84 results about "Treatment and control groups" patented technology

The invention mainly aims at humanbody health assessment and personalized diagnosis of microbial flora relateddiseases, and proposes and validates an assessment and diagnosis method based on human body flora interaction network analysis. The method aims to detect the number of 15 kinds of special function nodes-containing triangle motifs (trios) in a flora interaction network, wherein there are two major categories including a branch with no special function nodes and a branch with function nodes, the branch with no special function nodes is divided in six typesaccording to positive and negative relations between three nodes, and the branch with the function nodes is divided in nine typesaccording to the number of interaction and positive and negative relations of the function nodes and the triangle motifs. The inventor finds that there is significant difference in the number of specific trios in the flora interaction network between experimental group/disease group and healthy controls by analyzing microbial flora data of six human body parts includingintestines, vaginas, lungs, mouths, semenand skin. The method provides an effective reference index forrisk assessment of humanbodyhealth and disease occurrence, and provides reliable technical support for the personalized diagnosis of the microbial flora relateddiseases.

The invention provides an experimental method based on the tensile stress lasting time serving as a variable, and relates to the field of impact dynamics. The experimental method includes S1, producing a plurality of flying sheets; S2, producing a plurality of sample targets, wherein each sample target comprises a first sample layer and a second sample layer, the first sample layers are thinner than the second sample layers, the total thicknesses and textures of the sample targets are the same, and the thicknesses of the first sample layers of the target samples are different; S3, corresponding each flying sheet to the sample targets to form an impact experiment group, wherein the sample targets in each impact experiment group are arranged adjacently; S4, performing impact experiments on the impact experiment groups. The experimental method has the advantages that the tensile stress lasting time is taken as the single variable successfully, intermediate experiment information and data in the same damage evolution path are acquired, and the influence of taking the tensile stress lasting time as the single variable on the slabbing phenomenon is predicted accurately.

The invention discloses a method for researching the effects of Dickkopf-1 and cell apoptosis in steroid-induced avascular necrosis of femoral head (SANFH). The method comprises the following steps: by taking the femoral head sample of SANFH taken through total hip replacement as an experiment group and taking the sample after the hip replacement for fracture of neck of femur as a contrast group, cutting the femoral head sample, fixing and decalcifying the cut femoral head sample to prepare slices, observing the morphologic change of osteocytes by an electronic microscope, observing the pathological change of the osteocytes by a light microscope, counting the vacant bone lacuna, detecting the expression of Dickkopf-1 by an immunohistochemical staining technique, thus exploring the effects of Dickkopf-1 and cell apoptosis in SANFH. The method can be used for providing assistance for early diagnosis and curing of SANFH and also providing a theoretical basis for clinical prevention and curing of SANFH in future.

The invention belongs to the technical field of cell secretion extraction, and discloses a method for extracting exosome derived from human hair follicle dermal papilla cells (DPC-Exos). The method isas below: identifying DPC-Exos by controlled resistance pulse sensing (TRPS) analysis, electron microscopy and Western Blot; and adding the DPC-Exos to a culture medium of human hair follicle outer root sheath cells (ORSCs) cultured in vitro. The DPC-Exos are identified to be about 105 nm in diameter and expressing tumor susceptibility gene (TSG) 101, differentiation cluster (CD) 9 and CD63; DPC-Exos promote proliferation and migration of human ORSCs, and mRNA and protein levels of beta-catenin and Sonichedgehog (Shh) in ORSCs are detected to be up-regulated after treatment; transdermal injection of DPC-Exos accelerates an HF growth phase of mice and delays the occurrence of regression. Immunohistochemical analysis shows that the expression levels of beta-catenin and Shh are up-regulatedin the hair follicles of an experimental group. DPC-Exos help regulate the growth and growth cycle of the HF and provide a new potential for the treatment of clinical hair loss.

