Protein or polypeptide drug-loaded tissue adhesive film and preparation method thereof

A sticking film and drug-loading technology, which is applied in the field of biomedicine, can solve the problems of failure to achieve clinical treatment effects, difficulty in effective control of sustained release, lack of drug delivery technology, etc., to achieve good degradable absorbability, direct drug delivery, high Biosecurity Effects

Inactive Publication Date: 2016-08-31
苏州博创同康医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] With the rapid development of science and technology, a large number of new types of drugs are constantly being discovered, but the biggest obstacle preventing the clinical use of many new drugs is the lack of effective drug delivery technology
Many drug delivery technologies in the prior art have problems such as being unable to truly target the lesion, and slow release is difficult to effectively control. These problems will lead to systemic drug delivery, large dosage, large side effects, poor targeting, and small drug dose entering the cell. Cannot achieve clinical treatment effect

Method used

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  • Protein or polypeptide drug-loaded tissue adhesive film and preparation method thereof
  • Protein or polypeptide drug-loaded tissue adhesive film and preparation method thereof
  • Protein or polypeptide drug-loaded tissue adhesive film and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] In a sterile workbench, prepare a 12 cm diameter petri dish with a built-in silicon wafer membrane of the same diameter (washed, sterilized and depyrogenated), and dry; respectively prepare solutions A (1 mg / kg) of materials with cationic groups. ml chitosan, 0.1mol / L acetic acid, 0.2mol / L NaCl, pH=4) and liquid B (1mg / ml carboxymethyl chitosan, 0.15mol / L NaCl, pH=6) with anionic group material Take part B liquid and add small interfering nucleic acid drug (eGFP-siRNA (sense: 5'-GGCACAAGCUGGAGUACAAUU-3'; antisense: 5'-UUGUACUCCAGCUUGUGCCUU-3', 20ug / mL) and cell targeting factor (hyaluronic acid, molecular weight Less than 20,000 Daltons, 10ug / mL) and targeting peptide (1×10 -4 mg / ml) was formulated into liquid C, and the amino acid sequence of the targeted polypeptide was: valine-glycine-valine-alanine-proline-glycine; the above solutions were respectively filled into high-pressure instantaneous spraying equipment, first Spray liquid C into the petri dish with high pre...

Embodiment 2

[0054] In a sterile workbench, prepare a plate of polymer material (washed, sterilized and depyrogenated), and dry; respectively prepare solutions A of materials with cationic groups (2 mg / ml chitosan, 0.1 mol / L acetic acid , 0.2mol / L NaCl, pH=4) and B solution (alginic acid of 2mg / ml, 60,000 to 80,000 molecular weight, 0.15mol / L NaCl, pH=6) with anionic group material is referred to as B solution; B solution Then add small interfering nucleic acid drug (same as Example 1, 20ug / mL) and cell targeting factor (hyaluronic acid, molecular weight is less than 20,000 Daltons, 10ug / mL), and then add targeting polypeptide (1 ×10 -4 mg / ml), the amino acid sequence of the targeted polypeptide is: isoleucine-lysine-valine-alanine-valine; first immerse the plate in solution A for 20 minutes, take it out into the cleaning solution, wash and dry; Then immerse the plate in solution B for 30 minutes, take it out, wash and dry it with cleaning solution, do this alternately 160 times, and fina...

Embodiment 3

[0056] In a sterile workbench, prepare a plate of polymer material (washed, sterilized and depyrogenated), and dry; respectively prepare solutions A of materials with cationic groups (2 mg / ml chitosan, 0.1 mol / L acetic acid , 0.2mol / L NaCl, pH=4) and B solution with anionic group material (2mg / ml hyaluronic acid, 0.15mol / L NaCl, pH=6) referred to as B solution; Nucleic acid cationic liposome (cationic lipid preparation method refers to "gene transfection mediated by anionic liposome-cationic liposome complex", Journal of Pharmaceutical Practice, 2011 (29): 4, small interfering nucleic acid is the same as Example 1 , small interfering nucleic acid cationic liposome addition amount is 0.5mg / ml, small nucleic acid drug load 21.7%) stir evenly and targeting polypeptide (1 * 10 -4 mg / ml), the amino acid sequence of the targeted polypeptide is: arginine-glycine-aspartic acid; first immerse the plate in solution A for 20 minutes, take it out into the cleaning solution, wash and dry; ...

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Abstract

The invention relates to the field of biomedicine, especially to a drug-loaded tissue adhesive film and a preparation method thereof. The invention provides a drug-loaded tissue adhesive film, which comprises alternately superimposed cationic layers and anionic layers, at least one layer of the cationic layers and anionic layers is a drug layer, or at least one layer of the cationic layers and anionic layers contains a charged drug. The drug-loaded tissue adhesive film provided by the invention has good tissue adhesiveness, good biocompatibility and degradable absorbability, and good stability, and the physical and chemical properties of the drug-loaded tissue adhesive film can be adjusted by regulating the material composition.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to a protein or polypeptide drug-loaded tissue adhesive film and a preparation method thereof. The drug-loaded tissue adhesive film can be surgically implanted into the patient's tissue for local direct sustained release and controlled release. medicines to treat various diseases. Background technique [0002] With the rapid development of science and technology, a large number of new types of drugs are constantly being discovered, but the biggest obstacle preventing the clinical use of many new drugs is the lack of effective drug delivery technology. Many drug delivery technologies in the prior art have problems such as being unable to truly target the lesion, and slow release is difficult to effectively control. These problems will lead to systemic drug delivery, large dosage, large side effects, poor targeting, and small drug dose entering the cell. Can not achieve clinical tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K47/36A61K47/42A61K31/7105A61K31/44A61K31/404A61K31/7032A61K38/17A61K38/20A61K31/517A61K31/506A61K31/4439A61K31/513A61K39/395A61K38/16A61K38/18A61K31/727A61K31/715
CPCA61K9/7007A61K9/0024A61K31/404A61K31/44A61K31/4439A61K31/506A61K31/513A61K31/517A61K31/7032A61K31/7105A61K31/715A61K31/727A61K38/16A61K38/17A61K38/1808A61K38/1825A61K38/185A61K38/1866A61K38/2013A61K39/395A61K47/36A61K47/42
Inventor 刘光万吴昌琳
Owner 苏州博创同康医药科技有限公司
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