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Treating method for viscous fluid specimen

A processing method and specimen processing technology, applied in the field of biochemistry, to achieve the effects of flexible application, reducing specimen viscosity, and facilitating specimen processing

Inactive Publication Date: 2016-04-20
北京澳利文生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of processing method of viscous liquid sample, thus solve the foregoing problems existing in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The method of processing the viscous liquid specimens into the treatment liquid for microbial culture: take 20 qualified clinical sputum specimens, and set up the control group and the experimental group for each, specifically: take the viscous liquid specimen Ⅰ with a volume of XmL and add it to In XmL pre-prepared treatment agent A, do repetitions as the experimental group; take XmL viscous liquid specimen I with a volume of XmL and add it to XmL normal saline, do repetitions, as the control group; Shake at 37.5°C for 0.8-1.2 hours to obtain a viscous liquid specimen treatment solution, and complete the treatment of the viscous liquid specimen.

[0022] 1L of the treatment agent A includes: 0.27g of potassium dihydrogen phosphate, 1.42g of disodium hydrogen phosphate, 8g of sodium chloride, 0.2g of potassium chloride, 5mL of Tween 20 and 25g of biological enzymes, the The pH of the treatment agent A is 6.5-7.5; the biological enzymes include: 10g of trypsin, 10g of ch...

Embodiment 2

[0026] The difference between this embodiment and Example 1 is that: 1L of the treatment agent A includes: 0.135g potassium dihydrogen phosphate, 0.71g disodium hydrogen phosphate, 4g sodium chloride, 0.1g potassium chloride, 2.5mL Tween 20 and 12.5g biological enzyme, the pH of the treatment agent A is 7.0.

Embodiment 3

[0028] The difference between this embodiment and Example 1 is that: 1L of the treatment agent A includes: 0.405g potassium dihydrogen phosphate, 2.13g disodium hydrogen phosphate, 12g sodium chloride, 0.3g potassium chloride, 7.5mL Tween 20 and 37.5g biological enzyme, the pH of the treatment agent A is 7.5.

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Abstract

The invention discloses a treating method for a viscous fluid specimen, and relates to the technical field of biochemistry. The method includes the steps that the viscous fluid specimen is added into pre-prepared treating agents with the volume equal to that of the viscous fluid specimen, and repetition is carried out to obtain experimental groups; the experimental groups are treated according to the detected type of the viscous fluid specimen to obtain viscous fluid specimen treating fluids, and then the viscous fluid specimen is treated. By means of the method, the liquefaction time of the specimen is significantly shortened, the viscosity of the specimen is reduced, and the diagnosis precision is not affected; the method can be flexibly applied according to diagnosis purposes; meanwhile, medical cost is not increased.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a method for processing viscous liquid specimens. Background technique [0002] In clinical examination, viscous specimens such as thick sputum, pus, pleural effusion, gastric juice, tracheal fluid, pharyngeal mucus, and guiding urethral secretion are often encountered. These specimens generally require initial treatment before microbial culture, biochemical examination, and immune examination. However, viscous specimens are not easy to smear or smear unevenly, which may easily lead to missing the cultivation of pathogenic microorganisms. With the development of biochemical diagnostic technology, immunodiagnostic technology and molecular diagnostic technology with PCR technology as the main method, it has gradually become an indispensable method in clinical diagnosis. It is an indispensable means and has higher requirements for the processing of viscous specimens. Pollutant...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04G01N1/28
CPCC12Q1/6806C12Q1/04G01N1/28
Inventor 朱克先马国富陈政刘剑峰乔建秀
Owner 北京澳利文生物科技有限公司
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