An inducer for establishing an animal model of rheumatoid arthritis and its preparation and application
A rheumatoid and animal model technology, applied in the biological field, can solve the problems of imperfect models, poor stability, poor reliability, and limited immune response, and achieve the goal of strengthening immune response, good stability and reliability, and high success rate of modeling Effect
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Embodiment 1
[0044] Example 1 Preparation of inducer (preparation process such as Figure 5 shown)
[0045] 1) Preparation of glacial acetic acid solution: take glacial acetic acid, add double distilled water, and dilute it into a glacial acetic acid solution with a concentration of 0.1mol / L;
[0046] 2) Preparation of collagen solution: add type II collagen to the glacial acetic acid solution prepared in step 1), blow it evenly, and store it in a refrigerator at 4°C for more than 8 hours to obtain a type II collagen solution. The type II collagen can be Type II collagen commonly used in cattle, pigs, humans, etc.;
[0047] 3) Prepare collagen emulsion: add the type II collagen solution prepared in step 2) dropwise to the same volume of complete Freund's adjuvant (as an optional way, the temperature of the complete Freund's adjuvant is controlled at 4-8°C) At the same time, add aluminum hydroxide, stir quickly to make it fully emulsified, and obtain a mixed emulsion containing type II ...
Embodiment 2
[0048] Example 2 Preparation of inducer
[0049] 1) Preparation of glacial acetic acid solution: take glacial acetic acid, add double distilled water, and dilute it into a glacial acetic acid solution with a concentration of 0.1mol / L;
[0050] 2) Preparation of collagen solution: add type II collagen to the glacial acetic acid solution prepared in step 1), and prepare a type II collagen solution with a concentration of 4 mg / ml collagen solution. After blowing evenly, store in a refrigerator at 4°C for 8 hours ;
[0051] 3) Collagen emulsion preparation: Add the type II collagen solution prepared in step 2) dropwise into the same volume of complete Freund's adjuvant (as an optional way, the temperature of the complete Freund's adjuvant is controlled at about 4°C) At the same time, add aluminum hydroxide, stir quickly to make it fully emulsified, and obtain a mixed emulsion containing type II collagen, aluminum hydroxide, glacial acetic acid and complete Freund's adjuvant, wh...
Embodiment 3
[0052] Example 3 Preparation of inducer
[0053] 1) Preparation of glacial acetic acid solution: take glacial acetic acid, add double distilled water, and dilute it into a glacial acetic acid solution with a concentration of 0.1mol / L;
[0054] 2) Preparation of collagen solution: add type II collagen to the glacial acetic acid solution prepared in step 1), and prepare a type II collagen solution with a concentration of 8 mg / ml collagen solution. After blowing evenly, store in a refrigerator at 4°C for 12 hours ;
[0055] 3) Collagen emulsion preparation: Add the type II collagen solution prepared in step 2) dropwise into the same volume of complete Freund's adjuvant (as an optional way, the temperature of the complete Freund's adjuvant is controlled at about 4°C) At the same time, add aluminum hydroxide, stir quickly to make it fully emulsified, and obtain a mixed emulsion containing type II collagen, aluminum hydroxide, glacial acetic acid and complete Freund's adjuvant, w...
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