57 results about "Live animal" patented technology

The application provides a drug hepatotoxicity prediction method, device, system and medium, the method comprising: sequencing human liver cells after being contacted with a drug to be predicted to obtain a genome expression profile of the human liver cells after being contacted with the drug to be predicted; calculating the sum of the absolute values of the fold changes of the genes in the current gene pathway as the toxicity score of the current gene pathway according to the expression profile; calculating the Z value of the current gene pathway according to the toxicity score of the current gene pathway, the average value and the standard deviation value of the toxicity scores of the gene pathways calculated multiple times by a plurality of preset gene pathways; and predicting that the drug to be predicted has hepatotoxicity when the Z value is greater than 1.5. Therefore, the application can accurately, timely, effectively and sensitively predict drug hepatotoxicity without a large number of living animals as an experimental basis, and has good advantages in safety, environmental protection and animal protection. The application has the advantages of short experimental period and low cost.

The invention discloses a living body screening method of a high-throughput addiction inhibitor, which comprises the following steps: acquiring addictive nematodes with a plurality of addiction behavioral phenotypes; applying different addiction inhibitors to the addictive nematodes; the screening of the addiction inhibitors is realized according to the inhibition efficacy of the addiction inhibitors on the addiction behavioral phenotypes of the addiction nematodes, and based on the steps, the drug screening of the addiction inhibitors can be realized through the inhibition efficacy of the addiction inhibitors on the addiction behavioral phenotypes of the addiction nematodes in a high-throughput manner. Compared with the prior art, the medicine screening method of a traditional cell function system is abandoned, and medicine screening is carried out on the living animal behavioristics level in a brand new mode. Meanwhile, the advantages of easiness in large-scale culture, abundant behavioral modes and the like of the nematodes are utilized, and compared with expensive mammal feeding and experimental operation for drug screening, the method has higher cost performance, practical operability and high throughput.

A sensor (e.g., an optical sensor) that may be implanted within a living animal (e.g., a human) and may be used to measure an analyte (e.g., glucose or oxygen) in a medium (e.g., interstitial fluid, blood, or intraperitoneal fluid) within the animal. The sensor may include a sensor housing, an analyte indicator covering at least a portion of the sensor housing, and one or more compounds containing boronate or boronic acid moieties that reduce degradation of the analyte indicator.

Provided herein are compositions and methods for identifying effective delivery vehicles (DVs) for mRNA delivery to and expression in specific cells or tissues in culture (in vitro) or in live animals (in vivo) from a pool of co-administered DVs.

The embodiment of the invention relates to the technical field of vehicles, and discloses a living object expelling method and device and a vehicle. The living object expelling method comprises the steps that whether a target living object exists in a vehicle blind area or not is determined based on detection data of the vehicle blind area; wherein the detection data of the vehicle blind area is used for reflecting the existence condition of living things in the vehicle blind area; the vehicle blind area refers to an area outside a driver view area and in a vehicle driving track, and the driver view area refers to a sight coverage area when a driver is in a driving position; under the condition that the target living object exists in the blind area of the vehicle, a target expelling strategy for the target living object is determined based on the living object type of the target living object and the area type of the area where the vehicle is located currently; and controlling the vehicle to execute the target expelling strategy. According to the technical scheme, the living body targets in the driving blind area can be repelled, and safety accidents are reduced.

This invention relates to the field of leukemia research, specifically disclosing an animal model of CAR-T therapy for leukemia complicated by cytokine release syndrome (CRS), its preparation method, and its application. The method for preparing the animal model of CAR-T therapy for leukemia complicated by CRS includes: obtaining CD19 CAR-T cells: peripheral blood mononuclear cell isolation, T cell magnetic bead sorting, T cell activation, lentiviral infection, and CAR-T cell expansion; constructing an animal model of CRS after high- and low-dose CAR-T infusion: implanting Nalm-6 cell line into living animals to induce leukemia tumor burden, obtaining living animals with leukemia tumor burden, and injecting CD19 CAR-T cell line into the living animals with leukemia tumor burden. This invention provides a novel application for the animal model of CAR-T therapy for leukemia complicated by cytokine release syndrome. By using severely combined immunodeficient mice and inoculating them with high- and low-burden tumor cells, and infusing different doses of CAR-T cells, this invention constructs an in vivo model that can simulate clinical CRS, providing research guidance for clinically exploring more effective interventions.

