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Method for screening cotton drought-resistant related genes

A cotton and gene technology, applied in the field of screening cotton drought resistance-related genes

Active Publication Date: 2017-06-27
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on the changes of DNA methylation induced by drought stress in upland cotton

Method used

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  • Method for screening cotton drought-resistant related genes
  • Method for screening cotton drought-resistant related genes
  • Method for screening cotton drought-resistant related genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1. Using Methylation Sensitive Amplified Polymorphism (MSAP) to Screen Upland Cotton Drought Resistance Related Genes

[0051] 1. Test materials

[0052] The present invention adopts H177 among the cotton drought-resistant materials, adopts the sand culture method, and grows seedlings in an incubator (light for 14 hours, 30° C.; dark for 10 hours, 25° C.). Select seeds with plump and uniform seeds, disinfect them with 5% sodium hypochlorite, rinse them with sterile water, germinate them in a sterile petri dish for 36 hours, and select seeds with consistent germination for planting. Drought stress was carried out when the cotton seedlings grew to three leaves and one heart, and the water content of sandy soil was monitored. When the relative soil water content dropped to about 7.0%, leaf samples were taken, quick-frozen in liquid nitrogen, and stored at -80°C. Among them, the morphological changes of H177 before and after drought stress in cotton are as follows:...

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Abstract

The invention discloses a method for screening cotton drought-resistant related genes. The method comprises the steps that 1, an experimental group for conducting drought stress treatment on cotton to be detected and a control group for conducting no drought stress treatment on the cotton to be detected are arranged; 2, the methylation levels and / or modes of genomes DNA of the cotton to be detected in the experimental group and the control group are detected and compared, and genes different in methylation level and / or mode of genome are found in the two groups and are the cotton drought-resistant related genes or serve as cotton drought-resistant related gene candidates. The methylation level and / or mode change differences of upland cotton seedlings under drought stress are analyzed by utilizing an MSAP technology, the methylation levels and / or mode changes of CCGG locus cytosine before and after stress are analyzed, the drought-resistant related genes are searched by utilizing methylated difference fragments, and a basis can be provided for cotton drought-resistant epigenetic regulation mechanism research and drought-resistant related gene screening.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for screening genes related to drought resistance in cotton, in particular to a method for screening genes related to drought resistance in cotton by detecting changes in methylation levels of cotton genomes treated with and without drought stress. Background technique [0002] Global climate change is intensifying, water resources are scarce, the greenhouse effect is intensifying, and soil salinization is serious, which has seriously restricted my country's agricultural production. From a global perspective, drought affects the most extensive area and causes the most serious economic losses. It is considered to be one of the most serious natural disasters in the world. 28% of the world's land is arid, and 2 / 3 of our country is arid. With the increasingly serious conflicts in my country's grain and cotton competition for land, the production situation of cash crops such as co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q1/6895C12Q2600/13C12Q2600/154C12Q2525/191C12Q2531/113C12Q2565/125
Inventor 叶武威陆许可陈修贵王俊娟舒娜王德龙王帅樊伟莉郭晓宁郭丽雪
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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