Novel mouse in-situ pleural mesothelioma model and establishment method thereof

Abstract

The invention discloses a novel mouse in-situ pleural mesothelioma model and an establishment method thereof. The establishment method comprises the following steps of 1, taking 90 BLAB/C nude mice as an experimental group and 30 control groups, and uniformly putting the experimental group and the control groups into an incubator for normal culture; 2, under the condition that BLAB/C nude mice breathe, heartbeat and the like are normal, anesthetizing the mice and fixing the mice on an animal operating table in a supine position for experiment; and 3, at the intersection point of the left rib 5/6 intercostal space and the left chest wall anterior axillary line of the mouse, using microscopic forceps to lift and pull the skin at the intersection point, then using a 22G sterile sharp needle head to break the skin at the intersection point, then using a 30G sterile blunt pillow to break the wall layer pleura along the broken opening. The tumor transplantation method provided by the invention is applicable to orthotopic transplantation of pleural tumors. The growth mode, the invasion ability and the tumor metastasis effect of the pleural tumor can be restored to the maximum extent, and a good mouse model is constructed for studying the pleural tumor.

Description

technical field [0001] The invention belongs to the technical field of pleural tumor orthotopic transplantation, in particular to a novel mouse orthotopic pleural mesothelioma model and a method for establishing the same. Background technique [0002] Pleural mesothelioma is a primary tumor of the pleura, which originates from the visceral layer, parietal layer, mediastinum or the four parts of the diaphragm. The incidence in foreign countries is higher than that in China, 0.07-0.11% and 0.04% respectively. Mortality accounts for less than 1% of all tumors worldwide. It is more common in people over 50 years old, and the ratio of male to female is 2:1. The disease is associated with asbestos exposure. Malignant type is still lack of effective treatment. [0003] The pleura is the serosa covering the mediastinum on the inner surface of the chest wall of the left and right lungs. Medicine expresses the various functional activities of the zang-fu organs as "Qi". Some peopl...

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Problems solved by technology

[0005] The technical problem to be solved in the present invention is to overcome the existing defects, provide a novel mouse orthotopic pleural mesothelioma model and its establishment method, to solve the pleural mesothelioma tumorigenesis in the current animal experiments proposed in the above-mentioned background technology It is common to form tumors mainly in the axillary or abdominal wall, but the tumors formed by the above-mentioned tumor formation methods cannot simulate the impact of the tumor on the surrounding tissues (such as: visceral pleura, parietal pleura, adjacent lung tissue) during the growth process. Problems with damage and invasion effects

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