Method for building diabetic nephropathy microinflammation mice model

A technology for diabetic nephropathy and mouse model, applied in the field of life sciences, can solve the problems of micro-inflammation research models of diabetic nephropathy that have not yet been discovered, and achieve the effect of simple operation and high modeling success rate.

Inactive Publication Date: 2013-08-14
SOUTHEAST UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] No research model of micro-inflammation related to diabetic nephropathy has been found so far

Method used

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  • Method for building diabetic nephropathy microinflammation mice model
  • Method for building diabetic nephropathy microinflammation mice model
  • Method for building diabetic nephropathy microinflammation mice model

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0034] model group

[0035] Step 1. Select 20 8-week-old male db / db mice with a body weight of 30-40 g. The temperature of the feeding environment was 20±2°C. The rats were reared in separate cages, fed with common pellet feed, free to drink water, alternately illuminated for 12 hours, and adaptively fed for one week. The animal experiments complied with the requirements of the National "Regulations on the Administration of Experimental Animals" and the "Implementation Measures for the Administration of Experimental Animals in Jiangsu Province".

[0036] Step 2. Each mouse selected in step 1 was subcutaneously injected with 0.5 ml of casein solution with a mass concentration of 10% on the back every other day for 8 weeks to stimulate the generation of chronic persistent micro-inflammatory response.

[0037] Step 3. Perform clinical biochemical indicators and histopathological analysis on the mice obtained in step 2 to obtain a micro-inflammation mouse model of diabetic nephro...

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Abstract

The invention discloses a method for building a diabetic nephropathy microinflammation mice model. The method comprises the steps of choosing db / db mice, inducing a microinflammation state through casein subcutaneous, and carrying out histopathological analysis on clinical biochemical indexes and tissues. The method has the characteristics of being simple to operate, economic and practical, high in modeling success rate and the like; the disease features of human diabetic nephropathy can be simulated; and the method has the characteristics of microinflammation, glomerular mesangial matrix proliferation, glomerular sclerosis and the like. Building of the method benefits for looking into the damage mechanism of the diabetic nephropathy; and occurrence and development of the diabetic nephropathy are well prevented.

Description

technical field [0001] The invention belongs to the technical field of life sciences, and in particular relates to a method for establishing a micro-inflammation mouse model of diabetic nephropathy. Background technique [0002] Diabetic nephropathy (DN) is a destructive kidney disease and the main cause of end stage renal disease (ESRD). The prevalence has gradually increased in the past 10 years and has exceeded 50%. Diabetic nephropathy is characterized by glomerular hypertrophy, thickened basement membrane, impaired glomerular filtration barrier, glomerular matrix hyperplasia, advanced glomerulosclerosis, tubulointerstitial fibrosis, progressive proteinuria and renal failure. Functional failure. However, the specific mechanism of diabetic nephropathy is not completely clear. Recent studies have shown that many factors are involved in the mechanism of DN, including proteinuria, heredity, race, hypoxia, ischemia, and inflammation. Among them, inflammation is considered t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17
Inventor 马坤岭张洋
Owner SOUTHEAST UNIV
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