Method for evaluating efficacy of anti-tumor drug at cellular level

Abstract

The invention relates to a method for evaluating the efficacy of an anti-tumor drug at a cellular level. The method comprises the following steps of setting anti-tumor drug experiment groups and control groups with different concentrations, taking a group with the anti-tumor drug concentration of 0 and added with heavy water as a positive control group (pos), and taking a group with the anti-tumordrug concentration of 0 and not added with heavy water as a negative control group (neg); digesting the tumor cells cultured in the experimental group and the control group, centrifugally cleaning and dropwise adding to a low-Raman background chip for Raman detection, calculating the CD / (CD + CH) of the experimental group and the control group according to the obtained Raman spectra, and determining whether the anti-tumor drug to be detected is effective to the tumor cells or not according to the CD / (CD + CH) of the experimental group and the control group. Compared with the prior art, the method has the advantages that the effectiveness of the anti-tumor medicine is evaluated from the cell metabolism level, compared with a traditional method for evaluating the effectiveness of the medicine by calculating the number of cells, the anti-tumor medicine which only inhibits cell growth but does not inhibit cell metabolism can be evaluated more accurately, and then medication is guided.

Description

technical field [0001] The invention belongs to the technical field of drug screening, and in particular relates to a method for evaluating the efficacy of antitumor drugs at the cell level. Background technique [0002] In the in vitro research of anti-tumor drugs, the commonly used drug efficacy evaluation methods mainly include CCK8, MTT, SRB method, etc. These methods generally involve co-cultivating anti-tumor drugs and cells before testing, and the co-cultivation time is generally 48-72h , and then add the reaction reagent to continue culturing for several hours, and use the color reaction between the reagent and the cell components and the principle that the OD value of the solution after the reaction is linearly related to the number of cells to calculate the half effective concentration of the drug. However, these methods have complex operations, Disadvantages such as high price and unstable results. Contents of the invention [0003] The purpose of the present i...

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Problems solved by technology

[0002] In the in vitro research of anti-tumor drugs, the commonly used drug efficacy evaluation methods mainly include CCK8, MTT, SRB method, etc. These methods generally involve co-cultivating anti-tumor drugs and cells before testing, and the co-cultivation time is generally 48-72h , and then add the reaction reagent to continue culturing for several hours, and use the color reaction between the reagent and the cell components and the principle that the OD value of the solution after the reaction is linearly related to the number of cells to calculate the half effective concentration of the drug. However, these methods have complex operations, Disadvantages such as high price and unstable results

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