The invention provides a vascularized
skin flap and a preparation method thereof, and belongs to the field of
tissue engineering materials, and the preparation method of the vascularized
skin flap comprises the step of providing muscular tissue and artificial blood vessels. The muscular tissue is cooled, the cooled muscular tissue is subjected to
trepanning treatment, and the first structure is obtained. And performing contraction treatment on the artificial
blood vessel, and implanting the contracted artificial
blood vessel into the opening of the first structure to obtain a second structure. And performing expansion treatment on the artificial
blood vessel in the second structure to obtain a third structure. And performing
decellularization treatment on the third structure to obtain the vascularized
skin flap. Wherein the outer
diameter of the artificial blood vessel is larger than the inner
diameter of the opening. According to the technical scheme, the connection stability of the artificial blood vessel and muscular tissue can be improved, the ingrowth of new blood vessels and the vascularization process of vascularized flaps can be further promoted, the
survival rate of flap
transplantation is increased, and therefore the vascularization effect of the vascularized flaps is improved.