The invention discloses a treating method for a viscous fluid specimen, and relates to the technical field of biochemistry. The method includes the steps that the viscous fluid specimen is added into pre-prepared treating agents with the volume equal to that of the viscous fluid specimen, and repetition is carried out to obtain experimental groups; the experimental groups are treated according to the detected type of the viscous fluid specimen to obtain viscous fluid specimen treating fluids, and then the viscous fluid specimen is treated. By means of the method, the liquefaction time of the specimen is significantly shortened, the viscosity of the specimen is reduced, and the diagnosis precision is not affected; the method can be flexibly applied according to diagnosis purposes; meanwhile, medical cost is not increased.

The invention discloses a disease-resistant immunoenhancement cyclo-(phenylalanine-serine) for channa argus, provides a composite feed for channa argus, in which pseudomonas putida metabolin cyclo-(phenylalanine-serine) is added so as to improve the disease-resistant ability of the channa argus and promote growth. After the channa argus is fed with the feed, through immunological detection, the serum lysozyme activity, serum phagocytosis ability and serum bactericidal ability of channa argus in an experimental group are obviously improved respectively up to 78.26U/mL, 63.52U/mL and 75.69U/mL, which are higher than those of a control group (respectively 17.23U/mL, 20.96U/mL and 39.36U/mL). Relative expression of IL-1beta gene of channa argus is up-regulated to 25.81, while relative expression of IL-1beta gene of channa argus in the control group is only 2.45; and by injecting medial lethal concentration of bacteria into fish belly to detect the disease resistance, the accumulative death rate of the experimental group after 15 days is 36% minimally, while that of the control group is 68%, and the survival rate of the experimental group is higher than that of the control group obviously. A comprehensive breeding method provided by the invention, matched with cultivation, makes full use of a water body, and an immunoenhancement is added, thus improving immunity and promoting growth, and enhancing economic benefits.

The invention discloses a screening method of a pH-dependent antibody targeting complement protein C5 and an antibody obtained by the screening method. The screening method disclosed by the inventionis carried out on a biomolecule interaction analysis system; the method comprises the following steps of: setting an experiment group 1, a control group 1, an experiment group 2 and a control group 2,and enabling each group of chips to be combined with antigen complement protein C5 (A) as a reference antibody (Ab1) and a to-be-detected antibody (Ab2) of a pH-dependent C5 monoclonal antibody: theexperiment group 1 A-Ab1-Ab2; the control group 1 is A-Ab1-0; the experimental group 2 is A-0-Ab2; the control group 2 is A-0-0; and detecting the binding signal and calculating the ratio of (experimental group 1-control group 1)/(experimental group 2-control group 2) to judge whether the antibody to be detected is a pH-dependent C5 antibody or not. According to the method, the pH-dependent C5 monoclonal antibody development period can be shortened, and the labor capacity and the time cost are saved; a hybridoma cell culture supernatant can be used as a to-be-detected sample for direct identification, so that the antibody screening efficiency is further improved.

The invention relates to a neutralizing antibody of follistatin-like 1 and an application thereof. The antibody is a murine FSTL1 neutralizing antibody 2K6. The invention also discloses a heavy chainvariable region and a light chain variable region of the antibody or an antigen binding fragment thereof. The invention also relates to an application of the FSTL1 in promoting the treatment of skin fibrosis diseases, the application of the murine FSTL1 neutralizing antibody 2K6 in treating or remitting pulmonary fibrosis, skin fibrosis and arthritis diseases or symptoms, and a pharmaceutical composition prepared from the murine FSTL1 neutralizing antibody 2K6. Research results show that the murine FSTL1 neutralizing antibody 2K6 can treat or remit pulmonary fibrosis, skin fibrosis and arthritis diseases; and compared with an antibody 22B6 which is researched and developed by the research and development team in the earlier stage and used for treating pulmonary fibrosis, the antibody 2K6 has better prevention and treatment effects on mouse pulmonary fibrosis than the antibody 22B6, that is to say, the prevention and treatment remission rates of a 2K6 experimental group on pulmonary fibrosis key indexes including the hydroxyproline content and the pulmonary fibrosis area proportion are higher than those of a 22B6 experimental group.

