Method for screening cotton drought-resistant related genes
A cotton and gene technology, applied in the field of screening cotton drought resistance-related genes
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[0050] Example 1. Using Methylation Sensitive Amplified Polymorphism (MSAP) to Screen Upland Cotton Drought Resistance Related Genes
[0051] 1. Test materials
[0052] The present invention adopts H177 among the cotton drought-resistant materials, adopts the sand culture method, and grows seedlings in an incubator (light for 14 hours, 30° C.; dark for 10 hours, 25° C.). Select seeds with plump and uniform seeds, disinfect them with 5% sodium hypochlorite, rinse them with sterile water, germinate them in a sterile petri dish for 36 hours, and select seeds with consistent germination for planting. Drought stress was carried out when the cotton seedlings grew to three leaves and one heart, and the water content of sandy soil was monitored. When the relative soil water content dropped to about 7.0%, leaf samples were taken, quick-frozen in liquid nitrogen, and stored at -80°C. Among them, the morphological changes of H177 before and after drought stress in cotton are as follows:...
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