Antirabies virus IgG (Immunoglobulin G) antibody ELISA (Enzyme Linked Immunosorbent Assay) detection kit for dogs

Abstract

The invention relates to an antirabies virus IgG (Immunoglobulin G) antibody kit for dogs. In the kit, an enzyme label plate is coated with anti-rabies virus monoclonal antibodies in advance, wherein a coating buffer solution is 0.05M of carbonate buffer solution with pH of 9.6, and the coating amount is 0.1-1ug per hole; 1-10% of BSA (Bull Serum Albumin) or skimmed milk in percentage by mass concentration is used as a blocking buffer; rabies virus purified antigens are coated after blocking, wherein the coating amount is 0.1-1ug per hole; 0.01mol / L PBS (Phosphate-Buffered Saline) with pH 7.2-7.4 containing 0.1-10% of BSA and 0.01-0.05% of NaN3 in percentage by mass concentration is used as a sample diluent; a horse radish peroxidase-rabbit anti-rabies IgG enzyme conjugate is used as an enzyme conjugate; 0.01mol / L PBS with pH 7.2-7.4 containing tween-20 with volume concentration of 0.05% is used as a washing concentrate; an enzyme substrate A solution is a 3,3'-5,5'-tetramethyl benzidine solution, and an enzyme substrate B solution is a hydrogen peroxide solution; a 1 mol / L H2SO4 solution is adopted as a stop solution; and a positive control and a negative control are arranged in the kit. With 100% of specificity and 1:640 of sensitivity, the kit is used for the immune effect evaluation of experimental dogs, pet dogs and common dogs which are inoculated with rabies vaccines.

Description

technical field [0001] The invention relates to a detection kit, in particular to an ELISA detection kit for canine anti-rabies virus IgG antibody. Background technique [0002] Rabies is a zoonotic infectious disease caused by Rabies virus (RV), and its main hosts are dogs, cats and other animals. The typical symptom is hydrophobia, also known as: hydrophobia. The disease is extremely dangerous, and the case fatality rate is almost 100%. Dogs are not only used for hunting, detection, guarding, carnivorous and as pets, dogs are also one of the important experimental animals widely used in the fields of life science, medicine, pharmacy, chemical industry, agriculture, military, environmental protection, aerospace and bioengineering. With the development of the economy and the improvement of people's living standards, the number of pet dogs is not only increasing, but also the area of ​​activity of dogs is constantly expanding. According to the information obtained from the...

AI Technical Summary

Problems solved by technology

The recombinant protein has the disadvantages of difficult renaturation, lack of spatial conformational epitopes, and the disadvantage of difficult purification of the whole virus, which will lead to low detection sensitivity

Fluorescence immunoassay, radioimmunoassay and chemiluminescence method require certain experimental instruments, and radioimmunoassay still has the problem of radioisotope contamination

Although colloidal gold immunochromatography has the advantage of simple operation, its sensitivity is relatively low.

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