36 results about "Treatment and control groups" patented technology

The invention discloses an EST protease activity detection method which comprises the following steps: a, setting an EST experimental group, a negative control group and a blank control group; b, carrying out incubation reaction on the EST experimental group, the negative control group and the blank control group to obtain respective reaction mixtures; c, detecting the influence of the reaction mixture on breast cancer cell proliferation to obtain the proliferation rate of each group; and d, calculating the enzymatic activity of the EST protein. The method disclosed by the invention is simple and easy to implement, good in repeatability and capable of effectively detecting the activity of the EST protease.

The application discloses application of a CCL15 neutralizing antibody in preparation of a medicine for inhibiting growth of liver cancer, and comprises the following steps: S1, constructing a mouse liver cancer PDX model and performing passage; S2, randomly dividing the immunodeficient mouse after passage into a control group and an experimental group, and performing dosing treatment on the control group and the experimental group every three days; S3, PDX model tumor tissue evaluation, stripping tumor tissues after 11 times of dosing of the control group and the experimental group, and respectively observing and recording the volume and weight of the tumor tissues of the control group and the experimental group; the method for inhibiting growth of liver cancer provided by the application, CCL15 acts on the stellate cells to activate the stellate cells and make the expression spectrum of the stellate cells change significantly, the CCL15 neutralizing antibody has an anti-tumor effect in the liver cancer PDX model through injection, and tumor-derived CCL15 can indirectly promote growth of liver cancer cells by activating the hepatic stellate cells.

The embodiment of the invention provides an abnormal state attribution method and device, electronic equipment and a storage medium, and the method comprises the steps: obtaining abnormal feedback data, the abnormal feedback data being used for indicating a target user, and the target user being a user reporting an abnormal operation state of a client; according to the abnormal feedback data, first experimental group data are obtained, and the first experimental group data represent experimental groups of at least two function contrast experiments hitting the target user; according to the first experimental group data, a target function comparison experiment is obtained, the target function comparison experiment is determined based on the feedback permeability of the experimental group corresponding to the first experimental group data, and the feedback permeability represents the proportion of target users in the experimental group. Through the abnormal feedback data, the experiment group of the comparison function experiment hitting the target user is determined, and then the target function comparison experiment causing the abnormal operation state of the client is determined from the experiment group, so that the attribution from the abnormal operation state to the function comparison experiment is realized.

Sample delta monitoring is described herein. In one or more implementations, first and second experimental groups are defined in a randomized controlled trial. A first set of data indicative of a first experimental group in the randomized controlled trial is received. A second set of data indicative of a second experimental group in the randomized controlled trial is received. Based on sample counts of the first and second sets of data, an indication is generated that the experimental groups in the randomized controlled trial are skewed.

A method for analytically detecting and determining the transfection efficiency of fluorinated lipid nanoparticles (FLNPs) by utilizing multi-nuclear magnetic resonance comprises the steps of (1) performing 19F magnetic resonance spectroscopy (19F MRS) on the FLNPs to obtain a fluorine spectrum of a control group; (2) transfecting the FLNPs into cells or living mice and performing 19F MRS to obtain a fluorine spectrum of an experimental group; and (3) predicting FLNPs transfection efficiency by calculating the difference in target fluorine peak areas between the experimental and control groups. The method demonstrates excellent specificity, reproducibility, stability, and linearity, providing a robust technical foundation for large-scale screening of FLNPs-based nanomedicines.

The application discloses an analysis method, device and equipment of core molecules in the aging process, and a medium, and relates to the technical field of aging analysis. The method comprises the following steps: obtaining original data, dividing the original data into a control group and a plurality of experimental groups, and preprocessing the original data; performing aging mode clustering on the preprocessed original data to obtain a clustering result; performing duplicate removal GO enrichment analysis on each clustering mode, and visualizing the analysis result; taking the union of the molecular data of each experimental group after missing value screening and the molecular data of the control group after missing value screening, and analyzing the molecular data in the union through a differential analysis method to obtain a differential analysis result; performing correlation analysis on each clustering mode to obtain a correlation analysis result; and determining core molecules related to aging according to the correlation analysis result and the differential analysis result. The method can improve the accuracy of aging analysis.

