Method for preparing artificial antigen of 3-chlorine-1,2-propylene glycol

An artificial antigen, propylene glycol technology, applied in animal/human protein, measuring devices, instruments, etc., can solve the problems of complex operation, only laboratory operation, long detection time, etc.

Inactive Publication Date: 2006-02-01
SOUTH CHINA AGRI UNIV
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Problems solved by technology

[0003] The detection of 3-chloro-1,2-propanediol has always been mainly performed by chromatography-mass spectrometry. At present, the most advanced detection method in the world is stable isotope dilution mass spectrometry. Although this method is accurate and sensitive and has a low detection limit, its pretreatment cumbersome, complicated operation, long detection time, expensive equipment, high detection cost, can only be operated in the laboratory, and has certain limitations. Therefore, people are eager to have a simple, fast, sensitive and cheap detection technology that can detect 3-chloro -1,2-propanediol for rapid detection of a large number of samples
At present, the research on the immunoassay of chemical pollutants at home and abroad has involved pesticides, veterinary drugs, biological toxins and other objects, but there is no report on the immunoassay for 3-chloro-1,2-propanediol

Method used

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Embodiment

[0016] 3-Chloro-1,2-propanediol artificial antigen preparation:

[0017] (1) In order to achieve the above object, the present invention takes the following measures: configure ethylenediamine solution: configure 10% (v / v) ethylenediamine solution 1mL, adjust pH to 6.0 with 6mol / L hydrochloric acid.

[0018] (2) Ethylenediamine activated carrier: add 3mL 6.1mg / mL bovine serum albumin or polypeptide or synthetic polypeptide or semi-synthetic polypeptide aqueous solution to the ethylenediamine solution, then add 0.8mg water-soluble carbodiimide hydrochloride, React at room temperature for 15 minutes.

[0019] (3) Desalting: After equilibrating the Sephadex G-25 column with 0.15mol / L NaCl solution, put the carrier protein solution activated by ethylenediamine on the column, monitor the absorption at 280nm by ultraviolet light, and collect the first peak component.

[0020] (4) Concentration: Concentrate the collected solution to 2 mL with a dialysis bag, and transfer the concent...

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Abstract

A method for preparing 3 ¿C chlorine ¿C 1, 2 ¿C propanediol artificial antigen includes using 3 ¿C chloro ¿C lactic acid as hapten, using water soluble carbon diimine hydrochloride as coupling agent and coupling hapten of 3 ¿C chloro ¿C lactic acid with cationization carrier to prepare out 3 ¿C chlorine , 2 ¿C propanediol artificial antigen.

Description

technical field [0001] The invention relates to the preparation of an artificial antigen, especially the preparation of the artificial antigen of 3-chloro-1,2-propanediol. Background technique [0002] 3-Chloro-1,2-propanediol, abbreviated as 3-MCPD, is usually called trichloropropanol. It is a product formed by replacing one hydroxyl group of glycerol with a chlorine atom. It is a chloropropane that has attracted much attention in recent years. Alcohol is a kind of organic pollutant. 3-Chloro-1,2-propanediol is mainly derived from acid hydrolyzed vegetable protein (HVP). HVP hydrolyzate is often added to soy sauce as a flavoring agent. In addition, some fast food, solid soup, baked grain, rye and black 3-Chloro-1,2 -Propylene glycol, chloropropanols sometimes appear during home cooking and processing and storage of some foods. Many studies have shown that 3-chloro-1,2-propanediol can cause cancer, affect kidneys and fertility, cause anemia, leukopen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/536G01N33/544C07K14/76
Inventor 黄晓钰雷红涛孙远明吴青王弘谌国莲高秀杰陈家贵肖治理
Owner SOUTH CHINA AGRI UNIV
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