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Breast cancer bone metastasis laboratory mouse disease model building method

A disease model and construction method technology, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of high operation difficulty and low success rate of modeling, so as to improve the success rate of modeling and tumor survival rate effect

Inactive Publication Date: 2021-05-07
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two methods often have a low success rate of modeling due to the mouse's own immune system, and the operation is difficult

Method used

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  • Breast cancer bone metastasis laboratory mouse disease model building method
  • Breast cancer bone metastasis laboratory mouse disease model building method
  • Breast cancer bone metastasis laboratory mouse disease model building method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The method for constructing the experimental mouse disease model of breast cancer bone metastasis in the present embodiment comprises the following steps:

[0040] S1: choose 20g female mice, give 1% ketamine intraperitoneal injection, the anesthesia dose is 4ml / kg;

[0041] S2: After the anesthesia is completed, move to the sterile operating table, fix it in the lateral position, remove about 1cm*1cm of hair on the outer thigh of the mouse along the longitudinal axis of the femur, and the removed area coincides with the femur, and disinfect it with 1% iodine for 3 times. and wipe clean with sterile gauze;

[0042] S3: Cut the skin 0.8 cm along the longitudinal axis of the femur, bluntly separate the subcutaneous fascia, and bluntly separate the muscle space between the lateral rectus femoris and semitendinosus with a small hemostat to expose the distal end of the femur (pay attention to protection during separation) medial femoral artery located in the femur);

[004...

Embodiment 2

[0048] The method for constructing the experimental mouse disease model of breast cancer bone metastasis in the present embodiment comprises the following steps:

[0049] S1: choose 20g female mice, give 1% ketamine intraperitoneal injection, the anesthesia dose is 4ml / kg;

[0050] S2: After the anesthesia is completed, move to the sterile operating table, fix it in the lateral position, remove about 1cm*1cm of hair on the outer thigh of the mouse along the longitudinal axis of the femur, and the removed area coincides with the femur, and disinfect it with 1% iodine for 3 times. and wipe clean with sterile gauze;

[0051] S3: Cut the skin 0.8 cm along the longitudinal axis of the femur, bluntly separate the subcutaneous fascia, and bluntly separate the muscle space between the lateral rectus femoris and semitendinosus with a small hemostat to expose the distal end of the femur (pay attention to protection during separation) medial femoral artery located in the femur);

[005...

Embodiment 3

[0064] The method for constructing the experimental mouse disease model of breast cancer bone metastasis in the present embodiment comprises the following steps:

[0065] S1: choose 25g female mice, give 1% ketamine intraperitoneal injection, the anesthesia dose is 4ml / kg;

[0066] S2: After the anesthesia is complete, move to the sterile operating table, fix in the lateral position, and remove about 1.5cm*1.5cm of hair on the outer thigh of the mouse along the longitudinal axis of the femur. and wipe clean with sterile gauze;

[0067] S3: Cut the skin 0.8 cm along the longitudinal axis of the femur, bluntly separate the subcutaneous fascia, and bluntly separate the muscle space between the lateral rectus femoris and semitendinosus with a small hemostat to expose the distal end of the femur (pay attention to protection during separation) medial femoral artery located in the femur);

[0068] S4: Use tooth forceps to clamp the proximal 1 / 3 of the femur, ophthalmic scissors to ...

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Abstract

The invention provides a breast cancer bone metastasis laboratory mouse disease model building method. The method comprises the following steps: selecting a mouse, anesthetizing the mouse, removing hair on the outer side of the thigh of the mouse, disinfecting the removed area, cutting open the skin of the mouse along the longitudinal axis of the femur, bluntly separating subcutaneous fascia, exposing the distal end of the femur, breaking the continuity of the femur of the mouse to form incomplete fracture, injecting tumor cells into the fracture part of the mouse, quickly closing the intermuscular space, washing the skin operation area of the mouse, injecting energy liquid into the abdominal cavity, and placing the mouse in a resuscitation box. According to the breast cancer bone metastasis laboratory mouse disease model building method provided by the invention, the tumor formation time is shortened by simulating bone tumor invasion in a bone dissolving state in a discontinuous fracture environment, and a bone marrow immune environment can be destroyed, so that immune escape of the tumor cells is realized, the attack of an immune system is avoided, tumors are formed, the survival rate of the tumors is increased, and the modeling success rate is increased.

Description

technical field [0001] The invention relates to the technical field of mouse model building, in particular to a method for building a breast cancer bone metastasis experimental mouse disease model. Background technique [0002] Breast cancer can spread to almost any area of ​​the body. The most common sites are lymph nodes, lung, bone, and liver. At present, breast cancer research is mainly based on breast cancer models established by rodents. In the field of clinical breast cancer bone metastasis, experimental animals are often used to make breast cancer bone metastasis models. The existing disease model construction is roughly divided into three methods. : The first is transplantation, that is, implanting tumor cells in the subcutaneous or muscle tissue. This method is not convincing because it lacks the same physiological environment as the clinical lesion; the second is injection in the mammary gland area of ​​experimental animals. Wait for its spontaneous bone metasta...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61D7/00
CPCA61D1/00A61D7/00
Inventor 闫晨晨刘国辉米博斌熊元李卉
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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