The invention discloses a novel mouse in-situ pleural mesothelioma model and an establishment method thereof. The establishment method comprises the following steps of 1, taking 90 BLAB/C nude mice as an experimental group and 30 control groups, and uniformly putting the experimental group and the control groups into an incubator for normal culture; 2, under the condition that BLAB/C nude mice breathe, heartbeat and the like are normal, anesthetizing the mice and fixing the mice on an animal operating table in a supine position for experiment; and 3, at the intersection point of the left rib 5/6 intercostal space and the left chest wall anterior axillary line of the mouse, using microscopic forceps to lift and pull the skin at the intersection point, then using a 22G sterile sharp needle head to break the skin at the intersection point, then using a 30G sterile blunt pillow to break the wall layer pleura along the broken opening. The tumor transplantation method provided by the invention is applicable to orthotopic transplantation of pleural tumors. The growth mode, the invasion ability and the tumor metastasis effect of the pleural tumor can be restored to the maximum extent, and a good mouse model is constructed for studying the pleural tumor.

The invention provides a new coronal pneumonia patient outcome prediction method based on an interpretable machine learning algorithm, wherein the method comprises the steps: extracting COVID-19 patient data from a database, and dividing the patient data into an experimental group and a control group according to the illness state conversion condition of a patient; interpolating the missing value of each index through random forest regression; screening the indexes of the input model, and taking the screened indexes as key risk factors for identifying the deterioration of the patient; inputting the key risk factors of the patient into the XGBoost model and the logistic regression model; selecting an XGBoost model with better prediction expressive force to generate an index combination, and performing prediction by using the XGBoost model and recording the prediction result; defining the early warning range of the key index; when the key risk index of the patient enters the early warning range, giving out an alarm prompt to medical staff. According to the invention, the calculation result of the algorithm and the clinical experience of a doctor are synthesized, and two index combinations composed of 15 first groups of indexes and 5 second groups of indexes are proposed to be used for predicting the condition of the new coronal pneumonia patient.

The invention relates to a method for detecting the fibrinolysis function of blood. The method comprises the following steps: a, preparing an experimental group reagent and a control group reagent; b,performing absorbance detection; and c, calculating the fibrinolysis capacity value and the fibrinolysis time value. The fibrinolysis function of blood can be directly detected; the method is relatively simple to operate; the fibrinolysis function of the blood can be quantitatively evaluated, namely the fibrinolysis capacity and the fibrinolysis time are reflected; and traditional fibrinolytic markers such as FDP and D dimers are high in non-specificity and affected by inflammation, bleeding, thrombus load and the like, and the real fibrinolytic ability can be reflected, so that the specificity is high. In addition, the method is not influenced by blood chyle, jaundice, hemolysis and other factors, and the anti-interference capability is high.

The invention provides an experimental sample allocation method and device, equipment and a computer readable storage medium, and aims to allocate a random code to each experimental sample and allocate the experimental samples to an experimental group according to the random codes of the experimental samples and a preset allocation rule. The random codes are randomly distributed, the upper limit of the sample proportion of the experimental group is based on experiments included in the experimental group and experiments included in other experimental groups in the experimental layer where the experimental group is located, and therefore the sample proportion upper limits of different experimental groups can be different; the random code is smaller than the upper limit of the preset sample proportion of the experimental group; different experimental samples can be allocated to different experimental groups, and in addition, the situation that the number of the experimental samples allocated by a single experimental group is larger than the actually required number of the experimental groups can be prevented as the proportion of the experimental samples allocated by the experimental groups is smaller than the upper limit of the sample proportion of the experimental groups. Therefore, according to the method disclosed in the technical scheme provided by the invention, the use efficiency of the experimental sample can be improved.

The invention relates to a difference analysis method based on single cell samples of a mixed experimental group and a control group, which comprises the following steps: a, acquiring data, and carrying out quality control and standardization processing on the data, the data comprising experimental data and open source data; b, screening out hypervariant gene data from the standardized data, and carrying out dimensionality reduction on the screened data; c, performing multiple rounds of clustering analysis of different parameters on the single cells according to the data subjected to dimension reduction, and calculating the composition proportion of a cell subset experimental group and a control group of each round of clustering analysis to obtain an optimal result; and d, carrying out difference analysis on the cell subpopulation. The invention further relates to a difference analysis system based on the single cell samples of the mixed experimental group and the control group. According to the invention, the cell subgroup formed by the specific group can be better found, so that the difference analysis of the experimental group and the control group at the single cell level is realized.

