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Molecular marker related to senescence and health senescence and application of molecular marker in improving health senescence

A molecular marker and aging technology, which is applied in improving healthy aging. In the field of molecular markers, it can solve the problems of insufficient detection depth, lack of repeated verification, and inability to accurately quantify low-abundance proteins, so as to achieve the effect of accelerating aging.

Active Publication Date: 2020-11-13
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these studies generally have insufficient detection depth, cannot accurately quantify low-abundance proteins, and are difficult to reflect the broad-spectrum regulation of protein factors in blood circulation during the aging process, and there is a lack of repeated verification between the findings of various studies

Method used

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  • Molecular marker related to senescence and health senescence and application of molecular marker in improving health senescence
  • Molecular marker related to senescence and health senescence and application of molecular marker in improving health senescence
  • Molecular marker related to senescence and health senescence and application of molecular marker in improving health senescence

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 The improvement effect of Taijiquan exercise on various indexes of human body

[0042] Enrolled persons: A young control group aged 22-25, an elderly control group aged 60-69, and a group aged 60-69 who have practiced Taijiquan for more than 3 years were included as the youth control group, the elderly control group and the Taijiquan group.

[0043] 1. Cerebrovascular function test

[0044] Cerebrovascular function detector (GT-3000) was used to analyze bilateral common carotid artery hemodynamics of the subjects, and Doppler probe was used to collect cerebral blood flow information to obtain cerebral blood flow velocity and average blood flow; The pressure sensing probe collects information related to the elasticity of cerebral blood vessels, and obtains relevant indicators such as peripheral resistance and pulse wave velocity. Detection indicators: average flow (Qmean), average flow velocity (Vmean), maximum flow velocity (Vmax), minimum flow velocity (V...

Embodiment 2

[0055] Example 2 Proteomics screening

[0056] The plasma samples of the youth control group, the elderly control group, and the Tai Chi group were randomly divided into three mixed samples according to gender, each mixed sample contained 15 individual samples, and the 10-standard, 36-level tandem mass spectrometry label (Tandem Mass Tag,TMT) protein profile identification, screening protein molecular markers whose abundance changes during aging and whose change trend is reversed in the Tai Chi group.

[0057] The result is as figure 1 Shown: A. ELISA detection of IGF-1 expression in serum. B. ELISA detection of FABP4 expression in serum. Protein profiling reveals Tai Chi exercise reverses circulating FABP4 elevations in older adults.

Embodiment 3

[0058] Example 3 Construction of FABP4 transgenic mice

[0059] Establishment of natural aging mouse model: purchase 8-week-old C57BL / 6 wild-type male mice, and inject them with adenovirus AAV9-shNeg and adenovirus AAV9-shFabp4 respectively to construct Fabp4 knockout mice and its control mice. Mice were cultivated to 20 months old to establish a natural aging mouse model. When the mice were 20 months old, relevant indicators were detected.

[0060] Experimental grouping: 20 aged mice were transfected with adenovirus AAV9-shNeg at 20 months, 20 aged mice were transfected with adenovirus AAV9-shFabp4 at 20 months, and 20 young 8-week-old mice were wild-type C57BL / 6.

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a molecular marker related to senescence and health senescence and application of the molecular marker in improvinghealth senescence. According to the invention, Tai Chi exercisers are used as an experimental group, protein spectra are compared with those of normal young people and old people, the FABP4 protein is screened out, the expression of the FABP4 protein in blood of old people is significantly increased compared with that of young people, but the trend of the FABP4 protein in Tai Chi exercises of oldpeople is significantly reversed. The FABP4 gene expression is knocked down or the FABP4 activity is inhibited in an old mouse, so that the cognitive function is remarkably improved, and the senescence and related inflammatory indexes are inhibited. It is prompted that the FABP4 gene has the effect of promoting senescence and can serve as a molecular target for improving healthy senescence, and anew target and a new strategy are provided for senescence delaying.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular marker related to aging and healthy aging and its application in improving healthy aging. Background technique [0002] The world is ushering in the era of "global aging", while my country has entered an aging society and is in the stage of deepening aging. It is estimated that by 2020, the population of the elderly over 60 years old will increase to about 255 million people, accounting for about 17.8% of the total population, and the social burden brought by aging is increasing day by day. Aging is closely related to numerous age-related diseases, including Alzheimer's disease, Parkinson's disease, stroke, peripheral neuropathy, macular degeneration, cataracts, presbycusis, type II diabetes, osteoporosis, osteoarthritis, arterial Atherosclerosis, benign prostatic hyperplasia and various cancers, etc. [0003] Aging is a complex process of continuous decline...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883A61K45/00A61P39/06A61P25/00A61P1/16A61P29/00
CPCC12Q1/6883A61K45/00A61P39/06A61P25/00A61P1/16A61P29/00C12Q2600/158
Inventor 王志华汤立许李丽莉胡依萌
Owner WUHAN UNIV
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