Breast cancer bone metastasis laboratory mouse disease model building method

A disease model and construction method technology, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of high operation difficulty and low success rate of modeling, so as to improve the success rate of modeling and tumor survival rate effect

Inactive Publication Date: 2021-05-07
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These two methods often have a low success rate of modeling due

Method used

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  • Breast cancer bone metastasis laboratory mouse disease model building method
  • Breast cancer bone metastasis laboratory mouse disease model building method
  • Breast cancer bone metastasis laboratory mouse disease model building method

Examples

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Example Embodiment

[0038]Example 1

[0039]The construction method of breast cancer bone metastasis in this embodiment includes the following steps:

[0040]S1: Select 20 g of female mice, give 1% ketamine abdominal injection, anesthesia is 4 ml / kg;

[0041]S2: After the anesthesia is improved to the sterile operator, the side rejection is fixed, and the hair is removed from the outer side of the femur to the outside of the mouse thigh, the elimination area is coincident with the femur, and the use of 1% vital iodine is 3 times. And wipe it with a sterile gauze;

[0042]S3: 0.8cm in the femur vertical axis, bluntly separated the subcutaneous film, the muscle gap between the straight muscle between the outer femoral muscle and the semicoprolaus is depolically separated, exposed the distal end of the femur (pay attention to protection when separating Inner strand artery located in the femur);

[0043]S4: At the nearly 1 / 3 of the femur using the teeth, the ophthalmic shear is held in the distal end of the femur and t...

Example Embodiment

[0047]Example 2

[0048]The construction method of breast cancer bone metastasis in this embodiment includes the following steps:

[0049]S1: Select 20 g of female mice, give 1% ketamine abdominal injection, anesthesia is 4 ml / kg;

[0050]S2: After the anesthesia is improved to the sterile operator, the side rejection is fixed, and the hair is removed from the outer side of the femur to the outside of the mouse thigh, the elimination area is coincident with the femur, and the use of 1% vital iodine is 3 times. And wipe it with a sterile gauze;

[0051]S3: 0.8cm in the femur vertical axis, bluntly separated the subcutaneous film, the muscle gap between the straight muscle between the outer femoral muscle and the semicoprolaus is depolically separated, exposed the distal end of the femur (pay attention to protection when separating Inner strand artery located in the femur);

[0052]S4: At the nearly 1 / 3 of the femur using the teeth, the ophthalmic shear is held at a distal end of the femur and is ...

Example Embodiment

[0063]Example 3

[0064]The construction method of breast cancer bone metastasis in this embodiment includes the following steps:

[0065]S1: The 25 g of female mice were selected to give 1% ketamine abdominal injection, and the anesthesia was 4 ml / kg;

[0066]S2: After the anesthesia is improved, the sterile operator is moved, and the lateral rejection is fixed, and the hair is removed from the outer side of the femur to the outer side of the mouse thigh, the elimination area is combined with the femur, using 1% vital iodine disinfection 3 All over, and wipe it with a sterile gauze;

[0067]S3: 0.8cm in the femur vertical axis, bluntly separated the subcutaneous film, the muscle gap between the straight muscle between the outer femoral muscle and the semicoprolaus is depolically separated, exposed the distal end of the femur (pay attention to protection when separating Inner strand artery located in the femur);

[0068]S4: At the nearly 1 / 3 of the femur using the teeth, the ophthalmic shear is ...

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Abstract

The invention provides a breast cancer bone metastasis laboratory mouse disease model building method. The method comprises the following steps: selecting a mouse, anesthetizing the mouse, removing hair on the outer side of the thigh of the mouse, disinfecting the removed area, cutting open the skin of the mouse along the longitudinal axis of the femur, bluntly separating subcutaneous fascia, exposing the distal end of the femur, breaking the continuity of the femur of the mouse to form incomplete fracture, injecting tumor cells into the fracture part of the mouse, quickly closing the intermuscular space, washing the skin operation area of the mouse, injecting energy liquid into the abdominal cavity, and placing the mouse in a resuscitation box. According to the breast cancer bone metastasis laboratory mouse disease model building method provided by the invention, the tumor formation time is shortened by simulating bone tumor invasion in a bone dissolving state in a discontinuous fracture environment, and a bone marrow immune environment can be destroyed, so that immune escape of the tumor cells is realized, the attack of an immune system is avoided, tumors are formed, the survival rate of the tumors is increased, and the modeling success rate is increased.

Description

technical field [0001] The invention relates to the technical field of mouse model building, in particular to a method for building a breast cancer bone metastasis experimental mouse disease model. Background technique [0002] Breast cancer can spread to almost any area of ​​the body. The most common sites are lymph nodes, lung, bone, and liver. At present, breast cancer research is mainly based on breast cancer models established by rodents. In the field of clinical breast cancer bone metastasis, experimental animals are often used to make breast cancer bone metastasis models. The existing disease model construction is roughly divided into three methods. : The first is transplantation, that is, implanting tumor cells in the subcutaneous or muscle tissue. This method is not convincing because it lacks the same physiological environment as the clinical lesion; the second is injection in the mammary gland area of ​​experimental animals. Wait for its spontaneous bone metasta...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61D7/00
CPCA61D1/00A61D7/00
Inventor 闫晨晨刘国辉米博斌熊元李卉
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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