The invention discloses a down-regulated CDC20-based breast cancer cell ferroptosis sensitivity detection method, and belongs to the technical field of detection methods, and the method comprises the following steps: cell grouping and transfection: culturing a breast cancer cell system, and setting an experimental group and a transfection negative control group; wherein in the experimental group, CDC20-siRNA transfection is carried out on breast cancer cells; an experimental group with the CDC20 relative expression quantity lower than 0.5 is screened through Western blot to serve as an optimal experimental group, and subsequent experiments are conducted; and then ferroptosis induction and ferroptosis induction and inhibition are carried out, and related detection is carried out. According to the invention, CDC20 is used as a regulation target of breast cancer ferroptosis sensitivity for the first time, and the target limitation of a traditional method is broken through; and the reliability and repeatability of a detection result are ensured through a standardized operation process and closed-loop verification logic.

The present application relates to the application of long-chain non-coding RNA HILAR inhibitor in the preparation of drugs for preventing or / and treating lung adenocarcinoma metastasis. The present application provides specific intervention means for HILAR, and verifies the effectiveness of inhibiting lung adenocarcinoma metastasis: after the expression of HILAR is targeted and silenced in vitro by small interfering RNA technology, the invasion ability of lung adenocarcinoma cells is significantly inhibited, and the metastasis phenotype is obviously reversed; more importantly, through the delivery of short hairpin RNA by adeno-associated virus vector, the formation and development of lung metastasis are effectively reduced in a lung adenocarcinoma metastasis mouse model, and the in vivo imaging shows that the tumor luminescence signal intensity of the experimental group is continuously lower than that of the control group, and the overall survival condition of the animal is improved.

The invention discloses application of bitter gourd exosomes in regulating intestinal flora. The method comprises the following steps: establishing an Alzheimer's disease mouse model, injecting the extracted balsam pear exosome into the mouse body in a gavage manner to serve as an experimental group drug, taking normal saline as a model negative control group, detecting the change of the intestinal flora of the AD mouse by applying a 16S amplicon sequencing principle, and detecting the change of the intestinal flora metabolite of the AD mouse by applying an LS-MS technology. An open field experiment, a nesting experiment and a water maze experiment are adopted to verify the treatment effect of the balsam pear exosome on AD mouse intestinal flora and metabolic level changes thereof. Results prove that the bitter gourd exosome extracted by the invention can effectively improve the intestinal flora micro-ecology of AD mice; the compound can be used for preparing a microecological preparation for regulating intestinal flora of neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis, different types of spinal cerebellar ataxia and the like and diseases such as cerebral apoplexy, cerebral injury, epilepsy, tumor and the like.

The present application relates to the field of radioactive toxicity detection, and more particularly to a method for evaluating low-activity radioactive 137 Cs toxicity based on marine Oryzias latipes, which comprises the following steps: step 1, setting the breeding conditions of Oryzias latipes, starting the exposure experiment in the adjusted exposure parameters of the radioactive 137 Cs; step 2, observing the reproductive state parameters of Oryzias latipes after the exposure experiment; step 3, dissecting the Oryzias latipes to obtain gonadal tissue; step 4, analyzing and comparing the significant level differences of each index of the control group and the experimental group by using single factor variance statistics of the data information in steps 2 and 3, and determining the toxicity level of the radioactive 137 Cs by the index differences. The present application realizes rapid evaluation of low-activity radioactive 137 Cs toxicity in seawater, and has the characteristics of simple processing process, high operation repeatability and high sensitivity.

