Metabolomic analysis method of safflower against scleroderma based on liquid chromatography-mass spectrometry

Abstract

The invention discloses a safflower scleroderma-resisting metabonomics analysis method based on liquid chromatography-tandem mass spectrometry. The safflower scleroderma-resisting metabonomics analysis method comprises the following steps: 1) dividing healthy mice into three experimental groups including a control group, a scleroderma model group and a safflower treatment group; subcutaneously injecting a bleomycin solution into the mice of the scleroderma model group and the safflower treatment group every day to replicate a mouse scleroderma model; feeding safflower water decoction into themice of the safflower treatment group every day and treating; modeling for 28 days and judging whether modeling succeeds or not by physiological changes and pathological changes; 2) after the modelingsucceeds, taking blood serum samples of the mice of the three experimental groups and detecting by adopting the liquid chromatography-tandem mass spectrometry to obtain a fingerprint spectrum of theblood serum samples of the mice of the three experimental groups; 3) analyzing the fingerprint spectrum by adopting multivariate statistics to obtain a differential metabolite; identifying to obtain abiomarker. According to the method disclosed by the invention, the biomarker of safflower scleroderma-resisting metabonomics is obtained and a safflower scleroderma-resisting multi-component and multi-target acting mechanism is analyzed and identified.

Description

Technical field [0001] The invention belongs to the research field of traditional Chinese medicine, and particularly relates to a metabolomics analysis method of safflower anti-scleroderma action mechanism based on liquid mass spectrometry. Background technique [0002] Traditional Chinese medicine is a treasure of the Chinese nation. It has played a role in escorting the healthy reproduction of the nation in the 5,000-year-old history of the Chinese nation. Traditional Chinese medicine has the characteristics of multiple components and multiple targets. From the overall system level of the human body Playing a role is the essence of Chinese medicine. However, due to the uncertainty of the effective components of Chinese medicine and the overall ambiguity of the mechanism of action, the modernization of Chinese medicine has now encountered a huge bottleneck. Different from the single-component form of western medicine, traditional Chinese medicine has many chemical components, a...

AI Technical Summary

Problems solved by technology

Theoretically, the mechanism of action of safflower against SSc should be "multiple pathways, multi-components, and multi-targets", but there is still a lack of effective experimental methods and technical means to verify the mechanism of action of safflower from a systemic perspective. Generation of metabolomics can provide strong support for elucidating its mechanism of action

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