetic nephropathy model

Abstract

The invention relates to a preparation method of a 2-type diabetic nephropathy model. The method comprises the following steps of: selecting a male SHR (spontaneously hypertensive rat) rat with an age of 6 weeks, wherein the blood pressure of the male SHR rat at birth is normal, and at the time of 4 weeks, the blood pressure begins to rise; after adaptively feeding the SHR rat selected in the step (1) for one week, feeding a high sugar and high fat feed for 2 weeks; detecting the weight, the blood fat, the blood sugar and the insulin concentration and calculating an insulin resistance index evaluated by a steady-state model, wherein the insulin resistance index is not smaller than 3.8, and the high fat and high sugar feed comprises the following components in percentage by weight: 10.0 percent of lard, 20.0 percent of cane sugar, 2.5 percent of cholesterol, 2.0 percent of cholate and 66.5 percent of conventional feed; after fasting the insulin resistant SHR rat for 12 hours, carrying out a small-dose one-time STZ (streptozocin) (35mg/kg) intraperitoneal injection on the SHR rat, wherein the small dose is 35mg/kg; after 72 hours, measuring the random blood sugar; and judging the 2-type diabetic nephropathy model to be successfully prepared if the plasma glucose is not smaller than 16.7mmol/L.

Description

technical field [0001] The invention relates to a simulated object for researching diabetic nephropathy, in particular to a method for preparing a type 2 diabetic nephropathy model. Background technique [0002] Diabetes has increasingly become a global public health problem, usually divided into two types, of which type 2 diabetes accounts for more than 90% of the total diabetes. Diabetic nephropathy is one of the common chronic complications of diabetes, and its pathogenesis is still not very clear. More and more studies have confirmed that podocyte injury plays a very important role in the pathogenesis of diabetic nephropathy. Podocytes are highly differentiated end-stage cells. The existing mouse immortalized podocytes provide an important guarantee for the study of podocyte injury in vitro. In vivo studies, we will establish a model that mimics the pathogenesis of human type 2 diabetic nephropathy as much as possible. Animal models will undoubtedly help us understand ...

AI Technical Summary

Problems solved by technology

At present, there are few research models on the early kidney damage of type 2 diabetic nephropathy. Diabetic model preparation methods include spontaneous or genetic animal models, chemical drugs and drug induction methods, high-sugar and high-fat diet induction methods, surgical resection of the pancreas, and transgenic animals. However, the models established by these methods cannot fully reflect the pathological and pathophysiological changes of type 2 diabetes.

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