The invention discloses an in-vitro biological efficacy evaluation method suitable for evaluating the efficacy of mesenchymal stem cells for treating systemic lupus erythematosus, and relates to the technical field of mesenchymal stem cell detection. The in-vitro biological efficacy evaluation method suitable for evaluating the efficacy of the mesenchymal stem cells for treating systemic lupus erythematosus comprises the following steps: S1, large-scale production of the mesenchymal stem cells; s2, sorting the B cells; s3, activating the cells B; s4, co-culturing the mesenchymal stem cells and the activated B cells, and taking a co-culture group as an experimental group; s5, detecting, analyzing and evaluating: detecting the regulation of the mesenchymal stem cells on the B cells, and analyzing and evaluating statistical data; b cell proliferation is stimulated through in-vitro sorting, the morbidity state of systemic lupus erythematosus (SLE) is simulated, in-vitro biological efficacy is detected through co-culture with mesenchymal stem cells, the method is rapid and simple, and the curative effect of mesenchymal stem cells of different sources can be evaluated.

The invention discloses a machine learning-based tumor early screening expiration VOCs marker identification and verification method. The method comprises the steps of obtaining and processing high-dimensional full-spectrum expiration VOCs data; a generalized linear model is adopted to evaluate the influence of the queue correlation difference on the expiratory VOCs data, and VOCs with significant interaction are removed; screening potential expired VOCs biomarkers with significant difference between an experimental group and a control group by adopting a difference volcano plot method in which a kernel weighting function is introduced; a Boruta feature screening algorithm based on a random forest is adopted, and an expiration VOCs biomarker panel most related to the tumor is constructed; and evaluating the tumor diagnosis and grading performance of the expired VOCs biomarker panel by adopting a machine learning algorithm based on multiple underlying logics. According to the method, a marker panel construction process based on a machine learning algorithm is created, and a biomarker panel suitable for various algorithms is accurately and efficiently captured from massive expired VOCs.

The invention provides an inhalation toxicology experiment system and experiment method for animals, and aims to evaluate the influence of drugs on the animals. According to the system, an integrated head-mounted bandage is fixed to the face of an animal, so that a biological electrode is in contact with the skin of the face of the animal, and electromyographic signals are collected. And the signal is wirelessly transmitted to the monitoring terminal through the control box, so that remote monitoring is realized. In an experiment, medicine is added into the aerosol generator and converted into fine particles, the fine particles are sent into the exposure box through the air supply device, and animals are exposed in the environment for a certain time. During the period, the system continuously monitors the aerosol concentration and the environmental oxygen content to ensure stable experimental conditions. And the toxic effect of the medicine is analyzed by comparing the change of the electromyographic signals of the control group and the experimental group. An advanced signal processing technology, such as band-pass filtering, independent component analysis and the like, is adopted to extract features from the electromyographic signals, and a classifier is trained by using a machine learning method to distinguish normal electromyographic signals from electromyographic signals influenced by drugs, so that the accuracy and reliability of experimental results are improved.

A device and method are taught for stimulating sympathetic nerves toward mitigating negative impacts to hematopoiesis. An autonomic nerve actuator and stimulator system with a graphic user interface (GUI), controller circuit which converts treatment parameters into a series of Building Block Waveforms (BBW) which are output to Current / Voltage Driver Circuitry (CDC) whose electrical stimulus (ES) outputs are coupled to one or more electrodes and / or electrode arrays positioned proximal specific nerve situations. The use of this in combination with conventional chemotherapy treatments, such as use of carboplatin, was found to significantly enhance hematopoiesis, with improvements in white and red blood cell counts, platelet concentration and hemoglobin concentration, in addition to other measurable characteristics which increased survival rates in the experimental groups tested.