The utility model discloses a small animal test post-operation wound surface shooting device, which comprises a base, a support vertical rod and an object placing table, the object placing table is arranged on the upper surface of the base, the support vertical rod is vertically fixed on the base, the support vertical rod is arranged on one side of the object placing table, a support arm assembly is arranged on the support vertical rod, and the support arm assembly is connected with the object placing table. Meanwhile, a clamping head assembly is arranged on the supporting arm assembly, a shooting instrument is clamped by the clamping head assembly, a fixing table assembly is installed on the object placing table, the device achieves effective constraint on shot living animals, the scale assembly is matched with the supporting vertical rod, the supporting arm assembly and the clamping head assembly, it is ensured that the proportions of images shot by different devices at different times are consistent, and the shooting efficiency is improved. The scale is used as a correction reference, the actual area of the wound surface can be automatically converted in software, the manual measurement error is reduced, the fixing table assembly and the fixing rod are designed in a modularized mode, 20-500 g experimental animals can be compatible, and the limitation of one mouse and one device of a traditional device is overcome.

A sensor (e.g., an analyte sensor) that may be implanted partially or fully within a living animal (e.g., a human) and may be used to measure an analyte (e.g., glucose or oxygen) in a medium (e.g., interstitial fluid, blood, or intraperitoneal fluid) within the animal. The sensor may include a housing, a first polymer that includes analyte indicator molecules and covers at least a portion of the housing, and a second polymer that forms an interpenetrating network with the first polymer. The second polymer may prevent biological or protein interference of the sensor.

The invention provides a near-infrared fluorescence activated protein, a gene containing the near-infrared fluorescence activated protein, an expression box and a using method thereof, and a cell and a kit for generating the near-infrared fluorescence activated protein. A compound formed after the near-infrared fluorescence activated protein is combined with a corresponding fluorescent ligand has strong fluorescence, the fluorescence excitation peak wavelength is 574nm, near-infrared light with the emission peak wavelength is 680nm, and the near-infrared fluorescence activated protein has the advantages of high fluorescence quantum yield, large two-photon absorption cross section and the like, and has the advantage of high signal-to-noise ratio in vitro, living cells and living animals.

The application discloses a safety detonation method of electronic detonator, which comprises the following steps: using a UAV to perform safety identification and confirmation on a blasting danger area; if the UAV finds that the blasting danger area has personnel or live animals, a driving-off program is started; after a detonation controller completes network inspection of the electronic detonator and transmits a delay command of the electronic detonator, the UAV sends a safety confirmation instruction to a detonator; after the detonation controller receives the safety confirmation instruction, the detonation controller is unlocked and charges and detonates the electronic detonator; after the UAV sends the safety confirmation instruction, the blasting danger area is still monitored to determine whether to perform a blasting interruption operation; and the core of the method is that the detonation controller can be unlocked and charges and detonates the electronic detonator only when it is confirmed that the blasting danger area is safe, so that the deficiency of artificial safety confirmation is made up, and the possibility that a blasting safety accident occurs due to human negligence or the difficulty of confirming safety of personnel in a complex scene is prevented.

The utility model relates to the field of animal transport equipment, in particular to a living animal transport vehicle, at least two placement frames are arranged in a transport cabin of the living animal transport vehicle at intervals, each placement frame comprises at least one placement plate, and the placement plates are arranged in the horizontal direction and divide the interior of the transport cabin into a plurality of placement layers in the vertical direction; the mounting position of the placement plate on the placement frame is adjustable in the vertical direction; for animals which are small in size and suitable for being caged, the height of the placement layers can be reduced, animal cages can be placed on the placement frames as many as possible, and the placement frames are arranged at intervals, so that workers can walk and assemble and disassemble the animal cages conveniently; for animals which are large in size and not suitable for being caged, the placement frame in the transportation cabin can be detached, so that the animals are loaded in the transportation cabin, the placement frame is adjusted according to factors such as the size habits of the animals, the number of the animals capable of being loaded in the transportation cabin can be increased as much as possible, the transportation efficiency is improved, and the cost is reduced.

