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Construction method of genome presumptive area nucleic acid sequencing library and device thereof

A predetermined region and nucleic acid sequencing technology, which is applied to a system for determining nucleic acid sequences in a predetermined region of a genome, construction of a nucleic acid sequencing library in a predetermined region of the genome, and the construction of a nucleic acid sequencing library in a predetermined region of the genome, which can solve the problem that the SNP detection and typing method needs to be improved.

Active Publication Date: 2016-06-01
BGI SHENZHEN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current SNP detection and typing methods still need to be improved

Method used

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  • Construction method of genome presumptive area nucleic acid sequencing library and device thereof
  • Construction method of genome presumptive area nucleic acid sequencing library and device thereof
  • Construction method of genome presumptive area nucleic acid sequencing library and device thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0138] According to the general method described above, the figure 1 The shingled probe (4X) design method shown in b prepares probes as a control, and performs target SNP typing on goats, as follows:

[0139] 1. Probe design and synthesis

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PUM

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Abstract

The invention discloses a construction method of a genome presumptive area nucleic acid sequencing library and a device thereof. The method comprises the following steps: genomic DNA undergoes fragmentation treatment; DNA fragment undergoes fragment selection; the DNA fragment which has undergone fragment selection successively undergoes dephosphorylation, first end repairing and connection with first sequencing linker; a first connection product is screened by a probe; target fragment successively undergoes double-strands cyclization treatment and enzyme digestion; and an enzyme digestion product successively undergoes second end repairing, connection with second sequencing linker and DNA double-strands separation treatment so as to obtain single-stranded DNA which forms a genome presumptive area sequencing library, wherein the probe satisfies at least one condition selected from ten conditions. The probe has good specificity, high sensitivity and coverage in the aspect of target enrichment. By the method, the sequencing library for SNP enrichment detection can be effectively constructed.

Description

technical field [0001] The present invention relates to the field of biotechnology, especially the field of sequencing technology, in particular to a method and device for constructing a nucleic acid sequencing library in a predetermined region of the genome, and more specifically, to a method for constructing a nucleic acid sequencing library in a predetermined region of the genome, and a method for determining the nucleic acid sequence in a predetermined region of the genome The method, the device for constructing the nucleic acid sequencing library of the predetermined region of the genome, and the system for determining the nucleic acid sequence of the predetermined region of the genome. Background technique [0002] SNP (Single Nucleotide Polymorphism), that is, single nucleotide polymorphism marker, also known as single nucleotide polymorphism, refers to the difference of a single base in a DNA sequence. The meaning of a SNP is that in a given population, more than 1% ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12M1/34C12N15/11C12N15/10C40B50/06C40B60/14
Inventor 陈晓丽何诗阳方东明王晓雯王勇斯原辉
Owner BGI SHENZHEN CO LTD
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