New method for screening anti-liver injury medicament by using model organism zebra fish

Abstract

The invention discloses a new method for screening an anti-liver injury medicament by using a model organism zebra fish. The method comprises the following steps: continuously molding the zebra fish serving as a molding object for 3 days by using preferred carbon tetrachloride at the concentration of 1mmol/L to obtain the zebra fish of a liver injury model, and then evaluating the anti-liver injury activity of different receptor medicaments by using ALT and AST levels in the liver tissue of the zebra fish and the pathological change condition of the liver tissue of the zebra fish. Experimental results show that the new method for screening the anti-liver injury medicament by using the model organism zebra fish has high molding success rate, can simulate an in vivo environment to perform quick screening, and has the advantages of strong operability, accurate experimental results, little amount of required reacceptance medicament, strong repeatability, low cost, wide application range, capability of performing batch screening experiments and high work efficiency.

Description

A new method for screening anti-liver injury drugs using the model organism zebrafish technical field The invention relates to a drug screening method, in particular to a new method for quickly screening anti-liver damage drugs by using the model organism zebrafish. Background technique Anti-liver injury drug screening research often includes in vivo (animal) and in vitro (cell, molecular, etc.) tests. In vivo anti-hepatic injury tests are often carried out with mice, rats and other animals. The main liver injury models include acute liver injury models and chronic liver injury models. Among them, most of the rapid screening of anti-liver injury drugs currently uses mouse models of acute liver injury. However, due to the large amount of drugs required for in vivo experiments in mice and the high cost of experiments, it is not conducive to the rapid screening of a small amount of drugs, especially those extracted from traditional Chinese medicines. The amount of the monome...

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Problems solved by technology

For example, Chinese patent 200810019040.5 discloses a method of using zebrafish to study drug toxicity, patent 200780051025.2 discloses a method of organ formation through stem cell nutrition and regeneration of alcohol-damaged organs, and 200310108710.8 discloses the use of zebrafish gene therapy for paraplegia Zebrafish has become a popular pharmaceutical research tool at home and abroad. At present, zebrafish research is mainly limited to developmental genetics, and has not been applied in the research of anti-liver damage drugs.

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