Lung cancer cell radiation adaptability MicroRNA expression profile detection method

Abstract

The invention provides a lung cancer cell radiation adaptability MicroRNA expression profile detection method, and belongs to the technical field of cancer cell radiation adaptability detection. The method comprises the following steps of specimen selection, dividing target lung cancer cells into an experimental group and a control group, sequentially sequentially irradiating the experimental group by adopting low-dose and high-dose rays, irradiating the control group by adopting high-dose rays until the cells are digested, and carrying out low-temperature preservation; extracting total RNA, adding an RNA extraction reagent into target lung cancer cell lines in the experimental group and the control group, cracking the target lung cancer cells, centrifuging, sucking supernate, and oscillating to obtain a water-soluble precipitate; and processing and analyzing the difference of expression profiles of the lung cancer cell MicroRNA after different doses of irradiation by using an miRNA chip. The method can be used for pre-judging the effectiveness of x-ray radiotherapy on cells before treatment and judging the adaptability change of individuals to x-ray radiation in the treatment process.

Description

technical field [0001] The invention relates to the technical field of cancer cell radiation adaptability detection, in particular to a method for detecting the radiation adaptability of lung cancer cells MicroRNA expression profile. Background technique [0002] The morbidity and mortality of lung cancer in my country rank first among urban malignant tumors, and its morbidity and mortality are increasing year by year, which has attracted more and more attention from researchers. Conventional treatments for lung cancer include surgery, chemotherapy, radiotherapy, biological therapy, molecular targeted therapy, and traditional Chinese medicine. According to clinical statistics, when non-small cell lung cancer is diagnosed, only 20% of cases can undergo radical surgery, and the 5-year survival rate of surgically resected cases is only 30% to 40%. Postoperative radiotherapy can improve the local control rate and survival rate of lung cancer patients to a certain extent, so it ...

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Problems solved by technology

At the same time, the detection of radiation adaptability of lung cancer cells has important theoretical significance and practical value for improving and guiding clinical tumor radiation therapy and enhancing the therapeutic effect. At present, there is no detection method of microRNA expression profile for radiation adaptability of lung cancer cells at home and abroad.

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