Construction method of genome presumptive area nucleic acid sequencing library and device thereof

Abstract

The invention discloses a construction method of a genome presumptive area nucleic acid sequencing library and a device thereof. The method comprises the following steps: genomic DNA undergoes fragmentation treatment; DNA fragment undergoes fragment selection; the DNA fragment which has undergone fragment selection successively undergoes dephosphorylation, first end repairing and connection with first sequencing linker; a first connection product is screened by a probe; target fragment successively undergoes double-strands cyclization treatment and enzyme digestion; and an enzyme digestion product successively undergoes second end repairing, connection with second sequencing linker and DNA double-strands separation treatment so as to obtain single-stranded DNA which forms a genome presumptive area sequencing library, wherein the probe satisfies at least one condition selected from ten conditions. The probe has good specificity, high sensitivity and coverage in the aspect of target enrichment. By the method, the sequencing library for SNP enrichment detection can be effectively constructed.

Description

technical field [0001] The present invention relates to the field of biotechnology, especially the field of sequencing technology, in particular to a method and device for constructing a nucleic acid sequencing library in a predetermined region of the genome, and more specifically, to a method for constructing a nucleic acid sequencing library in a predetermined region of the genome, and a method for determining the nucleic acid sequence in a predetermined region of the genome The method, the device for constructing the nucleic acid sequencing library of the predetermined region of the genome, and the system for determining the nucleic acid sequence of the predetermined region of the genome. Background technique [0002] SNP (Single Nucleotide Polymorphism), that is, single nucleotide polymorphism marker, also known as single nucleotide polymorphism, refers to the difference of a single base in a DNA sequence. The meaning of a SNP is that in a given population, more than 1% ...

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Problems solved by technology

[0003] However, the current SNP detection and typing methods still need to be improved

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