This invention relates to the field of biomedical technology, and in particular to the application of hD1R protein in the preparation of drugs for the treatment of AML. Addressing the current lack of systematic and in-depth research on whether hD1R protein can directly act on AML cells and whether it has a synergistic effect with existing standard chemotherapy drugs (such as anthracyclines), this invention, through a series of in vivo and in vitro experiments and the construction of a NOD / SCID mouse transplantation model, confirms that hD1R protein alone can effectively inhibit AML cell proliferation, induce apoptosis, inhibit AML cell colony formation, and downregulate the expression of the key anti-apoptotic gene Bcl2 in AML cells. It also confirms that hD1R treatment can significantly prolong the survival of model mice. Furthermore, by setting up a Dox+hD1R group (combined experimental group), it is confirmed that when hD1R protein is used in combination with anthracycline chemotherapy drugs, it exhibits significant synergistic effects in inhibiting AML cell proliferation, promoting AML cell apoptosis, downregulating the expression of the anti-apoptotic gene Bcl2 in AML cells, and inhibiting AML cell colony formation.

The invention relates to the technical field of radioactive injury effect research, in particular to a radioactive eye injury model establishment method which comprises the following specific steps: S1, setting a plurality of experimental groups; the experimental group at least comprises a model group receiving different doses of ionizing radiation irradiation and a control group not receiving effective dose of radiation irradiation; s2, selecting an experimental animal, shaving and cleaning the target eye area of the animal needing to be irradiated, and anesthetizing the animal; s3, generating ionizing radiation of a preset type and energy by using a radioactive source, and performing local irradiation on the animal eye target area according to the preset total irradiation dose of each model group; s4, after irradiation is completed, the animals are nursed; the reaction of the animal after irradiation is observed, and scoring is carried out. The effectiveness of the established radioactive eye injury model can simulate typical expressions of eye injury under radiation irradiation of different doses, and a reliable animal model is provided for follow-up research of the generation mechanism of radioactive eye injury and evaluation of prevention and treatment measures.

The application discloses a positioning method and device for a target group and a medium, and is suitable for the technical field of data processing. By calling A / B experiments of an experimental group model and a control group model, the experimental group model and the control group model are respectively based on target function triggering users and target function non-triggering users to determine corresponding experimental group probabilities and control group probabilities, and then according to the experimental group probabilities and the control group probabilities, probability values corresponding to respective current users are determined, and current users corresponding to probability values meeting preset conditions are selected from the probability values to realize positioning of the target group. The experimental group model and the control group model based on the division of target function triggering and non-triggering can clearly determine that the positioning of the current target group is based on the target function, the target group positioned based on the target function is more accurate, and resource allocation is performed on the target group determined based on the target function to save resources.

The application provides a code reading large model parameter optimization method based on vllm deployment, which is implemented by a computer, first, the core viewpoints of a paper are combined, an experimental group is designed, a benchmark experimental group and an experimental control group are designed, experimental record data contents are used, experimental results are obtained, and mathematical analysis is combined to analyze the obtained data, then, suspected problem data is investigated and surveyed to obtain the best parameter configuration of the code reading large model relying on the vllm deployment, the core viewpoints of the paper can be combined to design reasonable benchmark experimental groups and experimental control groups, experimental record data contents are used, and mathematical analysis is combined to obtain the best parameter practice of the code reading large model relying on the vllm deployment. The application can ensure that the parameter optimization result of the code reading large model deployed on the vllm has a complete evidence chain, and has enough confidence to apply the optimized parameters in a real production environment.

The invention relates to the field of biomedicine, and particularly discloses an extracellular vesicle, a preparation method, a medicine and application. Comprising the following steps: obtaining and identifying human gall bladder epithelial stem cells (hGBECs), extracting hGBECs extracellular vesicles by using an ultracentrifugation method, establishing a CCL4-induced liver fibrosis mouse model, and performing experimental analysis. According to the research of the extracellular vesicles in the treatment of liver fibrosis, an experimental group and a plurality of groups of control groups are set; through data analysis, umbilical cord mesenchymal stem cell exosomes (MSC-EVs), human gallbladder epithelium stem cell extracellular vesicles (hGBECs-EV) and the pathological degree caused by liver fibrosis induced by CCL4 are obtained, collagen deposition and hepatic stellate cell activation caused by liver fibrosis induced by CCL4 are relieved, and a new method is provided for liver fibrosis treatment.