Provided herein are compositions and methods for identifying, from a co-administered DV pool, an effective delivery vehicle (DV) for delivery and expression of mRNA to and in a particular cell or tissue in a culture (in vitro) or in a living animal (in vivo).

The present invention relates to a smart pet collar for tracking the location of live animals, collecting their health data, and providing a warning in the event of the animal being stolen.

An animal transportation system forms a climate-controlled environment that includes one or more cage assemblies configured to accommodate one or more live animals to be transported therein. The one or more cage assemblies comprise a covering extending thereabove, a frame having a support laterally extending therefrom, and one or more doors supported spaced apart from the one or more cages by the support and movable between an open position and a closed position. One or more securement systems extend through an opening in a floor of the animal transportation system and flexibly secure the one or more cage assemblies to the floor. A circulation system is configured to affect environmental conditions of an interior of the animal transportation system. A control system may be coupled to the circulation system.

The invention discloses a construction method and an application of Elizabeth mileli for expressing Nanoluc luciferase. The Elizabeth mileli can be used for expressing Nanoluc luciferase. The coding gene of the Nanoluc luciferase is knocked into the genome of the Elizabeth mileli FL160902 strain in a fixed-point manner through a homologous recombination technology, so that the report strain capable of stably expressing the luciferase is successfully constructed. The reporter strain has normal growth characteristics, and the in-vitro detection sensitivity can reach 100 CFU. By utilizing a xenopus laevis infection model, the real-time dynamic tracing of the report strain in a living animal body is realized for the first time. The Nluc report strain constructed by the invention has the characteristics of strong luminescence stability, high detection sensitivity and the like, and provides a high-sensitivity visual research tool for analyzing the infection dynamic state and pathogenic mechanism of Elizabeth mileli.

A sensor (e.g., an optical sensor) that can be implanted within a living animal (e.g., a human) and used to measure an analyte (e.g., glucose or oxygen) in a medium (e.g., interstitial fluid, blood, or intraperitoneal fluid) within the animal, the sensor including a sensor housing, an analyte indicator covering at least a portion of the sensor housing, and a multi-metal protection system including multiple metals incorporated within and / or proximate to a surface of the analyte indicator that reduces deterioration of the analyte indicator.

The invention relates to an amyotrophic lateral sclerosis zebrafish model based on beta-methylamino-L-alanine induction as well as a construction method and application of the amyotrophic lateral sclerosis zebrafish model. According to the method for constructing the amyotrophic lateral sclerosis model by inducing the zebrafish through the BMAA, the non-specific fatality rate in the early stage of modeling is effectively reduced by limiting the initial development node and culture conditions of toxin exposure, and the living animal model with typical motor nerve injury phenotype and good repeatability is obtained. The method is short in construction period and relatively low in experiment cost, can objectively reflect sporadic ALS pathological characteristics induced by environmental factors, and is suitable for being used as an in-vivo biological carrier for high-throughput preliminary screening of anti-ALS drugs and related neurotoxicological researches.

The invention relates to the technical field of optical molecular imaging, and particularly discloses a small animal three-dimensional fast tomography method which comprises the following steps: S1, firstly, putting a living body gathering animal on the upper surface of an electric rotating platform for vertical fixation, and then fixing an optical CCD camera and a fluorescent CCD camera which are aligned with the height center line of the living body gathering animal; an optical CCD camera and a fluorescent CCD camera are fixed and connected with a computer, and an electric rotating platform which accurately rotates for thirty-six times is matched to drive a focused living animal to accurately rotate, so that the optical CCD camera and the fluorescent CCD camera can quickly shoot every time the focused living animal rotates, and the accuracy of the focused living animal is improved. After shooting for thirty-six times, synthesizing an optical three-dimensional model and a fluorescent three-dimensional model through computer software, and then rotating one of the three-dimensional models to coincide, thereby completing the most accurate and rapid three-dimensional fluorescent tomography work.

