Human anti-rabies virus IgG antibody ELISA test kit

Abstract

The invention relates to a human anti-rabies virus IgG antibody ELISA test kit. An ELISA plate is firstly coated with an anti-rabies virus monoclonal antibody, wherein the coating buffer solution is a 0.05M carbonate buffer solution of which the pH value is 9.6, and the coating amount is 0.1-1ug per hole; a blocking solution is a BSA or skimmed milk of which the mass concentration is 1-10%; the ELISA plate is coated with a rabies virus purified antigen after being blocked, wherein the coating amount is 0.1-1ug per hole; a sample diluent is a 0.01mol / L phosphate buffer solution (PBS) which contains bovine serum albumin (BSA) with a mass concentration of 0.1-10% and NaN3 with a mass concentration of 0.01-0.05 and has a pH value of 7.2-7.4; an enzyme conjugate is a horse radish peroxidase-mouse anti-human IgG enzyme conjugate; a concentrated cleaning solution is a 0.01mol / L PBS which contains tween-20 with a volume concentration of 0.05% and has a pH value of 7.2-7.4; a zymolyte A solution is a 3,3'-5,5'-tetramethyl benzidine solution, and a zymolyte B solution is an oxydol solution; and a stop solution is a 1mol / L H2SO4 solution, and a positive control and a negative control are arranged in the kit. The specificity of the kit is up to 100%, and the sensitivity is 1:640. The kit is used for evaluating the immunity effect of humans inoculated with rabies vaccines.

Description

technical field [0001] The invention relates to a detection kit, in particular to a human anti-rabies virus IgG antibody ELISA detection kit. Background technique [0002] Rabies is a zoonotic infectious disease caused by Rabies virus (RV), and its main hosts are dogs, cats and other animals. The typical symptom is hydrophobia, also known as: hydrophobia. The disease is extremely dangerous, and the case fatality rate is almost 100%. With the development of the economy and the improvement of people's living standards, the number of pet dogs is not only increasing, but also the area of ​​activity of dogs is constantly expanding. According to the information obtained from the pet medical market, people are eager to know whether pet dogs can gain protection after being vaccinated against rabies. However, the current detection of rabies virus antibody has not been widely used due to the limitation of detection conditions and costs. Rabies virus is the pathogen of rabies, and m...

AI Technical Summary

Problems solved by technology

The recombinant protein has the disadvantages of difficult renaturation, lack of spatial conformational epitopes, and the disadvantage of difficult purification of the whole virus, which will lead to low detection sensitivity

Fluorescence immunoassay, radioimmunoassay and chemiluminescence method require certain experimental instruments, and radioimmunoassay still has the problem of radioisotope contamination

Although colloidal gold immunochromatography has the advantage of simple operation, its sensitivity is relatively low.

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