The invention relates to a method for evaluating ferroptosis sensitivity of NF2-related vestibular nerve sheath tumor Schwann cells. The method comprises the following steps: constructing NF2-mutated immortalized Schwann cells; carrying out ferroptosis induction and intervention experiments, and establishing a plurality of experiment groups; detecting ROS level, Fe < 2 + > concentration, GSH content, MDA level and protein expression of GPX4 and the like of each group of cells; sample preparation is carried out through a TEM method, and mitochondrial volume and morphological characteristics of each group of cells in a ferroptosis state are observed; detecting the cell viability, cell membrane damage and apoptosis proportion of each group of cells; and evaluating the influence of NF2 deletion on ferroptosis sensitivity. According to the method, a ferroptosis cell modeling scheme in which Erastin (an inducer) and Fer-1 (an inhibitor) are jointly intervened is designed, and a multi-dimensional ferroptosis evaluation system is combined, so that the stability and the result reproducibility of NF2 related vestibular nerve sheath tumor Schwann cell ferroptosis state evaluation are remarkably improved.

The invention relates to the technical field of plastic brain toxicity detection, and discloses a plastic brain toxicity detection method, which comprises the following steps: giving a preset number of food particles and water to mice in a target environment, and randomly dividing the mice into groups; after the mice are weighed, MNP with a preset concentration is administered to the experimental group regularly every day, normal saline is administered to the control group, 3D behavioral recording is conducted on the mice after several days of continuous administration, and behavioral analysis is conducted according to the recording result; and after behavioral analysis, performing injection anesthesia on the mice in each group, sampling brain tissues of the mice in each group, and performing brain cell analysis based on the brain tissues of the mice so as to detect the plastic brain toxicity. The toxicity of micro-plastic or nano-plastic can be efficiently and accurately detected through systematic experiment steps.

The invention discloses a construction method of a mouse autism intervention model, and belongs to the technical field of neurobiology and animal model construction. Comprising the following steps: S1, selecting a pregnant C57BL / 6J female mouse, performing VPA induction on the 12th to 13th embryo to form an experimental group, and injecting normal saline into another mouse to form a control group; s2, performing AAV-shRNA targeted mGluR5 virus injection on the mice in the experimental group, and retaining the injection for 15 minutes after the injection so as to avoid backflow; s3, recovering the postoperative mouse for 3 weeks, performing behavioral detection on the mouse at the age of 7 weeks, and performing protein and morphological analysis on the hippocampal tissue after an experiment; s4, carrying out behavioral experiments to evaluate ASD sample behavioral characteristics, detecting mGluR5 and PSD95 protein expression levels in the hippocampus tissues by using Western blot, and analyzing changes of synaptic structures by adopting Golgi staining. The VPA model provided by the invention can be used for wider autism research, and a model platform which is low in toxicity, easy to operate and repeatable is provided.

