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Disease-resistant immunoenhancement cyclo-(phenylalanine-serine) for channa argus

A technology of phenylalanine and serine, applied in cyclic peptide components, allergic diseases, climate change adaptation, etc., can solve problems such as reducing production performance, and achieve the effects of improving utilization rate, nutritional balance, and improving survival rate

Inactive Publication Date: 2015-11-25
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in high-density intensive aquaculture, facing various environmental stresses, disinfectants and drugs entering high-density aquaculture water can affect the integrity of gastrointestinal microbial flora, reduce production performance, and inhibit immune responses

Method used

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  • Disease-resistant immunoenhancement cyclo-(phenylalanine-serine) for channa argus
  • Disease-resistant immunoenhancement cyclo-(phenylalanine-serine) for channa argus
  • Disease-resistant immunoenhancement cyclo-(phenylalanine-serine) for channa argus

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Experimental program
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Effect test

Embodiment 1

[0027] The extraction of embodiment 1 ring-(phenylalanine-serine)

[0028] (1) Fermentation and fermentation product extraction:

[0029] marine bacteria Pseudomonas putida After 2 to 3 days of activation, pick an appropriate amount of single colony from the plate and inoculate it into a test tube containing 10 mL of MB liquid medium, and culture at 30°C and 150 r / min for 1.5 days with shaking. Then the bacterial solution was inoculated into a Erlenmeyer flask containing 100 mL of culture medium at an inoculum amount of 5% (V / V), and cultured with shaking at 30°C and 150 r / min for 1.5 days. The bacterial solution was inoculated into a conical flask with 1000 mL medium at an inoculum amount of 5% (V / V), and cultured with shaking at 30°C and 150 r / min for 5 days. After the expanded culture, the bacterial fermentation liquid was extracted with an equal volume of ethyl acetate containing 5% acetone, the bacterial liquid and the extract were mixed in a certain ratio, and each bo...

Embodiment 2

[0038] Embodiment 2 Materials and methods

[0039] Healthy snakeheads, about 80g, were randomly distributed in the 200L flowing water tank after being transported to the laboratory. Acclimate for 14 days before the formal test to adapt to the laboratory conditions (dissolved oxygen: 5.50±0.68mg / L; pH7.2±0.5; nitrite: 0.022±0.01mg / L; ammonia nitrogen: 0.14±0.05mg / L; 23±2℃ ), the test fish were randomly divided into control group, group I, group II and group III, with 3 repetitions in each group. The control group was fed with basic diet, and the experimental group was fed with 0.5, 1.0 and 2.0% cyclo-(phenylalanine-serine). Feed twice a day (9:00 and 15:00) at a rate of 3% of body weight.

[0040] Feed formula of the control group by weight: imported fish meal 15.23, domestic fish meal 15.6, cotton meal 7.5, rapeseed meal 6.3, silkworm chrysalis 4.4, cuttlefish meal 2.4, hemoglobulin meal 3.2, soybean meal 18.62, vitamin premix 1.0, peanut meal 9.68, Flour 25.42, fish oil 2....

Embodiment 3

[0048] Example 3 Blood samples for immunoassay

[0049] Blood samples were collected at 7, 14 and 21 d. Therefore, a total of 15 fish were taken from each tank in each treatment group for immunoassay. After the test fish were euthanized by injecting an overdose of MS-222, blood was collected from the tail vein with a 2.5mL syringe. Each fish was sampled only once to avoid the influence of multiple blood sampling and hand squeezing on the detection. Leukocyte separation refers to the method of Ardóet et al. (2009).

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Abstract

The invention discloses a disease-resistant immunoenhancement cyclo-(phenylalanine-serine) for channa argus, provides a composite feed for channa argus, in which pseudomonas putida metabolin cyclo-(phenylalanine-serine) is added so as to improve the disease-resistant ability of the channa argus and promote growth. After the channa argus is fed with the feed, through immunological detection, the serum lysozyme activity, serum phagocytosis ability and serum bactericidal ability of channa argus in an experimental group are obviously improved respectively up to 78.26U / mL, 63.52U / mL and 75.69U / mL, which are higher than those of a control group (respectively 17.23U / mL, 20.96U / mL and 39.36U / mL). Relative expression of IL-1beta gene of channa argus is up-regulated to 25.81, while relative expression of IL-1beta gene of channa argus in the control group is only 2.45; and by injecting medial lethal concentration of bacteria into fish belly to detect the disease resistance, the accumulative death rate of the experimental group after 15 days is 36% minimally, while that of the control group is 68%, and the survival rate of the experimental group is higher than that of the control group obviously. A comprehensive breeding method provided by the invention, matched with cultivation, makes full use of a water body, and an immunoenhancement is added, thus improving immunity and promoting growth, and enhancing economic benefits.

Description

technical field [0001] The invention belongs to the field of aquaculture, and relates to a disease-resistant immune enhancer for snakeheads and a comprehensive breeding method thereof. Background technique [0002] Snakehead ( Channaargus ) belongs to Perciformes, Channaidae, Channa genus. Yinwu snakehead is a variety that is suitable for the unique climate and water characteristics of Northeast China and has strong stress resistance. Because of its high nutritional value, delicious taste, and various eating methods, it is a very popular variety in domestic and foreign markets. In recent years, breakthroughs have been made in the domestication and artificial breeding of snakeheads, and the nutritional composition of compound feeds has been systematically studied. However, in high-density intensive aquaculture, facing various environmental stresses, disinfectants and drugs entering high-density aquaculture water can affect the integrity of gastrointestinal microbial flora, ...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/10A23K1/14A23K1/16A01K61/00A61K38/12A61P37/04
CPCY02A40/81Y02A40/818Y02P60/87
Inventor 王桂芹郑伟闫先春夏长革冯伟志刘凡宁田佳鑫杨秋实
Owner JILIN AGRICULTURAL UNIV
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