Process for preparing hyaluronic acid-chitosan crosslinked biocompatible materials

A technology of chitosan cross-linking and hyaluronic acid, applied in the field of biochemistry, can solve the problems of non-biocompatibility, single function, and no multiple biological functions, and achieve the effect of expanding application fields and high biological stability

Inactive Publication Date: 2006-10-11
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the derivatives of hyaluronic acid cross-linked by this method have a single function and do not have multiple biological functions, especially such biological materials are not completely biocompatible

Method used

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  • Process for preparing hyaluronic acid-chitosan crosslinked biocompatible materials
  • Process for preparing hyaluronic acid-chitosan crosslinked biocompatible materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 0.2g (0.5mM) hyaluronic acid and 0.2g (1.25mM) chitosan were respectively dissolved in 20ml 0.1M HCl solution and stirred overnight. Add solid NaCl with a final concentration of 2M to the dissolved hyaluronic acid hydrochloride solution, stir well to dissolve. Weigh 0.0956g (0.5mM) EDC and 0.0575g (0.5mM) NHS, dissolve them in 5ml ultrapure water, then pour into the hyaluronic acid hydrochloric acid solution, and stir for 30min. Then inject the chitosan hydrochloric acid solution into the hyaluronic acid hydrochloric acid solution, and constantly and fully stir. When the solution is stable and uniform, slowly add liquid N,N,N',N'-tetramethylethylenediamine to the mixed solution to adjust the pH to 5.0. Stir at room temperature for 12h. The stirred product was loaded into a dialysis bag with a molecular weight cut-off of 14,000, dialyzed in 1M NaCl solution for 2 days, and then dialyzed in ultrapure water for one day. The dialysis product was freeze-dried to obtain a ...

Embodiment 2

[0020] 0.1 g of hyaluronic acid and 0.2 g of chitosan were dissolved in 20 ml of 0.1 M HCl solution and stirred overnight. Add solid NaCl with a final concentration of 2M to the dissolved hyaluronic acid hydrochloride solution, stir well to dissolve. Weigh 0.0478g EDC and 0.0288g NHS, dissolve them in 5ml ultrapure water, then pour into the hyaluronic acid hydrochloric acid solution, and stir for 30min. Then inject the chitosan hydrochloric acid solution into the hyaluronic acid hydrochloric acid solution, and constantly and fully stir. When the solution is stable and uniform, slowly add liquid N,N,N',N'-tetramethylethylenediamine to the mixed solution to adjust the pH to 4.5. Stir at room temperature for 9h. The stirred product was loaded into a dialysis bag with a molecular weight cut-off of 14,000, dialyzed in 1M NaCl solution for 2 days, and then dialyzed in ultrapure water for one day. The dialysis product was freeze-dried to obtain a mesh material.

Embodiment 3

[0022] 0.2 g of hyaluronic acid and 0.1 g of chitosan were dissolved in 20 ml of 0.1 M HCl solution, and stirred overnight. Add solid NaCl with a final concentration of 2M to the dissolved hyaluronic acid hydrochloride solution, stir well to dissolve. Weigh 0.0956g EDC and 0.0575g NHS, dissolve them in 5ml ultrapure water, then pour into the hyaluronic acid hydrochloric acid solution, and stir for 30min. Add liquid N,N,N',N'-tetramethylethylenediamine to the two polysaccharide solutions respectively to adjust the pH to 4.0. Then the chitosan hydrochloric acid solution was injected into the hyaluronic acid hydrochloric acid solution and stirred at room temperature for 6 h. The stirred product was loaded into a dialysis bag with a molecular weight cut-off of 14000, dialyzed in 1M NaCl solution for 2 days, and then dialyzed in ultrapure water for 1 day. The dialysis product was freeze-dried to obtain a mesh material.

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Abstract

A method for preparation of hyaluronic acid -chitose cross compatible material belongs to the biochemical technical sphere. The invention selects water-soluble carbodiimide as cross linker and N-hydroxy succinimide as active agent, adjusts PH value with N,N,N',N'-tetramethylethylenediamine, then makes the hyaluronic acid and the chitose perform cross-linking reaction, finally prepares the hyaluronic acid -chitose cross compatible material by dialysis and cryodesiccation. The material has a good application prospect in domains of drug embedment, tissue pack, film material and so on.

Description

technical field [0001] The invention discloses a preparation method of a hyaluronic acid-chitosan cross-linked biocompatible material, which belongs to the technical field of biochemistry. Background technique [0002] Both hyaluronic acid and chitosan are polysaccharides with excellent biocompatibility, and they have special biochemical effects in living organisms. For example, hyaluronic acid can be used as a joint lubricant and a wound healing agent; chitosan also has an antibacterial effect as a wound healing agent. The above two polysaccharides can also be applied in the fields of drug carriers and tissue fillers. [0003] Since all organisms contain hyaluronidase, ordinary hyaluronic acid is extremely easy to decompose in the organism. For this reason, scholars at home and abroad have proposed chemical modification methods to improve the anti-degradation performance of hyaluronic acid. Most of them choose the method of cross-linking small molecular chemical substanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L5/08A61K47/36C08J3/24C08K5/20
Inventor 陈坚邓禹刘龙堵国成李秀芬刘登如
Owner JIANGNAN UNIV
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