Manufacturing method of biological tooth root bracket material

A technology of scaffold material and construction method, which is applied in dentistry, dental implantation, dental prosthesis, etc., can solve the problem of alveolar bone regeneration without corresponding discussion, the reconstruction of biological root scaffold materials, and the inability to design scaffold materials, etc. problem, to achieve the effect of easy to master the construction operation, solve the problem of inducing the microenvironment, and low cost

Active Publication Date: 2011-05-25
成都世联康健生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the dentin matrix prepared in this study is mouse dentin, which is different from the dentin of other species such as humans. The constructed tissue is dentin, which cannot be designed as a root-shaped scaffold material. Moreover, biological tooth root materials are in the The important bioactive substances in the dentin matrix should not be destroyed to ensure that these bioactive substances...

Method used

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  • Manufacturing method of biological tooth root bracket material
  • Manufacturing method of biological tooth root bracket material
  • Manufacturing method of biological tooth root bracket material

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1, Preparation of human anterior tooth biological root scaffold materials and induced microenvironment:

[0035] The preparation method of the biological tooth root support material that the present embodiment enumerates comprises the following steps:

[0036] (1) Take four healthy front teeth, wash them three times with PBS solution, store them in culture bottles or other containers containing PBS, seal the bottles with parafilm and store them in a refrigerator at 4°C;

[0037] (2) Install corundum chips on a dental slow-speed turbine with a water spray device, adjust the speed to 3000 rpm, use corundum chips to grind off the crown of the tooth obtained in step (1), keep the root part and store it in a container containing In a PBS culture bottle or other vessel, seal the bottle with parafilm and store in a refrigerator at 4°C;

[0038] (3) Use a dental pulp extraction needle to completely extract the pulp tissue in the root of the tooth obtained in step ...

Embodiment 2

[0048] Embodiment 2, Preparation of human premolar biological root scaffold materials and induced microenvironment:

[0049] The preparation method of the biological tooth root support material that the present embodiment enumerates comprises the following steps:

[0050] (1) Take four premolars, wash them three times with PBS, store them in culture bottles or other containers containing PBS, seal the bottles with parafilm and store them in a refrigerator at 4°C.

[0051] (2) In the case of a water spray device, install emery chips on the slow-speed dental turbine, adjust the speed to 6500 rpm, use emery chips to remove the crown part of step (1), keep the root part and store it in a container containing In culture flasks or other vessels with PBS, seal the bottle with parafilm and store in a 4°C refrigerator.

[0052] (3) Use a dental pulp extraction needle to completely extract the pulp tissue in the root of the tooth obtained in step (2), and rinse the inside of the root...

Embodiment 3

[0061] Embodiment 3, Preparation of biological root scaffold materials and induced microenvironment for human molars (multi-rooted teeth):

[0062] The preparation method of the biological tooth root support material that the present embodiment enumerates comprises the following steps:

[0063] (1) Take four healthy molars, wash them three times with PBS solution, store them in culture bottles or other containers containing PBS, seal the bottles with parafilm and store them in a refrigerator at 4°C;

[0064] (2) Install corundum chips on a dental slow-speed turbine with a water spray device, adjust the speed to 4000 rpm, use corundum chips to grind off the crown part of the tooth obtained in step (1), keep the root part and store it in a container containing In a PBS culture bottle or other vessel, seal the bottle with parafilm and store in a refrigerator at 4°C;

[0065] (3) Use a dental pulp extraction needle to completely extract the pulp tissue in the root of the tooth ...

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Abstract

The invention discloses a manufacturing method of a biological tooth root bracket material. The method comprises the following steps of: flushing teeth with phosphate buffered saline (PBS) in a sterile state for three times; removing dental crown parts by grinding with a carborundum disc, and keeping tooth root parts; pulling pulp tissues out of the tooth roots and repeatedly flushing the inside of root canals with physiological saline; gradually expanding the inside of teeth with a dental expander and repeatedly flushing the inside of the root canals with 5 to 17 percent of ethylene diamine tetraacetic acid (EDTA) solution; grinding outer side tissues of the tooth roots by grinding with a dental turbine and constructing a biological tooth root profile as required so as to obtain a bracket with a specific shape; preserving the teeth in the PBS and treating in an ultrasonic oscillator for 3 to 8 times; performing stepped demineralization on a dentine with EDTA with different concentrations in a magnetic stirrer with the rotating speed of between 100 and 500 revolutions per minute so as to obtain a dentine substrate; and preserving the dentine substrate in double-antibody-containing PBS. The biological tooth root bracket material has a tooth root profile, the thickness of 1mm to 3mm, the length of 1cm to 2.5cm and certain tooth inducing capability, comprises a large number of proteins and factors related to the manufacturing of teeth, can release the proteins and factors continuously, can better solve the problem of induction micro-environment, and contributes to application to tooth socket intra-alveolar orthotopic transplantation.

Description

technical field [0001] The invention discloses a construction method of a biological tooth root bracket material. Background technique [0002] At present, the prevalence of tooth loss due to various reasons increases with age, and the prevalence is about 80% in middle-aged and elderly people. Tooth loss and abnormal tooth development seriously hinder the patient's chewing, language and other functions, and cause the patient's appearance deformity, cognitive defects, and psychological disorders, which greatly endanger the patient's physical and mental health. Therefore, it is urgent and arduous to seek effective treatment. task. [0003] At present, the missing teeth are all repaired by artificial prostheses, which have defects such as limited recovery of physiological functions, short service life, damage to normal tissues, etc., and lack of real physiological restoration. Restoration of missing teeth with implants is currently the focus and focus of the treatment of miss...

Claims

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Application Information

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IPC IPC(8): A61C8/00A61L27/36
Inventor 郭维华田卫东李锐杨波盛磊陈岗
Owner 成都世联康健生物科技有限公司
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