42 results about "Rabies Virus Antigen" patented technology

This invention provides one dog rabies virus antigen glue gold immune chromatography test paper and its process, which comprises the following steps: according to antigen antibody immune combination basic principle to label the sheep IgG by glue gold label covering on the glass fiber film as combination pad; covering the purification rabies virus antigen and gene engineer expressed antigen and rabit IgG covering on the NC film as test line and quality control line; when testing the antibody, forming antibody combination to expose red band.

The invention relates to a kind of hydrophobia vaccine for human, which in detail relates to a freeze-drying hydrophobia vaccine for human and its preparation method. It is contained in physiological soluble buffer solution with pH being 7-8, the sodium chloride weight proportion is 0.4-1.0%, one or several from mycose, sucrose, gelatin, maltose and dextran is used as stabilizing agent, the concentration is 0.5-5%; the weight proportion of shaping agent for freeze-drying agent is 1-5%, and the purified hydrophobia viral antigen concentration is 4-20 IU per ml.

The invention provides a method for making rabies virus antigen. The method comprises the following steps that: the antigen gene of rabies virus or the combined expression combination of the antigen gene is respectively cloned in a baculovirus carrier so as to obtain a transfer expression carrier; the transfer expression carrier and baculovirus undergo cotransfection so as to carry out homologous recombination or transposition, thereby obtaining recombined baculovirus; the recombined baculovirus is used to infect insect host and cell; the infected insect host is cultured to express corresponding rabies antigen; and the expressed antigen is ingathered and purified so as to obtain rabies virus antigen. The method adopts a baculovirus expression system to make safe and efficient rabies virus antigen in a domestic silkworm bioreactor; moreover, due to having extremely high safety, the made antigen can be directly used to make injection vaccine and oral vaccine used for animal immunization. The method can substantially reduce the production cost of rabies virus antigen, and has the advantages of safety, high efficiency, less energy consumption and low cost, etc.

The invention provides a method for preparing a vaccine composition. The method comprises the following steps: (1) preparing a liquid containing a porcine pseudorabies virus; (2) adding a nonionic surfactant or a solution containing the nonionic surfactant into the liquid in the step (1) and solubilizing envelope proteins in the liquid containing the porcine pseudorabies virus; (3) removing the nonionic surfactant in the step (2) to obtain a purified porcine pseudorabies virus antigen; and (4) preparing the vaccine composition by using the antigen prepared in the step (3). The invention further provides the vaccine composition containing porcine pseudorabies antigen prepared by the preparation method. Immunosuppression components are removed from the vaccine composition and meanwhile, immunity active ingredients are retained to the maximum extent. The vaccine composition has a relatively good protective effect on porcine lung diseases; when the vaccine composition and a live virus antigen of porcine reproductive and respiratory syndrome are jointly acted, the vaccine composition has no inhibiting effect to the live virus antigen, showing that the vaccine composition can be jointly used with various other antigen components.

The invention discloses a kit for detecting a rabies virus antibody, which belongs to the technical field of biological diagnosis. The kit comprises a magnetic bead coupled with a rabies virus antigen, a second antibody combined with the rabies virus antibody and provided with a marker, a rabies virus antibody standard product, a rabies virus antibody quality control product, a sample dilute solution, and a washing solution, wherein the DNA sequence of the rabies virus antigen is as shown by Seq No. 1, and an amino acid sequence of the rabies virus antigen is as shown by Seq No. 2. By adoptingthe kit, a to-be-detected substance can be separated by adopting a tubular magnetic ball, then the detection is performed by adopting a chemical luminescent method, the interference of impurities canbe avoided, the possibility of false positive can be reduced, and the detection sensitivity can be improved.

The invention provides a method for detecting canine rabies virus antibody (IgG) and a detection kit. The kit is composed of a coated plate and a reagent reaction system, and comprises a rabies virus antigen-coated reaction plate, a standard substance, positive contrast serum, a washing lotion (20X), a sample weak solution, an enzyme-marked combination substance, a developer A, a developer B and a stopping solution. The method is characterized in that the method uses an ELISA method to determine that the content of the canine rabies virus antibody (IgG) reaches a absorbance corresponding to a protective level by detecting the standard substance of the kit, and by compared the absorbance of the sample to be detected with the absorbance of the standard substance, the content of the canine rabies virus antibody (IgG) contained in the sample to be detected is judged whether to reach an immunization protective level. The method and the kit can simultaneously detect a lot of samples, and a detection result is remarkably with a result by a neutralization experiment, has high accuracy degree, is suitable for monitoring an immunization inoculation effect and determining an individual immunization state, and can be used for investigating animal eqpidemic diseases.

