Method for researching effects of Dickkopf-1 and cell apoptosis in steroid-induced avascular necrosis of femoral head (SANFH)

A technology for ischemic necrosis and femoral head, which is applied in the field of medical research and can solve the problems of increasing the expression of Wnt antagonist Dickkopf-1, and not going deep into the apoptosis mechanism of osteocytes and osteoblasts.

Inactive Publication Date: 2015-11-11
刘万林
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Problems solved by technology

Unfortunately, these studies have not yet penetrated into the research on the mechanism of apoptosis of osteocytes and osteoblasts.
And some studies have shown that in other diseases such as liver cancer, gastric cancer, etc. Wnt signaling pathway is closely rel

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  • Method for researching effects of Dickkopf-1 and cell apoptosis in steroid-induced avascular necrosis of femoral head (SANFH)
  • Method for researching effects of Dickkopf-1 and cell apoptosis in steroid-induced avascular necrosis of femoral head (SANFH)
  • Method for researching effects of Dickkopf-1 and cell apoptosis in steroid-induced avascular necrosis of femoral head (SANFH)

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[0070] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0071] figure 1 Show the research method process flow of Dickkopf-1 of the present invention and apoptosis in steroid-induced avascular necrosis of the femoral head, as shown in the figure, the present invention is realized in this way, a kind of Dickkopf-1 and apoptosis The research method of the role in steroid-induced avascular necrosis of the femoral head is to collect the femoral head specimens of SANFH removed by total hip replacement as the experimental group, and the femoral head specimens after femoral neck fracture hip replacement as the control group. After fixation and decalcification...

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Abstract

The invention discloses a method for researching the effects of Dickkopf-1 and cell apoptosis in steroid-induced avascular necrosis of femoral head (SANFH). The method comprises the following steps: by taking the femoral head sample of SANFH taken through total hip replacement as an experiment group and taking the sample after the hip replacement for fracture of neck of femur as a contrast group, cutting the femoral head sample, fixing and decalcifying the cut femoral head sample to prepare slices, observing the morphologic change of osteocytes by an electronic microscope, observing the pathological change of the osteocytes by a light microscope, counting the vacant bone lacuna, detecting the expression of Dickkopf-1 by an immunohistochemical staining technique, thus exploring the effects of Dickkopf-1 and cell apoptosis in SANFH. The method can be used for providing assistance for early diagnosis and curing of SANFH and also providing a theoretical basis for clinical prevention and curing of SANFH in future.

Description

technical field [0001] The invention belongs to the field of medical research, and particularly relates to a research method for the effect of Dickkopf-1 and cell apoptosis in steroid-induced avascular necrosis of the femoral head. Background technique [0002] Steroid-induced Avascular Necrosis of Femoral Head (Steroid-induced Avascular Necrosis of Femoral Head, SANFH) refers to the pathological process caused by the death of active components of the femoral head due to long-term use or short-term heavy use of adrenal cortex hormones (hereinafter referred to as hormones). As early as the 1950s, it was discovered that a large number of hormones can lead to femoral head necrosis. At present, due to the wide application of glucocorticoids clinically, the number of clinical cases of SANFH has increased significantly. SANFH is the most common type of non-traumatic necrosis of the femoral head. SANFH mainly affects young and middle-aged people, mostly bilateral, and its necrosi...

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Application Information

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IPC IPC(8): G01N21/00G01N23/04G01N33/543
Inventor 刘万林赵振群白锐王文选王勇
Owner 刘万林
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