15 results about "Target enrichment" patented technology

This invention relates to the field of bioinformatics processing and discloses a method and system for mining the structure-activity relationship (SMR) of porcine neurotrophic peptides based on sequence features. The method includes constructing an original sample index table and fusing multi-source production data, extracting peptide sequence features to generate a sequence feature matrix, constructing a sequence-process joint graph containing peptide nodes and process state nodes, training a structure-activity relationship graph neural network to mine SMR relationships, and deriving a process control decision table based on a process response sample set generated by the network, thereby achieving online optimization of the porcine neurotrophic peptide preparation process. This invention solves the problem of SMR mining caused by the separation of process parameters, sequence information, and activity data, achieving accurate characterization of the synergistic effect of sequence and process and reverse optimization of process parameters, thus improving the targeted enrichment efficiency and bioactivity retention level of target neurotrophic peptides.

This invention discloses a mitochondrial-targeted nanomaterial for senescent cells and its application in anti-aging. The material is a nucleic acid aptamer-tetrahedral DNA, mainly assembled from four single-stranded DNA sequences, SEQ ID Nos. 1-4. Preparation involves first hybridizing the four single-stranded DNA sequences, then concentrating them to obtain aptamer-modified tetrahedra, which are then coupled with tannic acid (TA) via a Mann reaction in the presence of formaldehyde to obtain the Apt-tFNAs-TA complex. Finally, it is incubated with excess catechins to obtain the final delivery material, Apt-tFNAs@catechin-TA. This invention's material exhibits good biocompatibility and skin tissue compatibility, significantly improving uptake efficiency and mitochondrial localization ability in senescent cells. It can effectively deliver polyphenolic compounds such as catechins and protocatechuic acid, improving mitochondrial function and addressing the technical problems of low delivery efficiency of active ingredients and insufficient targeted enrichment in senescent cells.

This invention discloses a targeted sequencing method for multiple pathogenic microorganisms, belonging to the field of biotechnology. The targeted sequencing method for multiple pathogenic microorganisms includes the following steps: sample pretreatment; targeted enrichment; PCR amplification; high-throughput sequencing; and bioinformatics analysis. This invention solves the problems of existing technologies, such as inability to accurately type pathogens, detect drug resistance genes, and analyze virulence genes, as well as poor targeting and slow analysis speed. This invention provides high-purity target nucleic acids for PCR amplification through a triple targeted enrichment system; the anti-inhibition multiplex PCR system ensures efficient amplification of target nucleic acids in complex samples; high-throughput sequencing enables rapid sequencing of target nucleic acids; and the bioinformatics analysis module enables simultaneous completion of pathogen typing, drug resistance gene detection, and virulence gene analysis, significantly improving overall detection efficiency and meeting the clinical needs for rapid, accurate, and comprehensive pathogen detection.

This invention discloses a method for preparing DNA samples for SNP chip detection, comprising the following steps: obtaining at least one library sequence, wherein the library sequence includes a capture sequence complementary to the target fragment of the DNA sample to be tested; amplifying the library sequence and then digesting it with enzymes to obtain a single-stranded library sequence; mixing the amplification product of the DNA sample to be tested with the single-stranded library sequence, denaturing and annealing; and digesting the product with Exonuclease I. The DNA sample prepared by this invention is more purified, with targeted enrichment of the target fragment and removal of a large number of non-target fragments. When applied to SNP chip detection, it improves the hybridization efficiency with the SNP chip, thereby increasing the detection signal value and accuracy.

This invention provides a method for targeted enrichment of target sequences in a spatial transcriptome cDNA library. The method includes: using a hybridization capture probe set to hybridize and extend a target nucleic acid sequence or its complementary sequence on the target sequence in the spatial transcriptome cDNA library to obtain an extension product; wherein the hybridization capture probe set includes two or more probes, and any two probes in the hybridization capture probe set do not overlap in the hybridization region of the target sequence; the extension direction of any probe in the hybridization capture probe set is from the target nucleic acid sequence or its complementary sequence to the spatial sequence or its complementary sequence; the extension product includes: the spatial sequence or its complementary sequence, and part or all of the target nucleic acid sequence or its complementary sequence; the extension product is isolated to obtain an enriched library. This method solves the problem in existing technologies that only unbiased sequencing can be performed on spatial transcriptome libraries, and targeted sequencing cannot be achieved, making it applicable to the field of spatial transcriptome library enrichment.

The application provides a product, a method and an application for mutant DNA enrichment / detection, and belongs to the technical field of biological medicines.The application provides a primer group for enriching mutant DNA based on a PCR reaction, and realizes enrichment of mutant DNA based on the primer group.The mutant DNA enrichment method provided by the application realizes efficient enrichment of mutations through a hairpin competition reaction, and lays a foundation for high-sensitivity detection of mutations.The application further combines a CRISPR / Cas14a detection technology, provides a double-hairpin competition CRISPR / Cas14a system, realizes supersensitive detection of mutations, can realize co-amplification of multiple mutant targets, has the advantages of supersensitive detection and multi-target enrichment, and can be used for detection of small solid tumors or micro residual lesions.

