Method for establishing tree shrew periodontitis model

Abstract

The invention discloses a method for establishing a tree shrew periodontitis model. The method comprises the following steps of (1) selecting an adult healthy male tree shrew for modeling, the weight of the male tree shrew being 130-200g; a random control method being adopted, 40 tree shrews being divided into a normal control group and an experimental group, observation being carried out for 1-8 weeks, and 3-8 tree shrews being arranged in each group at each time point; the control group being not subjected to any treatment, and the other tree shrews being subjected to silk thread ligation on first molars of the lower jaws on the right sides of the tree shrews by using 2-0 silk threads; (2) 1-8 weeks after ligation, observing and recording the clinical manifestation of the tree shrew at the ligation position at the time point of each week, killing the tree shrew, and collecting the lower jawbone of the tree shrew; and (3) detecting the absorption degree of bone tissues by Micro-CT scanning, detecting the infiltration degree of periodontal tissue inflammatory cells by HE staining, detecting the infiltration degree of osteoclasts by TRAP staining, and detecting the expression conditions of inflammation-related proteins TNF-alpha, IL-1alpha and MPO and osteoclast-related protein Cathepsin K by IHC staining.

Description

technical field [0001] The invention relates to the technical field of oral biology, in particular to a method for establishing a tree shrew periodontitis model. Background technique [0002] Periodontitis is one of the most common inflammatory diseases in humans. The periodontal pathogenic bacteria in the dental plaque infect the susceptible host, triggering the host's immune response, causing the periodontal support tissue (gingiva, periodontal ligament, alveolar bone and cementum) destruction, manifested as periodontal tissue inflammation, periodontal pocket formation, loss of attachment and alveolar bone resorption, is the main cause of tooth loss in adults, and increases the risk of cardiovascular disease, neurodegenerative disease, Autoimmune diseases, adverse pregnancy outcomes, and the risk of systemic diseases such as cancer seriously affect human health and quality of life. According to the data of the fourth national oral health epidemiological survey in my count...

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Problems solved by technology

[0005] The tree shrew is an animal of the tree shrew family tree shrew. The Latin scientific name of the tree shrew: Tupaia belangeri, the English scientific name: TreeShrew. Its whole-genome sequencing analysis and a number of research results at home and abroad show that its genetic relationship is the closest to that of primates (about 93.4 %), similar to humans in terms of tissue anatomy, physiology, biochemistry, nervous system (brain function), metabolic system and immune system, and compared with primates, it has its own small size, short reproductive cycle, The characteristics of easy experimental operation and low feeding cost have become the research hotspots of scholars at home and abroad in recent years. They have been widely used in the establishment of animal models and mechanism studies of human diseases such as hepatitis and depression. However, tree shrews have not yet been used as model animals. A report on the study of oral periodontitis

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