Mouse cerebral ischemia reperfusion model optimization and failure assessment method

Abstract

The invention belongs to the technical field of mouse cerebral ischemia reperfusion models and discloses a mouse cerebral ischemia reperfusion model optimization and failure assessment method. A mousecerebral ischemia reperfusion model optimization and failure assessment system comprises a video monitoring module, a time module, a master control module, an anesthesia module, a sterilization module, a cutting module, an insertion module, a pulling-out module, a cerebral blood vessel three-dimensional structure module, a symptom monitoring module and a display module. By the cerebral blood vessel three-dimensional structure module for displaying cerebral blood vessel conditions after mouse cerebral ischemia reperfusion, effectiveness in division of cerebral blood vessel thick branches can be achieved, and cerebral blood vessel tiny structures can be extracted accurately. In addition, by intraoperative monitoring of vital signs including the body temperature, pulse, breath and blood pressure, the mouse survival rate is increased; by the postsurgical symptom monitoring module, quantitative assessment of neurological function damages after mouse cerebral ischemia reperfusion can be given, and important significance to cerebral arterial thrombosis prevention, curative effect assessment and the like is achieved.

Description

technical field [0001] The invention belongs to the technical field of mouse cerebral ischemia-reperfusion models, in particular to a method for optimizing and evaluating the results of mouse cerebral ischemia-reperfusion models. Background technique [0002] Cerebrovascular disease is a common clinical disease with high disability rate and high mortality rate. Among them, ischemic cerebrovascular disease is the most common case, and it is also a key research and urgent problem to be solved by domestic and foreign medical experts and scientific researchers. The current method is to explore new treatment methods through animal experiments, that is to say, through animal experiments, a model similar to human diseases is established in animals, in an attempt to find a solution to deep-seated treatment of cerebrovascular diseases. . Mouse cerebral ischemia-reperfusion model In the neck of the rat, the carotid common carotid artery is cut a small opening, and then a thread plug ...

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Problems solved by technology

[0004] (1) The current mouse cerebral ischemia-reperfusion model cannot accurately monitor the vital signs and ischemic area of ​​the mouse; the postoperative cerebral vascular structure cannot be accurately extracted; at the same time, the postoperative monitoring is single, and the evaluation of neurological impairment is inaccurate

[0005] (2) In the process of real-time monitoring of the process status of the mouse cerebral ischemia-reperfusion model through the camera in the prior art, due to the influence of the acquisition, transmission and processing delay of the image signal, the target cannot be at the best observation position of the camera , resulting in missing data, resulting in a lag in the analysis results, resulting in a large error in camera motion control

[0006] (3) The traditional thread embolization method is used in the prior art, which has a low success rate of modeling, increases intraoperative bleeding, and the thread knot is easy to fall off

[0007] (4) In the process of using infrared thermal imaging equipment to monitor the intraoperative cerebral ischemic area of ​​each mouse in the prior art, the outline of the cerebral ischemic area is incomplete and blurred

[0008] (5) No separation of cerebrovascular and background noise processing in the prior art, which is not conducive to the clear and correct display of cerebrovascular conditions after cerebral ischemia-reperfusion in mice

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