This invention discloses a method for in vitro expansion and culture of TCR cells targeting viral antigens, belonging to the field of in vitro immune cell culture technology. The specific steps of this method are as follows: first, peripheral blood mononuclear cells are isolated, and the initial activation of targeted T cells is completed and a culture system is established to start expansion; samples are taken at preset nodes, and the abundance of targeted and non-targeted TCR clones is calculated by single-cell TCR sequencing; culture parameters are dynamically adjusted according to abundance thresholds, and targeted enrichment is performed when non-targeted clones exceed the limit; the operation is repeated until the end of the expansion cycle and the cells are harvested. This invention integrates targeted clone abundance detection into the culture process by using fixed-node micro-sampling sequencing throughout the expansion process, combined with three-level graded threshold dynamic control of culture parameters, and dual-detection abundance correction. When non-targeted clones exceed the limit, they are enriched in real time, locking in the proliferative advantage of targeted clones, reducing cell viability loss during sorting, minimizing batch quality differences, and stably producing high-purity targeted TCR cells that meet clinical requirements.

This invention provides a method for detecting integration sites and quantitatively assessing viral integration efficiency based on targeted capture sequencing. The method includes targeted enrichment and sequencing of samples using a targeted capture sequencing panel; performing virus-host dual-reference alignment and breakpoint clustering on the enriched sequencing data to achieve comprehensive detection of integration sites; and calculating the integration efficiency by comparing the abundance of virus-host fusion reads at each integration site with the total amount of virus-specific reads. This allows for simultaneous quantitative assessment of integration site profiles and integration efficiency in the same sequencing experiment. Compared to the LAM-PCR method, the TES Panel of this invention significantly improves the sensitivity and comprehensiveness of fragmented integration detection through multi-site, multi-fragment targeted capture, providing key technical support for quality control of gene therapy products, risk warning of viral infection-related diseases, and safety assessment.

The present invention belongs to the technical fields of DNA methylation library construction and DNA methylation detection, and specifically relates to a method for constructing a high-throughput sequencing library for enriched methylated DNA and use thereof. The construction method, on the basis of existing high-throughput sequencing library construction methods, incorporates two steps of restriction endonuclease digestion, and, combined with a specially designed linker, can achieve selective library construction for methylated CpG. Compared with existing conventional high-throughput sequencing library construction methods, the construction method incorporates only a small number of steps, making the overall procedure relatively simple. Moreover, high-throughput sequencing is directly performed on the prepared library, so that methylation states in widely distributed intervals on the whole genome can be obtained, thereby eliminating cumbersome steps of base conversion and targeted enrichment.

This invention discloses a liquid-phase probe-targeted capture library construction method and kit for enteroviruses, belonging to the field of virus detection technology. This invention combines targeted enrichment with high-throughput sequencing, featuring broad coverage, high flexibility, high sensitivity, and high specificity, enabling multi-dimensional information output including quantitative, qualitative, and in-depth analysis. Through the probe-targeted capture "enrichment-screening" mechanism, this invention reduces probe redundancy design, minimizes background nucleic acid interference from intestinal flora, exhibits excellent adaptability to complex samples, and eliminates the need for complex sample pretreatment, making it suitable for large-scale epidemiological detection.

This invention relates to the field of biotechnology, and particularly to primers and methods for whole-genome sequencing of African swine fever virus (ASFV) based on next-generation sequencing platforms and targeted enrichment. This invention discloses a primer set and method for whole-genome sequencing of ASFV based on next-generation sequencing platforms and targeted enrichment, comprising 32 pairs of specific targeted amplification primers. These primers exhibit high specificity and sensitivity, fully leveraging the advantages of next-generation sequencing—high throughput, low cost, high accuracy, and mature technology—to accurately and completely obtain the entire ASFV genome sequence. This invention can obtain complete genetic information of ASFV, identify and diagnose infections of different ASFV genotypes, and, by analyzing the genetic differences among different strains, determine the geographical origin and transmission dynamics of circulating strains, tracing the evolution of recombinant strains. It provides resources for basic research on virus-host interactions and drug resistance, and offers a theoretical basis for developing long-term prevention and control strategies. It also promotes innovation in diagnostic methods.

The application relates to the field of biotechnology, in particular to a method for constructing a spatial transcriptome sequencing library based on an immune repertoire, which realizes targeted enrichment and high-throughput sequencing of TCR / BCR sequences through library molecule circularization and two rounds of enrichment, and is used for observing the space-time atlas of gene expression and immune response of immune cells.

The invention discloses a low-abundance cell nucleus enrichment method which comprises the following steps: (1) splitting a tissue sample to obtain a cell nucleus with extracellular cytoplasm retained; and (2) marking and sorting the cell nucleuses obtained in the step (1) to obtain enriched low-abundance cell nucleuses. According to the method, a mild dissociation technology is used, so that more cytoplasm components can be reserved in cell nucleuses, and then the cell nucleuses are used for marking cytoplasm proteins and are combined with flow sorting, so that directional enrichment of the cell nucleuses with low abundance and specific functional states can be effectively realized. Through the whole process optimization, the method can ensure the completeness of the cell nucleus to the maximum extent, takes downstream sequencing application into consideration, and can obtain high-quality mononuclear transcriptome sequencing data by combining with the screening of cytoplasm sequencing data. Moreover, according to the method, directional enrichment is realized through the cytoplasmic protein, the use cost can be effectively reduced, and the method is suitable for large-scale clinical sample data acquisition and has higher flux compared with a single space transcriptome slice.