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Polygene multi-target enrichment method

A multi-target, multi-gene technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem of no TSH inhibition scheme recommendation

Inactive Publication Date: 2015-08-19
TIANJIN TUMOR HOSPITAL +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In addition, the application of TSH inhibitors after thyroid cancer surgery has been observed to reduce the bone mineral density of female patients one year after surgery. In addition, for those patients with bone and cardiovascular diseases before the diagnosis of thyroid cancer, currently There are no TSH suppression regimens recommended for these patients

Method used

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Embodiment Construction

[0116] The present invention will be further described below in conjunction with the accompanying drawings. It should be noted that this embodiment is based on the technical solution, and provides detailed implementation and specific operation process, but the protection scope of the present invention is not limited to the present invention. Example.

[0117] Such as figure 1 As shown, the implementation process of the present invention is specifically as follows:

[0118] Pre-Library preparation (50ng DNA sample)

[0119] 1. gDNA fragmentation and adapter adapter

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Abstract

The invention discloses a polygene multi-target enrichment method. A capture probe library is designed, and target gene sequences are enriched in a probe capture way at a DNA (deoxyribonucleic acid) level, so that detection on all variation types, such as mutation, deletion, insertion, fusion and copy number amplification, of target genes is realized, and the defect that presently the target gene sequences are enriched by a PCR method at the DNA level, only mutation and deletion of the target genes can be detected, and the fusion cannot be detected is overcome.

Description

technical field [0001] The invention relates to the technical field of biological detection, and specifically designs a multi-gene multi-target enrichment method for targeted preparation of thyroid samples for high-throughput sequencing. Background technique [0002] Thyroid cancer is the most common malignancy among endocrine system and head and neck tumors. New cases of thyroid cancer account for 1%-5% of all cancers every year. The incidence of thyroid cancer in women is higher than that in men, which is about three times that of men. The age of onset of thyroid cancer is relatively young, and the incidence rate increases with age. In the past 30 years, the incidence of thyroid cancer has continued to increase rapidly, which has aroused widespread concern. [0003] Thyroid cancer is divided into papillary carcinoma (PTC), follicular carcinoma (FTC), Hnrthle cell carcinoma, medullary carcinoma (MTC) and undifferentiated carcinoma (ATC) according to its pathological charac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6806C12Q1/6869C12Q2523/308C12Q2531/113C12Q2535/122
Inventor 高明于津浦于洋汉雨生揣少坤段飞蝶
Owner TIANJIN TUMOR HOSPITAL
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