The utility model relates to the technical field of animal disease prevention and control biological experiment tools, and discloses a buckle type extensible agglutination experiment flat plate which comprises an anti-overflow groove, an anti-overflow opening and male and female buckle devices on the two sides of the agglutination experiment flat plate. The bottom of the anti-overflow groove is of a concave cambered surface structure, the upper end of the anti-overflow groove is connected with an anti-overflow opening, the upper end of the anti-overflow opening is flush with the upper surface of the agglutination experiment flat plate, and a certain distance is reserved between the upper end of the anti-overflow groove and the upper end of the anti-overflow opening. The buckle devices on the two sides of the agglutination experiment flat plate are male and female buckle devices, each formula buckle device is of a cuboid structure protruding outwards, a limiting and fixing device is arranged at the middle end of each formula buckle device, and each limiting and fixing device is of an arc fan-shaped structure protruding towards the periphery of the two sides. The female buckle device is of an inward-concave cuboid groove type structure, a limiting and fixing device is arranged at the middle end of the female buckle device, the limiting and fixing device is of an inward-concave hollow arc-fan-shaped structure, and the limiting and fixing device and the inward-concave hollow arc-fan-shaped structure, symmetrical to the limiting and fixing device, on the agglutination experiment flat plate body jointly form the female buckle limiting and fixing device. Hollow structures are formed among the formula buckle device, the female buckle device and the agglutination experiment flat plate main body, and the upper part of the formula buckle device and the lower part of the female buckle device are connected with the agglutination experiment flat plate and are flush with the upper surface and the lower surface of the agglutination experiment flat plate. According to the buckle type extensible agglutination experiment flat plate, through the cambered surface type structure at the bottom of the anti-overflow groove, a detector can conveniently mix all components required by an agglutination experiment; the anti-overflow opening connected with the anti-overflow groove can effectively prevent experimental components in the reaction hole from overflowing out of the hole or polluting components in other grooves when the agglutination experiment flat plate is shaken to mix the components or the agglutination experiment result is observed. Through sliding splicing and combination of the limiting fixing devices of the male and female type buckle devices on the two sides of the flat plate, the single agglutination experiment flat plate can be quickly expanded and connected to form a large plate, so that the number of detection samples on the agglutination experiment single plate can be multiplied, and work such as comparison of different agglutination results and shooting of image data can be efficiently carried out; and the agglutination experiment flat plate is made of a novel transparent, wear-resistant and reusable material, so that resource waste is avoided.

The invention discloses a BBB repairing agent evaluation method and system based on an ischemia reperfusion injury model, and relates to the technical field of biologication.The method comprises the following specific steps that the ischemia reperfusion injury model is built, and a sample is collected, specifically, an animal ischemia reperfusion injury model is built, and experimental animals are divided into an experimental group and a control group; brain tissue and blood samples of the two groups of animals are collected at a plurality of preset time nodes, and behavioral evaluation is carried out at the same time to obtain score data; according to the method, the deep learning model fused with the dynamic attention mechanism is constructed, the weights of different time nodes and different modal data on BBB repair effect evaluation after ischemia reperfusion injury can be automatically identified, the problems that early signals are ignored and key time node evaluation is deviated due to fixed static data weights in a traditional evaluation method are solved, and the accuracy of BBB repair effect evaluation is improved. The model can flexibly adjust the weight of each modal data according to the dynamic change characteristics of the BBB repair process, and a more reliable basis is provided for research, development and application of a repair agent.

The invention relates to the field of biomedicine, in particular to a nerve-organoid co-culture chip and a use method thereof, which can realize co-culture of nerves and cells / organoids under the condition of separation of culture media of the nerves and the cells / organoids, simulate signals of the nerves to a local microenvironment on a two-dimensional / three-dimensional level, and realize co-culture of the nerves and the cells / organoids. Interactions between nerves and various cells are dynamically observed in real time; a control group and an experiment group can be simultaneously arranged on one chip, and the difference and trend of the two groups can be observed in real time.

This invention belongs to the field of biotechnology, specifically relating to a polypeptide that improves stress response symptoms in animals and its preparation method. This invention prepares a new polypeptide and verifies that the polypeptide can improve the quality of nighttime sleep and daytime microsleep in cats after transport stress to a certain extent, alleviating the significant reduction in activity caused by transport stress. The experimental group showed significantly lower CSS scores than the control group within 1-3 days after transport, and the experimental group showed significant improvement in hiding behavior and escape intent, reducing the duration of stationary behavior and pacing time. The polypeptide can alleviate the effects of unfamiliar environments on cats; it can reduce the level of HPA axis hormones, thereby exerting an anti-stress effect, increasing the activity of GSH-Px, SOD, and CAT in cats, and reducing MDA levels. Furthermore, it can reduce the decrease in T-AOC levels and SOD activity in cats after transport stress, and reduce MDA levels in unfamiliar environments, indicating that the polypeptide can reduce the level of oxidative stress in cats during transport stress.

