Method for detecting fibrinolytic function of blood

Abstract

The invention relates to a method for detecting the fibrinolysis function of blood. The method comprises the following steps: a, preparing an experimental group reagent and a control group reagent; b,performing absorbance detection; and c, calculating the fibrinolysis capacity value and the fibrinolysis time value. The fibrinolysis function of blood can be directly detected; the method is relatively simple to operate; the fibrinolysis function of the blood can be quantitatively evaluated, namely the fibrinolysis capacity and the fibrinolysis time are reflected; and traditional fibrinolytic markers such as FDP and D dimers are high in non-specificity and affected by inflammation, bleeding, thrombus load and the like, and the real fibrinolytic ability can be reflected, so that the specificity is high. In addition, the method is not influenced by blood chyle, jaundice, hemolysis and other factors, and the anti-interference capability is high.

Description

technical field [0001] The invention relates to the technical field of blood fibrinolysis function detection, in particular to a blood fibrinolysis function detection method. Background technique [0002] Fibrin clumps form after blood coagulation, which needs to be dissolved by the fibrinolytic system to allow blood vessels to recanalize. Weakened fibrinolytic function can lead to thrombosis, such as venous thrombosis caused by congenital fibrinolytic defects, and venous thrombosis caused by reduced fibrinolysis after orthopedic surgery. Hyperfibrinolysis can cause bleeding, such as post-traumatic hemorrhage, hemorrhage after urinary system surgery, and severe obstetric hemorrhage are all related to hyperfibrinolysis. Tumor patients may show fibrinolysis inhibition or hyperfibrinolysis, and clinically, hemorrhage or thrombosis may occur. [0003] The blood system maintains balance by the coagulation system, anticoagulation system, and fibrinolysis system. Patients neithe...

AI Technical Summary

Problems solved by technology

They are all indirect detection of fibrinolytic activation molecules to represent the degree of fibrinolytic activation, which is highly non-specific and affected by inflammation, bleeding, thrombus load, etc., not fibrinolytic function detection

[0004] The detection of existing fibrinolytic markers cannot represent the real-time level of fibrinolytic function

Because fibrinolytic marker concentration depends on substrate concentration, enzyme concentration, liver and kidney function and metabolic effects, it cannot reflect real-time plasmin activity

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