This invention discloses a metal-free, highly sensitive, rapid fluorescent probe, its preparation method, and its application in the detection of pathogenic bacteria in food, belonging to the field of food detection technology. The probe consists of an organic fluorescent molecular graphene composite matrix, an organic-inorganic hybrid dopant, and a specific recognition molecule. The dopant is carboxylated cellulose and silica nanoparticles, with a mass ratio of 1:6~10 to the composite matrix. The specific recognition molecule is a pathogenic bacteria aptamer, grafted onto the probe surface via spray coating. The probe triggers a fluorescence response by the specific binding of the aptamer to the target pathogenic bacteria, completing detection within 5 minutes with a sensitivity ≥90% and a specificity ≥98%. The probe contains no metal components, posing no toxicity or environmental risk, and is suitable for rapid on-site food detection and live animal detection. Simultaneously, the preparation cost is reduced by 90%, and the cost per sample is reduced by 40%, demonstrating promising application prospects.

The invention relates to the technical field of biology, in particular to a construction method and application of a helicobacter pylori luciferase bacterial strain, the helicobacter pylori luciferase bacterial strain is obtained by introducing a Nanoluc gene into a helicobacter pylori genome, and the bacterial strain can efficiently express luciferase. The fluorescent probe can interact with a synthetic substrate furimazine to initiate lasting and bright bioluminescence, can be directly used for living body imaging, and is used for animal model construction and vaccine and therapeutic drug evaluation in the helicobacter pylori vaccine research and development process. Animal experiments prove that the helicobacter pylori luciferase strain interacts with furazine in the stomach of a living animal to emit bright and lasting bioluminescence, and is used for observing sustainable infection of the living animal. The method lays a foundation for further evaluation of helicobacter pylori vaccine protectiveness, and plays an important role in development and application of prevention and treatment vaccines and drugs.

The invention relates to a weight detection method and device for an animal living body and electronic equipment. The method comprises the steps that weight detection data of a target object on target equipment is acquired, and the target object has a dynamic behavior causing weight detection fluctuation on the target equipment; carrying out filtering processing on the weight detection data; caching the filtered processing data, and calculating distribution parameters of the processing data when the processing data reaches a preset number; under the condition that the distribution parameters meet the parameter threshold value of the static state, the detection weight is calculated according to the preset number of processing data. The problem that the accuracy of weight detection is low due to fluctuation of weight detection caused by dynamic behaviors of a pet when a weighing device directly weighs the pet in the related technology is solved.

This invention discloses a dynamic post-loan monitoring and risk warning system and method for live animal collateral, belonging to the interdisciplinary fields of financial technology and smart animal husbandry. The system establishes a unique digital identity for collateral assets through an identity trust anchoring module using animal iris as the root of trust; dynamically collects quantity, health, location, and morphological data through a multimodal data sensing network; performs data fusion and preliminary risk assessment locally through an edge computing gateway with collaborative triggering and verification capabilities; accurately identifies and provides early warnings for risks such as health deterioration, abnormal asset reduction, and abnormal movement through a cloud-based intelligent risk control engine based on time-series machine learning; and immutably stores key business data through a blockchain trusted evidence storage module. When a high-risk event is identified, it can automatically trigger warnings and even asset freezing instructions through the financial institution's API interface. This invention solves the core risk control problem of the difficulty in using live animal assets as qualified collateral.

The application discloses a luciferase substrate, a preparation method and use thereof. The luciferase substrate has the advantages of long emission wavelength, high light intensity and high detection sensitivity after acting on firefly luciferase, and has good performance in live cell and live animal imaging, and can be used as an important analysis tool in the fields of live bio-luminescence imaging and protein quantitative detection.

To provide a halal-compliant disinfectant solution. [Solution] This disclosure provides a disinfectant solution for disinfecting animals. This disclosure provides a disinfectant solution comprising chlorous acid water, which disinfects an animal by direct contact of the disinfectant solution with the animal. The contact is achieved by rubbing, scrubbing, or basin. The animal is a living animal. The animal includes the surface of the animal. The surface of the animal is skin. The surface of the animal is a finger. The animal includes the human body. The disinfection is performed in the preparation of food. The disinfectant solution does not contain animal-derived components, alcohol components, or fragrances.

