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A method for constructing a model based on liver metabolomics to study the enhanced immune mechanism of selenized aminopolysaccharides in black sea bream

A metabolomics, amino selenide technology, applied in the field of biomedical analysis, can solve the problems of complex operation, low accuracy, poor systematicness, etc., and achieve the effects of simple operation, high accuracy and low cost

Active Publication Date: 2022-04-08
舟山出入境检验检疫局综合技术服务中心
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Problems solved by technology

[0005] The present invention aims at overcoming the technical problems such as poor system, low accuracy, high cost and complicated operation in the current evaluation method for studying the immune mechanism of selenized amino polysaccharides on black sea bream, and proposes a method based on liver metabolomics to study selenium Construction method of a model for enhancing the immune mechanism of black sea bream with aminopolysaccharides

Method used

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  • A method for constructing a model based on liver metabolomics to study the enhanced immune mechanism of selenized aminopolysaccharides in black sea bream
  • A method for constructing a model based on liver metabolomics to study the enhanced immune mechanism of selenized aminopolysaccharides in black sea bream
  • A method for constructing a model based on liver metabolomics to study the enhanced immune mechanism of selenized aminopolysaccharides in black sea bream

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Embodiment 1

[0040] A method for constructing a model for enhancing the immune mechanism of black sea bream based on the study of selenized amino polysaccharides based on liver metabolomics, the method comprising the following steps:

[0041] 1) Stop feeding the juvenile black sea bream for 1 day, and then select juvenile black sea bream with healthy body, uniform size, and initial body weight of 12.8-13.2 g and randomly divide them into the experimental group and the blank group. The blank group is fed with ordinary feed, In the experimental group, 0.6 mg Se / kg selenized aminopolysaccharide was added to the common feed for 8 weeks. After the end, the juvenile black sea bream was starved for 24 hours, anesthetized with 60 mg / L MS-222, and the liver tissue was taken out. Among them, 10 parallel samples were selected for each of the experimental group and the blank group, and were quickly frozen with liquid nitrogen after weighing, and then stored in an ultra-low temperature freezer at -80°C ...

Embodiment 2

[0050] The difference with Example 1 is that in step 1), the experimental group added 0.5mg Se / kg selenized amino polysaccharide in the common feed, and fed it for 10 weeks; step 2) was to get the above-mentioned 20 spare liver tissues in a centrifuge tube , add 15 times the volume of methanol frozen at -10°C, homogenize at 10,000rpm for 0.5min, centrifuge at 12,000rpm at 4°C for 5min, take the supernatant and dry it with nitrogen at 30°C, and use methanol aqueous solution (methanol and water The volume ratio of the above-mentioned liver tissue samples is 4:1) to prepare the liver tissue samples, and the preparation of the liver tissue samples is completed within 12 minutes; take the supernatants in the preparation of the above-mentioned liver tissue samples and mix them evenly, dry them with nitrogen at 50°C, and wash them with methanol The aqueous solution was redissolved to prepare quality control samples.

Embodiment 3

[0052] The difference with Example 1 is that in step 1), the experimental group added 0.7mg Se / kg selenized amino polysaccharide in the common feed, and fed it for 9 weeks; step 2) was to get the above-mentioned 20 spare liver tissues in a centrifuge tube , add 5 times the volume of methanol frozen at -30°C, homogenize at 14000rpm for 0.5min, then centrifuge at 14000rpm for 3min at 4°C, take the supernatant and dry it with nitrogen at 50°C, and use methanol aqueous solution (methanol and water The volume ratio of the liver tissue sample is 4:1) to prepare the liver tissue sample, and the preparation of the liver tissue sample is completed within 12 minutes; the supernatant in the preparation of the above-mentioned liver tissue samples is mixed evenly, dried with nitrogen at 30°C, and washed with methanol The aqueous solution was redissolved to prepare quality control samples.

[0053]Before preprocessing the analytical data, the quality and validity of the obtained analytical ...

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Abstract

A method for constructing a model for enhancing the immune mechanism of black sea bream by studying selenized aminopolysaccharide based on liver metabolomics, relates to the field of biomedical analysis, and includes the following steps: 1) dividing black sea bream juveniles into an experimental group and a blank group equally; 2 ) Selenized amino polysaccharide was added to the feed of the experimental group; 3) The juvenile black sea bream in the experimental group and the blank group were starved, and after anesthesia, the liver tissue was taken out and stored for future use; 4) The liver tissue samples were prepared and the quality Control samples were collected and processed using ultra-high performance liquid chromatography-time-of-flight mass spectrometry; 5) The collected metabolomic data of the liver of black sea bream were analyzed and processed to identify and screen out the information on the enhanced immune mechanism of selenized aminopolysaccharides on black sea bream. liver metabolomic biomarkers, and construct and analyze the metabolic pathways pointed to by the liver metabolomic biomarkers. The present invention has good systematicness, high accuracy, low cost and simple operation.

Description

technical field [0001] The invention relates to the field of biomedical analysis, in particular to a method for constructing a model for enhancing the immune mechanism of selenized amino polysaccharides to black sea bream based on liver metabolomics. Background technique [0002] As an essential trace element in the body, selenium plays an important role in the growth of the body and the maintenance of the health of the body in the form of selenoproteins and cofactors of selenium-dependent enzymes. At present, organic selenium, which has relatively low biological toxicity and is easy to be absorbed and utilized, is studied more, such as selenized amino polysaccharides. Selenized amino polysaccharides are obtained by combining polysaccharides with inorganic selenium through chemical modification methods. The physiological activity and pharmacological function of selenium and polysaccharides are optimized. The biological activity is generally higher than that of polysaccharide...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/06G01N30/14G01N30/34G01N30/86G01N30/88
CPCG01N30/02G01N30/72G01N30/06G01N30/14G01N30/34G01N30/8686G01N30/88G01N2030/146G01N2030/062G01N2030/8813G01N2030/8818G01N2030/8836G01N2030/8831G01N2570/00G01N2560/00
Inventor 周秀锦张静邵宏宏晁铎源宋立玲
Owner 舟山出入境检验检疫局综合技术服务中心
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