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Camellia oil lipidosome, preparation method thereof and application of camellia oil lipidosome to preparing acute liver injury treating drugs

A technology of oil liposome and camellia oil, applied in the directions of liposome delivery, drug combination, pharmaceutical formulation, etc., can solve problems such as unfavorable mass preparation, cumbersomeness, etc., achieves alleviation of necrosis and cell shrinkage, good repeatability, and easy preparation the effect obtained

Inactive Publication Date: 2019-06-21
HAINAN MEDICAL COLLEGE
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these substances are still in the verification stage, and the method of obtaining active ingredients is cumbersome, which is not conducive to large-scale preparation and cannot meet the large demand for drugs. Therefore, it is urgent to develop a drug for acute liver injury that is easy to obtain and has a significant effect.

Method used

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  • Camellia oil lipidosome, preparation method thereof and application of camellia oil lipidosome to preparing acute liver injury treating drugs
  • Camellia oil lipidosome, preparation method thereof and application of camellia oil lipidosome to preparing acute liver injury treating drugs
  • Camellia oil lipidosome, preparation method thereof and application of camellia oil lipidosome to preparing acute liver injury treating drugs

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preparation example Construction

[0019] The invention provides a kind of preparation method of camellia oil liposome, comprising the following steps:

[0020] Suspending 1 mL of camellia oil, 0.05-1.5 g of lecithin and 0.01-0.2 g of phosphatidylserine in 5-50 mL of deionized water, ultrasonication, oscillation and standing still to obtain camellia oil liposomes.

[0021] In the present invention, the suspension system is preferably 1mL camellia oil, 0.1-1.0g lecithin and 0.02-0.15g phosphatidylserine suspended in 10-40mL deionized water, more preferably 1mL camellia oil, 0.2g lecithin and 0.02g Phosphatidylserine was suspended in 10 mL of deionized water.

[0022] In the present invention, the power of the ultrasound is preferably 30-200W, more preferably 50-150W, more preferably 100W. The temperature of the ultrasound is preferably lower than 50°C, more preferably 10-40°C. The time of the ultrasound is preferably 10-20 min. In the present invention, there is no special limitation on the instrument used fo...

Embodiment 1

[0029] 1mL camellia oil (SCY), 0.2g lecithin, 0.02g phosphatidylserine, suspended in 10mL deionized water. The sonicator was used for 5 minutes, the ultrasonic power was 200W, the temperature of the mixture was 40°C during the sonication, and the mixture was vortexed for 5 minutes. Stand still for 5 minutes to obtain camellia oil liposomes. The morphology of liposomes was observed by light microscopy.

[0030] result figure 1 shown. In aqueous solution, the prepared camellia oil liposome (Lip-SCY) was in uniform white emulsion state ( figure 1 -A). Microscopic observation shows that liposomes are spherical or nearly spherical, with a diameter of 1-10 μm ( figure 1 -B).

Embodiment 2

[0032] 1mL camellia oil, 1.0g lecithin, 0.1g phosphatidylserine, suspended in 30mL deionized water. Ultrasonic breaker was used for 10 minutes, the power of ultrasound was 150W, the temperature of the mixture was 40°C during the ultrasound process, and the mixture was vortexed for 5 minutes. Stand still for 8 minutes to obtain camellia oil liposomes. The morphology of liposomes was observed by light microscopy.

[0033] In aqueous solution, the prepared camellia oil liposome (Lip-SCY) was in the state of uniform white emulsion. Microscopic observation showed that the liposome was spherical or nearly spherical, with a diameter of 1-10 μm.

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Abstract

The invention provides a camellia oil lipidosome, a preparation method of the camellia oil lipidosome and application of the camellia oil lipidosome to preparing acute liver injury treating drugs andbelongs to the technical field of liver injury. The camellia oil lipidosome is prepared by suspending 1 mL of camellia oil, 0.05-1.5 g of lecithin and 0.01-0.2 g of phosphatidylserine into 5-50 mL ofdeionized water and performing ultrasonic treatment, oscillation and standing. The prepared camellia oil lipidosome is a uniform white emulsion, and under microscopic examination, is in the shape of spherical or quasi-spherical bubbles with a diameter of 1-10 mu m. Detection of the liver function indexes of activity of alkaline phosphatase (ALP) and alanine aminotransferase (ALT) in the serum of mice of an experimental group and a control group and observation of the liver tissues of the mice show that the camellia oil lipidosome can achieve significant protecting effects on acute liver injuryand is higher in efficacy than camellia oil. Therefore, the camellia oil lipidosome can be applied to preparing the acute liver injury treating drugs.

Description

technical field [0001] The invention belongs to the technical field of liver injury, and in particular relates to a camellia oil liposome, a preparation method thereof and an application in preparation of a medicine for treating acute liver injury. Background technique [0002] At present, the prevention and treatment of liver injury is still a serious issue at home and abroad. Choosing an appropriate liver injury model is the prerequisite for doing a good job in the prevention and treatment of liver injury. It is of great practical significance to establish experimental liver injury animal models to study the mechanism of liver injury, screen hepatoprotective drugs, and explore the principle of hepatoprotective action. [0003] There are many ways to treat acute liver injury. At present, chemical drugs are commonly used in clinical treatment of acute liver injury. Although chemical drugs have significant curative effects, they are drug-dependent, and some patients may have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/82A61K9/127A61K47/24A61P1/16
Inventor 冯棋琴喻其林易聪张帆周静
Owner HAINAN MEDICAL COLLEGE
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