The invention relates to a porcine circovirus II type-porcine pseudorabies double-combination vaccine which is characterized by including at least one porcine circovirus II type antigen and at least one porcine pseudorabies virus antigen. The invention also relates to two preparation methods of the double-combination vaccine. One method comprises the steps: inactivating the porcine circovirus II type to obtain a porcine circovirus II type inactivated vaccine; and mixing the porcine pseudorabies virus antigen with a freeze-drying protective agent to obtain a porcine pseudorabies freeze-drying living vaccine, and then dissolving the porcine pseudorabies freeze-drying living vaccine with the inactivated vaccine as a diluent to obtain the porcine circovirus II type-porcine pseudorabies double-combination vaccine composition. The other method comprises the steps: mixing the porcine circovirus II type antigen with the porcine pseudorabies virus antigen in proportion, and then mixing with the freeze-drying protective agent to obtain the double-combination freeze-drying vaccine. With use of the double-combination vaccine, the cold chain operating cost can be greatly reduced; and the combination vaccine has a synergistic role in the immune effect on the porcine pseudorabies virus antigen, and does not affect the immune effect of the porcine circovirus antigen.

The invention discloses a piece of test paper for colloidal gold immunochromatograohic assay of an IgG (immunoglobulin G) antibody of dog anti-rabies virus and a preparation method of the test paper. The test paper comprises a sample absorbing region, a gold-labeled probe region, a solid-phase antibody region, a water absorbing region and a supporting plate, wherein the sample absorbing region, the gold-labeled probe region, the solid-phase antibody region and the water absorbing region are laid on the supporting plate and are partially overlapped in sequence; the sample absorbing region is coated with rabies virus antigen; the gold-labeled probe region is coated with a gold-labeled probe 1 and a gold-labeled probe 2 which are a monoclonal antibody of the colloidal gold-labeled rabies virus and rabbit IgG respectively; the solid-phase antibody region has a control line C1 (coated with a goat anti-rabbit IgG antibody), a testing line T (coated with a goat anti-dog IgG antibody), a control line C2 (coated with a goat anti-rabbit IgG antibody) and a control line C3 (coated with a goat anti-mouse IgG antibody). The test paper disclosed by the invention has the advantages that the test is fast, the accuracy rate is high, the specificity is strong, the test paper is simple and convenient to carry and operate, and the level of the rabies virus antibody is judged according to the color depths of the control lines.

The invention discloses a porcine parvovirus-porcine pseudorabies combined inactivated vaccine, and a preparation method and an application thereof. The preparation method comprises the following steps: respectively inactivating a porcine parvovirus liquid and a porcine pseudorabies liquid with pyrrole to obtain a porcine parvovirus antigen and a porcine pseudorabies antigen, and mixing the porcine parvovirus antigen and the porcine pseudorabies antigen with an oil phase adjuvant to prepare the combined inactivated vaccine. No immunosuppression exists between the two antigen components in the combined inactivated vaccine, the combined inactivated vaccine prepared from the porcine parvovirus antigen and the porcine pseudorabies antigen according to a volume ratio of 2:3 has better safety and immunoprotection effects than single vaccines, and the porcine parvovirus and the porcine pseudorabies can be substantially immunoprotected through one-shot syringe injection. The porcine parvovirus-porcine pseudorabies combined inactivated vaccine has the advantages of good safety, avoiding of untoward effects of multi-time immunoprophylaxis, reduction of the immunization cost, and use convenience, and can be applied in effective prevention of the porcine parvovirus and the porcine pseudorabies.

The invention belongs to the technical field of veterinary biological products and particularly relates to a pseudorabies/porcine parvovirus infection combined inactivate vaccine and a suspension culture preparation method. The preparation method comprises the following steps: preparing a pseudorabies virus suspension culture antigen and a porcine parvovirus infection virus suspension culture antigen; proportionally mixing the inactivated pseudorabies virus and porcine parvovirus infection virus antigen solutions, and then adding an adjuvant to fully emulsify to obtain the pseudorabies/porcineparvovirus infection combined inactivate vaccine. The suspension culture processes of the pseudorabies virus antigen solution and the porcine parvovirus infection virus antigen solution are established, the virus antigen solutions prepared through the suspension culture process have the advantages of high antigen content, large batch of the antigen and stable batch, the preparation method greatlyreduces the use of manpower, the occupied area and space of a culture system is reduced, and the production cost of an enterprise is lowered; meanwhile, the pseudorabies/porcine parvovirus infectioncombined inactivate vaccine is used, two viruses are prevented through one syringe, the immunity times of an animal are reduced, the stress times of the animal are reduced, and the production cost ofthe vaccine and the culture cost of a farmer are greatly reduced.