The invention belongs to the technical field of biology, and particularly relates to polypeptide for improving animal stress reaction symptoms and a preparation method of the polypeptide, new polypeptide is prepared, and it is verified that the polypeptide can improve night sleep and daytime micro sleep quality of cats after transportation stress to a certain extent and relieve great reduction of activity caused by transportation stress; the CSS score of the experimental group within 1-3 days after transportation is obviously lower than that of the blank group, the hiding behavior and the escape intention of the experimental group are obviously improved, and the trend of static behavior duration and pacing time can be reduced; the polypeptide can relieve the influence of an unfamiliar environment on cats; the polypeptide can reduce the level of HPA axis hormone so as to play an anti-stress role, improve the activity of GSH-Px, SOD and CAT of cats, reduce the MDA level, reduce the decrease of T-AOC level and SOD activity of cats after transport stress, and reduce the MDA level in an unfamiliar environment, which indicates that the polypeptide can reduce the oxidative stress level of cat bodies in the process of transport stress.

The invention discloses a detection method for killing efficiency of NK cells and a kit. The detection method comprises the following steps: S1, preparing a target cell suspension and an NK cell suspension; s2, setting experiment groups including a standard curve group, an experiment group and a blank group; the cells are inoculated to two 96-well plates respectively, each 96-well plate is provided with three multiple holes, and inoculation is carried out according to experimental groups; s3, respectively adding a CCK-8 reagent into the experiment holes of a 96-well plate, and measuring the absorbance of each hole at the position of 450 nm through a microplate reader; s4, placing another 96-well plate in the incubator for continuous co-culture for a certain period of time, respectively adding a CCK-8 reagent into each experiment hole, and measuring the absorbance of each hole at the position of 450 nm through the microplate reader; and S5, carrying out regression analysis on the OD value and the cell number of the standard curve group, and if R2 is greater than or equal to 0.9, calculating the killing rate according to a formula. According to the detection method and the kit for the killing efficiency of the NK cells, the killing activity of the NK cells on K562 and A549 is detected by applying CCK-8, the detection method for detecting the killing tumor cells of the NK cells through a CCK-8 method is established, operation is simpler and more convenient, consumed time is short, repeatability is good, damage to the cells is small, and the result is accurate.

The invention belongs to the field of anti-tumor drugs, and particularly relates to an alkane ligand modified heptanuclear high-nuclear cluster compound as well as a preparation method and application thereof. The molecular formula of the heptanuclear high-nuclear cluster compound modified by the alkane ligand is [Na4 (H2O) 4 [{Na3Ce2W (H2O) 9} {Ce (C11H26N2O6)}] 2 {SbW8O30} 2}]. 4H2O, and the molecular weight of the heptanuclear high-nuclear cluster compound modified by the alkane ligand is 6616.36. When the compound provided by the invention is applied to an MG-63 cell migration experiment, after 24 hours, the scratch healing rates of MG63 cells are respectively a control group (33.01 + / -0.69)% and an experimental group (15.28 + / -5.19)%. Compared with a control group, the scratch healing rate of an experimental group is remarkably reduced by about 53.7%, and the difference has statistical significance (Plt; 0.01). The result shows that the compound can effectively inhibit the migration ability of MG-63 cells.

The invention discloses an application of a GSDMD molecular inhibitor NU6300 in preparation of a medicine for treating heart transplantation rejection. The GSDMD molecular inhibitor NU6300 is applied to a constructed heart transplantation model, and after treatment of the GSDMD molecular inhibitor NU6300, it is observed that the survival time of heart grafts of an experimental group and a control group is prolonged, the GSDMD-mediated pyroptosis level is reduced, and the infiltration degree of inflammatory cytokines in the grafts is weakened. Therefore, the invention can provide a new therapeutic drug for treating patients after organ transplantation, and can be applied to organ transplantation treatment.