Application of p38 gamma in preparation of pancreatic cancer prognosis diagnostic reagent

A pancreatic cancer and reagent technology, applied in the field of diagnostic reagents for the prognosis of pancreatic cancer, can solve the problem that the relationship between the occurrence, development and prognosis of pancreatic cancer is not clear, and achieve the effect of promoting invasion and metastasis

Active Publication Date: 2020-05-29
SUN YAT SEN UNIV CANCER CENT
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Problems solved by technology

[0006]In existing studies, the relationship between p38γ and the

Method used

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  • Application of p38 gamma in preparation of pancreatic cancer prognosis diagnostic reagent
  • Application of p38 gamma in preparation of pancreatic cancer prognosis diagnostic reagent
  • Application of p38 gamma in preparation of pancreatic cancer prognosis diagnostic reagent

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Experimental program
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Embodiment Construction

[0034] The technical solution of the present invention will be further described below in combination with experiments.

[0035] Expression and Prognosis of p38γ in Cancer Tissues of Patients with Pancreatic Cancer

[0036] First, the inventors detected the expression of p38γ in tumor tissue of 49 PDAC patients, and found that the expression of p38γ in tumor tissue was significantly higher than that in normal tissue, and the expression level of p38γ gradually increased with the progression of pancreatic cancer. Subsequent inventors retrospectively analyzed the relationship between the expression of p38γ in 80 cases of pancreatic ductal adenocarcinoma tissue and disease progression and survival prognosis, and found that the overall survival of patients with high expression of p38γ was significantly lower than that of patients with low expression.

[0037] Detection of p38γ expression in paraffin tissue sections by immunohistochemistry

[0038] (1) Paraffin sections were baked ...

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Abstract

The invention discloses application of p38 gamma in preparation of a pancreatic cancer prognosis diagnostic reagent. The inventor finds that expression of p38 gamma in cancer tissues of a pancreatic cancer patient is remarkably higher than that of p38 gamma in normal tissues, the expression level of p38 gamma is gradually increased along with disease progression, and the high expression of p38 gamma is related to adverse prognosis of the patient. A pancreatic duct adenocarcinoma animal model K-RasG12D-p53R172H-Pdx-Cre (KPC) is constructed as a control group for research. A pancreatic ductal epithelial cell p38 gamma knockout pancreatic cancer animal model (KPC-p38 gamma-KO) is constructed as an experimental group by applying a Cre/LoxP technology. The p38 gamma knockout is found to be capable of remarkably reducing the size of a tumor and prolonging the lifetime of KPC mice. The median survival time of the mice in the KPC group is 160 days, most KPC-p38 gamma-KO mice still survive in the statistical time, and the median survival time is longer than 300 days. A further experimental result shows that high expression of p38 gamma can promote invasion and metastasis of a pancreatic cancer. Therefore, p38 gamma can well determine the risk and prognosis of the pancreatic cancer.

Description

technical field [0001] The invention relates to a diagnostic reagent for pancreatic cancer prognosis. Background technique [0002] Cancer is a serious threat to people's health. With the development of medicine, more and more cancers have had better treatment effects. Among many cancers, pancreatic cancer is known as the "king of cancers", and it is a cancer that has not yet been conquered. In the prior art, pancreatic cancer has unsatisfactory effects in terms of prevention, diagnosis, treatment and prognosis. According to the "2015 China Cancer Statistics" data, pancreatic cancer ranks 9th and 6th in the incidence and mortality of overall malignant tumors in my country, and it is showing a rapid upward trend. About 3 / 4 of pancreatic cancer patients die within 1 year after diagnosis, and the 5-year survival rate is only 6%, making it the veritable king of cancer. [0003] The pathogenesis of pancreatic cancer is unknown, and its natural course is about 20 years. During ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574
CPCC12Q1/6886G01N33/57438C12Q2600/118C12Q2600/156C12Q2600/158G01N2800/52G01N2333/912
Inventor 王芳符立梧
Owner SUN YAT SEN UNIV CANCER CENT
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