The invention provides a method for preparing a rabies virus antigen by chloroplast, comprising the following steps: (1) constructing a chlamydomonas chloroplast expression vector of rabies virus antigen gene; (2) screening the constructed expression vector transgenosis chlamydomonas to obtain transgenosis chlamydomonas; and (3) culturing transgenosis chlamydomonas to express the rabies virus antigen gene in chlamydomonas to obtain the rabies virus antigen. In the method of the invention, a chlamydomonas chloroplast expression system is utilized to produce rabies virus antigens safely and efficiently in a chlamydomonas bioreactor, the expressed protein has high specificity and good immunity effect, and can not produce non-purposive immunoreaction. The cost of the method of the invention is significantly lower than that of a traditional method for preparing the rabies virus antigen, the method has no need of investing and building factories and no generation of three wastes (waste gas, waste water and industrial residue), and the consumption of electric power, water resource and other resources is extremely low. The antigen prepared by the method of the invention has very high security and no need of purification, and can be directly used as animal supplementary feed, thus having treatment effect.

The invention discloses a rabies virus antibody quantitative detection kit as well as a preparation method and a detection method thereof, belongs to the technical field of antibody detection kits, and solves the problems that the antibody detection accuracy is not high enough and potential safety hazards exist in the prior art. The kit comprises an immunochromatographic detection test strip and asample diluent, the immunochromatographic detection test strip sequentially comprises a sample pad coated with a rabies virus antigen, a marker pad coated with a monoclonal antibody of the rabies virus antigen, a nitrocellulose membrane coated with a detection line and a quality control line and a water absorption pad from one end to the other end, and the sample pad, the marker pad, the nitrocellulose membrane and the water absorption pad are mutually stacked and attached to the upper surface of a back plate. According to the invention, the rabies virus antibody in blood is detected by adopting a competition method, and real antibody numerical values in a blood sample can be directly displayed according to a standard curve in combination with strip reading equipment, so that the detection accuracy and sensitivity are improved.

The invention discloses a rabies virus antibody colloidal gold detection test strip for human and livestock joint detection and a preparation method of the rabies virus antibody colloidal gold detection test strip. The rabies virus antibody colloidal gold detection test strip comprises a colloidal gold labeled binding area and a detection area, wherein a detection line and a quality control line are arranged on the detection area, rabies virus antigens labeled by colloidal gold and albumen protein gold particles labeled by colloidal gold are wrapped by the colloidal gold labeled binding area, staphylococcus albumins are wrapped by the detection line, and ovalbumin antibodies are wrapped by the quality control line. According to the rabies virus antibody colloidal gold detection test strip, the detection cost can be reduced, the detection sensitivity can be improved, false positive can be avoided, and the human and livestock joint detection of rabies virus antibodies is achieved. Furthermore, the instability problem appearing when monoclonal antibodies or polyclonal antibodies are used as the quality control line can be effectively solved through the quality control line, and the rabies virus antibody colloidal gold detection test strip has a great significance in large-batch sample detection, field application and immunized human self-detection and has very large market development potentials.

The invention discloses a preparation method for a living-vector vaccine against rabies, a product thereof and application thereof. The method comprises the following steps: 1, designing primers and amplifying a rabies virus antigen protein gene G and a bacterial flagellin gene FIjB or FIiC; 2, inserting the antigen protein gene G and the gene FIjB or FIiC between a specific promoter and a specific terminator of a replication-competent defective adenovirus shuttle vector so as to construct an adenovirus shuttle vector containing a gene G-FIjB or a gene G-FIiC; 3, subjecting the obtained recombinant replication-competent defective adenovirus shuttle vector and an adenovirus skeleton vector to homologous recombination so as to obtain recombinant adenovirus plasmids; and 4, producing recombinant adenoviruses with a uniform genomic structure from the recombinant adenovirus plasmids. Moreover, the invention also provides the living-vector vaccine against rabies prepared by using the method. Compared with the prior art, the living-vector vaccine against rabies prepared in the invention is safer and more effective, is short in a preparation period and has good application prospects in the field of prevention or treatment of rabies.

The invention discloses rabies virus antibody test paper and a preparation method thereof and a detection method thereof, which belong to the technical field of antibody test paper. The problems of false positive risk and safety hazard in the prior art are solved. The test paper comprises, from one end to the other end, a sample pad which coats a rabies virus antigen, a gold standard pad which coats a monoclonal antibody against the rabies virus antigen, a nitrocellulose membrane which coats a detection line and a quality control line, and an absorbent pad, wherein the sample pad, the gold standard pad, the nitrocellulose membrane and the absorbent pad overlap each other and are attached to the upper surface of a backboard. The rabies virus antigen coated by the sample pad is rabies virus-like particles. According to the invention, the used labeled antigen is the rabies virus-like particles expressed by insect cells, which avoids false positives; the monoclonal antibody against the rabies virus G protein competes with a neutralizing antibody in the blood to improve the detection accuracy and sensitivity; the virus-like particles are free of nucleic acid components; and biosafety risks produced by the use of whole viruses as marker antigens are eliminated.