The invention provides a method for screening lysosomal storage disease related genes based on a caenorhabditis elegans lysosomal overload model and application of the method. The method comprises the following steps: constructing a transgenic nematode strain containing an intestinal specific promoter nhx-2p-driven CPL-1 and red fluorescent protein wrmScarlet fusion expression vector; intestinal cells are induced to secrete CPL-1:: wrmScarlet fusion protein into coelomic fluid, and the fusion protein is endocytosed and accumulated in lysosome by coelomic cells to form a quantifiable overload phenotype, and then gene expression is knocked down by adopting a feeding RNA interference technology; the red fluorescence signal intensity change of body cavity cells is observed under a stereoscopic fluorescence microscope so as to screen candidate genes for promoting or relieving lysosome storage, and meanwhile, the model can be used for drug screening, and has the advantages of direct observation through a low-power lens at a living animal level, high flux, quantifiability, convenience in operation and the like; and the long-standing key technical bottleneck in the field of lysosomal storage diseases is solved.

The invention is suitable for the technical field of living animal logistics order management, and provides a logistics order management method and system, and the method comprises the steps: obtaining the real-time monitoring videos of a plurality of living animals in a target logistics transportation box, and obtaining the active degree broken line of each living animal through the real-time monitoring videos; in the transportation process of the logistics transportation box loaded with the living animals, whether the living environment of the animals in the logistics transportation box is suitable for the living animals or not can be intelligently and timely known, and when it is determined that the living environment of the animals in the target logistics transportation box is not suitable any more, the living animals can be timely detected. According to the method and the device, the reasons for causing the animal living environment in the logistics transportation box to be inappropriate can be objectively determined by performing different-dimension environment regulation measure trial on the animal living environment in the logistics transportation box, and finally the animal living environment in the logistics transportation box returns to be appropriate according to the inappropriate reasons. Therefore, the intelligent degree of the living animal logistics order management method is improved.

The invention discloses a cross-border diarrhea pathogenic bacterium multi-source data fusion and early warning system, relates to the technical field of epidemic prevention, and aims to solve the problem that in the prior art, it is difficult to realize prospective early warning and accurate evaluation of pathogenic bacterium outbreak risks during cross-border transportation of living animals. The system comprises a multi-source data acquisition module, a data preprocessing module, a pre-stress score calculation module, a pathogenic bacterium outbreak association module and a risk comprehensive assessment module. The multi-source data acquisition module is used for acquiring full-process multi-dimensional data of cross-border living animal transportation, including physiological stress indexes, behavior stress indexes, transportation environment indexes and pathogenic bacteria indexes; the data preprocessing module is used for carrying out standardization processing on the collected full-process multi-dimensional data; and the pre-stress score calculation module obtains the comprehensive pre-stress score of the living animal by establishing a pre-stress scoring model. The method has the advantage of realizing prospective early warning and accurate quantitative evaluation of risks.

A sensor (e.g., an optical sensor) that may be implanted within a living animal (e.g., a human) and may be used to measure an analyte (e.g., glucose or oxygen) in a medium (e.g., interstitial fluid, blood, or intraperitoneal fluid) within the animal. The sensor may include a sensor housing, an analyte indicator covering at least a portion of the sensor housing, and one or more compounds containing boronate or boronic acid moieties that reduce degradation of the analyte indicator.

This application relates to the fields of intelligent measurement and machine vision technology, and discloses an intelligent sensing and measurement system for biological body length based on image recognition. The system includes modules for multimodal data acquisition, dynamic behavioral feature extraction, adaptive weight filtering, bidirectional collaborative locking based on apparent weight, and image length measurement and data fusion. It dynamically adjusts the filtering window based on the frequency of struggling to smooth physical impact noise, and combines the rate of weight change with body curvature for bidirectional collaborative locking. Based on pre-set species classification labels, it matches corresponding feature point detection strategies to achieve absolutely synchronous and accurate measurement of the weight and dimensions of multiple body parts of different species such as fish, shrimp, and crabs. Through adaptive filtering based on action frequency, bidirectional collaborative locking based on apparent weight, and density-based hardware linkage adjustment, the system automatically uploads time-aligned structured data packets directly to the computer system, eliminating manual recording and improving the accuracy and automation efficiency of dynamic live animal measurement.

This invention relates to a sensor (e.g., an optical sensor) that can be implanted in a living animal (e.g., a human) and used to determine analytes (e.g., glucose or oxygen) in intracellular media (e.g., interstitial fluid, blood, or peritoneal fluid). The sensor may include a sensor substrate, electrodes or a housing, an analyte indicator covering at least a portion of the sensor, and one or more probes for identifying degradation substances in the sensor environment.