The invention discloses application of p38 gamma in preparation of a pancreatic cancer prognosis diagnostic reagent. The inventor finds that expression of p38 gamma in cancer tissues of a pancreatic cancer patient is remarkably higher than that of p38 gamma in normal tissues, the expression level of p38 gamma is gradually increased along with disease progression, and the high expression of p38 gamma is related to adverse prognosis of the patient. A pancreatic duct adenocarcinoma animal model K-RasG12D-p53R172H-Pdx-Cre (KPC) is constructed as a control group for research. A pancreatic ductal epithelial cell p38 gamma knockout pancreatic cancer animal model (KPC-p38 gamma-KO) is constructed as an experimental group by applying a Cre/LoxP technology. The p38 gamma knockout is found to be capable of remarkably reducing the size of a tumor and prolonging the lifetime of KPC mice. The median survival time of the mice in the KPC group is 160 days, most KPC-p38 gamma-KO mice still survive in the statistical time, and the median survival time is longer than 300 days. A further experimental result shows that high expression of p38 gamma can promote invasion and metastasis of a pancreatic cancer. Therefore, p38 gamma can well determine the risk and prognosis of the pancreatic cancer.

The invention belongs to the technical field of biology, and particularly relates to a molecular marker related to senescence and health senescence and application of the molecular marker in improvinghealth senescence. According to the invention, Tai Chi exercisers are used as an experimental group, protein spectra are compared with those of normal young people and old people, the FABP4 protein is screened out, the expression of the FABP4 protein in blood of old people is significantly increased compared with that of young people, but the trend of the FABP4 protein in Tai Chi exercises of oldpeople is significantly reversed. The FABP4 gene expression is knocked down or the FABP4 activity is inhibited in an old mouse, so that the cognitive function is remarkably improved, and the senescence and related inflammatory indexes are inhibited. It is prompted that the FABP4 gene has the effect of promoting senescence and can serve as a molecular target for improving healthy senescence, and anew target and a new strategy are provided for senescence delaying.

The invention belongs to the technical field of mass spectrum detection, and discloses an in-vivo lipid three-dimensional mass spectrum imaging method based on an integral zebra fish model. The invention establishes the in-vivo lipid three-dimensional mass spectrum imaging method based on the integral zebra fish model, the method is applied to the research of a zebra fish C1 type Niemann-Pick disease model, and the result shows that fifteen lipids with obvious spatial distribution difference exist in different organs of zebra fish. According to the three-dimensional MALDI mass spectrum imagingmethod established by the invention, complex sample pretreatment is not needed, various compounds in a tissue sample can be detected at the same time, and the integrity of selected tissues and a multi-dimensional visual effect can be ensured, so that the spatial distribution difference between an experimental group and a control group can be visually and comprehensively obtained; the method has the advantages of high integrity and high chemical specificity.

The invention belongs to the technical field of molecular biology, and particularly relates to a comet assay method and device for DNA (deoxyribonucleic acid) damage of sugarcane lamins.According to the method, a large number of complete sugarcane lamina cell nucleuses can be obtained, so that the statistical magnitude required by the comet assay can be guaranteed; in order to obtain a good comet assay result, the premise is to obtain a large number of plant cell nucleuses, and the larger the statistical number in comet assay result analysis is, the more reliable the result is generally; the number of the cell nucleuses prepared in the steps S1 to S5 can reach 106/ml, and the experimental requirements are fully met; meanwhile, the change of the DNA damage of the sugarcane leaves can be well reflected by the experimental conditions established by the method disclosed by the invention; by comparing the experimental group and the control group, the change of the comet shape can be intuitively observed, and the detection sensitivity is very high, so that the method is favorable for being popularized to the change rule of DNA damage in numerous life activities such as apoptosis of sugarcane leaves, adversity stress response and diseases.